{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,9,17]],"date-time":"2025-09-17T16:54:38Z","timestamp":1758128078582,"version":"3.41.2"},"reference-count":72,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2020,12,28]],"date-time":"2020-12-28T00:00:00Z","timestamp":1609113600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81872125"],"award-info":[{"award-number":["81872125"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["American J Rep Immunol"],"published-print":{"date-parts":[[2021,3]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Problem<\/jats:title><jats:p>The mechanism underlying endometriosis is currently unknown. However, studies have indicated that immunity plays an important role in endometriosis occurrence and development. Long non\u2010coding RNAs (lncRNAs) do not encode proteins but participate in a variety of biological processes via different mechanisms. This study investigated differences in immune cells and immune\u2010related lncRNAs via high\u2010throughput RNA sequencing (RNA\u2010seq) analysis of ectopic and eutopic endometria with endometriosis.<\/jats:p><\/jats:sec><jats:sec><jats:title>Method of study<\/jats:title><jats:p>RNA\u2010seq was performed in six pairs of ectopic and eutopic endometria samples, and real\u2010time quantitative polymerase chain reaction was used to verify the results of RNA\u2010seq for 30 pairs of samples. Different immune cell types were identified based on the RNA\u2010seq results, using ImmuCellAI. Immune\u2010related lncRNAs were obtained by analyzing immune\u2010related genes from the ImmPort Database and RNA\u2010seq results.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>A total of 952 differentially expressed lncRNAs were identified, of which 446 were immune\u2010related. The ectopic and eutopic endometrium could easily be distinguished in the principal component analysis of immune\u2010related lncRNAs. Analysis of 24 immune cell types revealed the differential abundance of 13 types. Sixty immune\u2010related mRNAs were associated with the top 20 dysregulated immune\u2010related lncRNAs, 11 of which were transcripts of immune cell marker genes.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>Our data indicated that a variety of dysregulated lncRNAs were associated with immunity, and these may provide a basis for future immune\u2010related endometriosis research.<\/jats:p><\/jats:sec>","DOI":"10.1111\/aji.13359","type":"journal-article","created":{"date-parts":[[2020,10,16]],"date-time":"2020-10-16T14:24:51Z","timestamp":1602858291000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["RNA sequencing\u2010based long non\u2010coding RNA analysis and immunoassay in ovarian endometriosis"],"prefix":"10.1111","volume":"85","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-4913-0386","authenticated-orcid":false,"given":"Jianlei","family":"Bi","sequence":"first","affiliation":[{"name":"Department of Obstetrics and Gynecology Shengjing Hospital of China Medical University Shenyang China"},{"name":"Department of Obstetrics and Gynecology The Second Hospital of Dalian Medical University Dalian China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dandan","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology Shengjing Hospital of China Medical University Shenyang China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Liangyi","family":"Cui","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology Shengjing Hospital of China Medical University Shenyang China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Qing","family":"Yang","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology Shengjing Hospital of China Medical University Shenyang China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2020,12,28]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(04)17403-5"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0015-0282(16)57733-4"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.fertnstert.2004.10.058"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1210\/jc.81.8.3112"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1097\/01.gco.0000136496.95075.79"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1097\/AOG.0b013e3181758ec6"},{"issue":"2","key":"e_1_2_8_8_1","first-page":"93","article-title":"Metastatic or embolic endometriosis, due to the menstrual dissemination of endometrial tissue into the venous circulation","volume":"3","author":"Sampson JA","year":"1927","journal-title":"Am J Pathol"},{"issue":"2","key":"e_1_2_8_9_1","first-page":"151","article-title":"Retrograde menstruation in healthy women and in patients with endometriosis","volume":"64","author":"Halme J","year":"1984","journal-title":"Obstet Gynecol"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ejogrb.2016.04.021"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1155\/2015\/795976"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature01266"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tcb.2011.04.001"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.18632\/aging.102874"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13578-019-0346-3"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.1093\/molehr\/gaz040"},{"issue":"4","key":"e_1_2_8_17_1","first-page":"2269","article-title":"LINC00261 functions as a competing endogenous RNA to regulate BCL2L11 expression by sponging miR\u2010132\u20103p in endometriosis","volume":"11","author":"Wang H","year":"2019","journal-title":"Am J Transl