{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,8]],"date-time":"2026-04-08T23:12:23Z","timestamp":1775689943802,"version":"3.50.1"},"reference-count":35,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2020,11,24]],"date-time":"2020-11-24T00:00:00Z","timestamp":1606176000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100003725","name":"National Research Foundation of Korea","doi-asserted-by":"publisher","award":["2020R1F1A1048362"],"award-info":[{"award-number":["2020R1F1A1048362"]}],"id":[{"id":"10.13039\/501100003725","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["American J Rep Immunol"],"published-print":{"date-parts":[[2021,5]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Problem<\/jats:title><jats:p>We aimed to identify amniotic fluid (AF) proteins associated with the subsequent rupture of membranes (ROM) occurring in the absence of active labor in women with threatened preterm labor (PTL) using an antibody microarray.<\/jats:p><\/jats:sec><jats:sec><jats:title>Method of the study<\/jats:title><jats:p>This retrospective cohort study included 183 singleton pregnant women with PTL (24\u201333\u00a0weeks) who underwent amniocentesis. A nested case\u2010control study was conducted using AF samples from 20 women with subsequent ROM within 7\u00a0days of sampling (case subjects) and 20 gestational age\u2010matched women with term delivery (TD) without ROM (control subjects), via protein\u2010antibody microarray analysis. Seven candidate proteins of interest were validated via ELISA in the total cohort.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Seventeen proteins displayed significant intergroup differences. ELISA validation confirmed that the levels of EN\u2010RAGE, Fas, IL\u20108, IP\u201010, MMP\u20108, and MMP\u20109 were significantly higher, whereas IGFBP\u20103 levels were significantly lower in the AF of women with subsequent ROM within 7\u00a0days of sampling than in that of women with TD without ROM. Moreover, the time interval from sampling to membrane rupture was significantly correlated with the expression levels of AF proteins, except for IL\u201010.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Using an antibody microarray, we identified various inflammatory, angiogenic, matrix\u2010degrading, and apoptosis\u2010related proteins in the AF that were associated with subsequent ROM occurring in the absence of active labor in women with threatened PTL. These findings provide novel insights into the molecular mechanisms underlying membrane rupture without active labor in threatened PTL.<\/jats:p><\/jats:sec>","DOI":"10.1111\/aji.13371","type":"journal-article","created":{"date-parts":[[2020,11,6]],"date-time":"2020-11-06T08:48:15Z","timestamp":1604652495000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":15,"title":["Antibody microarray analysis of amniotic fluid proteins associated with subsequent ruptured membranes in women with threatened preterm labor"],"prefix":"10.1111","volume":"85","author":[{"given":"Subeen","family":"Hong","sequence":"first","affiliation":[{"name":"Department of Obstetrics and Gynecology College of Medicine Catholic University of Korea Seoul Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yehyon","family":"Park","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology Seoul National University 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