Res"},{"key":"e_1_2_8_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.yexcr.2019.05.010"},{"key":"e_1_2_8_19_1","doi-asserted-by":"publisher","DOI":"10.1002\/mrd.22581"},{"key":"e_1_2_8_20_1","doi-asserted-by":"publisher","DOI":"10.1126\/scisignal.2000568"},{"key":"e_1_2_8_21_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0100690"},{"key":"e_1_2_8_22_1","doi-asserted-by":"publisher","DOI":"10.1093\/hmg\/ddh245"},{"key":"e_1_2_8_23_1","unstructured":"http:\/\/bioinfo.life.hust.edu.cn\/ImmuCellAI"},{"key":"e_1_2_8_24_1","doi-asserted-by":"publisher","DOI":"10.1002\/advs.201902880"},{"key":"e_1_2_8_25_1","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btp616"},{"key":"e_1_2_8_26_1","doi-asserted-by":"publisher","DOI":"10.2147\/JPR.S207599"},{"key":"e_1_2_8_27_1","doi-asserted-by":"publisher","DOI":"10.1155\/2018\/7924021"},{"key":"e_1_2_8_28_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.trsl.2012.05.001"},{"key":"e_1_2_8_29_1","doi-asserted-by":"publisher","DOI":"10.1111\/jog.12050"},{"key":"e_1_2_8_30_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijgo.2007.08.018"},{"key":"e_1_2_8_31_1","doi-asserted-by":"publisher","DOI":"10.1177\/1933719114525271"},{"key":"e_1_2_8_32_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00404-014-3599-4"},{"key":"e_1_2_8_33_1","doi-asserted-by":"publisher","DOI":"10.1093\/humrep\/deu123"},{"key":"e_1_2_8_34_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.fertnstert.2010.06.038"},{"key":"e_1_2_8_35_1","doi-asserted-by":"publisher","DOI":"10.1155\/2013\/240702"},{"key":"e_1_2_8_36_1","doi-asserted-by":"publisher","DOI":"10.1007\/s43032-020-00205-7"},{"issue":"1","key":"e_1_2_8_37_1","article-title":"Immunomodulation inhibits the development of endometriosis in rats","volume":"71","author":"Dera\u2010Szymanowska A","year":"2020","journal-title":"J Physiol Pharmacol"},{"key":"e_1_2_8_38_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.rbmo.2020.05.013"},{"key":"e_1_2_8_39_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2011.07.014"},{"key":"e_1_2_8_40_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms16023251"},{"issue":"1","key":"e_1_2_8_41_1","first-page":"24","article-title":"Comprehensive RNA sequencing of healthy human endometrium at two time points of the menstrual cycle","volume":"96","author":"Sigurgeirsson B","year":"2017","journal-title":"Biol Reprod"},{"key":"e_1_2_8_42_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms19072120"},{"issue":"8","key":"e_1_2_8_43_1","first-page":"4422","article-title":"LncRNA RP11\u2010395G23.3 suppresses the endometrial cancer progression via regulating microRNA\u2010205\u20105p\/PTEN axis","volume":"12","author":"Xin W","year":"2020","journal-title":"Am J Transl Res"},{"key":"e_1_2_8_44_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.lfs.2020.117295"},{"key":"e_1_2_8_45_1","doi-asserted-by":"publisher","DOI":"10.1038\/srep23343"},{"key":"e_1_2_8_46_1","doi-asserted-by":"publisher","DOI":"10.15252\/emmm.201505245"},{"key":"e_1_2_8_47_1","doi-asserted-by":"publisher","DOI":"10.1038\/nri2873"},{"key":"e_1_2_8_48_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI118815"},{"key":"e_1_2_8_49_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1281554"},{"key":"e_1_2_8_50_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrc909"},{"key":"e_1_2_8_51_1","doi-asserted-by":"publisher","DOI":"10.1530\/rep.0.1230217"},{"key":"e_1_2_8_52_1","doi-asserted-by":"publisher","DOI":"10.1096\/fj.07-9034com"},{"key":"e_1_2_8_53_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.rbmo.2013.12.011"},{"key":"e_1_2_8_54_1","doi-asserted-by":"publisher","DOI":"10.1177\/1933719114525267"},{"key":"e_1_2_8_55_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1471-4914(03)00051-0"},{"key":"e_1_2_8_56_1","doi-asserted-by":"publisher","DOI":"10.2174\/092986711794088416"},{"key":"e_1_2_8_57_1","doi-asserted-by":"publisher","DOI":"10.1111\/aji.12408"},{"key":"e_1_2_8_58_1","doi-asserted-by":"publisher","DOI":"10.1155\/2016\/2916070"},{"key":"e_1_2_8_59_1","doi-asserted-by":"publisher","DOI":"10.3390\/jcm8091468"},{"key":"e_1_2_8_60_1","doi-asserted-by":"publisher","DOI":"10.1038\/nm1093"},{"key":"e_1_2_8_61_1","doi-asserted-by":"publisher","DOI":"10.1084\/jem.20040165"},{"key":"e_1_2_8_62_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.175.12.8392"},{"key":"e_1_2_8_63_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2008.05.009"},{"key":"e_1_2_8_64_1","doi-asserted-by":"publisher","DOI":"10.1038\/nri2889"},{"key":"e_1_2_8_65_1","doi-asserted-by":"publisher","DOI":"10.1093\/humrep\/des346"},{"key":"e_1_2_8_66_1","doi-asserted-by":"publisher","DOI":"10.1093\/humupd\/dmz014"},{"key":"e_1_2_8_67_1","doi-asserted-by":"publisher","DOI":"10.1096\/fj.201900797R"},{"key":"e_1_2_8_68_1","doi-asserted-by":"publisher","DOI":"10.1093\/molehr\/gay045"},{"key":"e_1_2_8_69_1","doi-asserted-by":"publisher","DOI":"10.1530\/REP-18-0424"},{"key":"e_1_2_8_70_1","doi-asserted-by":"publisher","DOI":"10.1111\/jcmm.13947"},{"key":"e_1_2_8_71_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ejogrb.2019.03.020"},{"key":"e_1_2_8_72_1","doi-asserted-by":"publisher","DOI":"10.1111\/cpr.12397"},{"key":"e_1_2_8_73_1","doi-asserted-by":"publisher","DOI":"10.1111\/aji.13074"}],"container-title":["American Journal of Reproductive Immunology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/aji.13359","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/aji.13359","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/aji.13359","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,2]],"date-time":"2023-09-02T19:00:54Z","timestamp":1693681254000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/aji.13359"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,12,28]]},"references-count":72,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2021,3]]}},"alternative-id":["10.1111\/aji.13359"],"URL":"https:\/\/doi.org\/10.1111\/aji.13359","archive":["Portico"],"relation":{},"ISSN":["1046-7408","1600-0897"],"issn-type":[{"type":"print","value":"1046-7408"},{"type":"electronic","value":"1600-0897"}],"subject":[],"published":{"date-parts":[[2020,12,28]]},"assertion":[{"value":"2020-08-10","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2020-10-12","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2020-12-28","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e13359"}}