{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,22]],"date-time":"2026-07-22T11:01:11Z","timestamp":1784718071248,"version":"3.55.0"},"reference-count":48,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2021,7,20]],"date-time":"2021-07-20T00:00:00Z","timestamp":1626739200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["American J Rep Immunol"],"published-print":{"date-parts":[[2021,9]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Problem<\/jats:title>\n                    <jats:p>Inflammation and immune responses play crucial roles in the development of endometriosis. Although interleukin\u20109 (IL\u20109) has a pro\u2010inflammatory function in chronic inflammatory diseases, its function in endometriosis remains unknown. Here, we aimed to investigate the significance of IL\u20109 and IL\u20109\u2010producing lymphocytes in endometriosis.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Method of study<\/jats:title>\n                    <jats:p>\n                      Specimens were obtained from patients with and without endometriosis. Peritoneal fluid (PF), peripheral blood (PB), and ovarian endometrioma (OE) tissues were analyzed for the proportion of CD4\n                      <jats:sup>+<\/jats:sup>\n                      IL\u20109\n                      <jats:sup>+<\/jats:sup>\n                      lymphocytes and IL\u20109 concentration using flow cytometry and enzyme\u2010linked immunosorbent assay. OE, endometrium with endometriosis (EE), and normal endometrium (NE) were analyzed for IL\u20109 receptor (IL\u20109R) expression using immunohistochemical staining. IL\u20109\u2010dependent changes in Interleukin\u20108 (IL\u20108) expression in endometrial stromal cells from OE (OESCs) were evaluated using real\u2010time PCR.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      The proportion of CD4\n                      <jats:sup>+<\/jats:sup>\n                      IL\u20109\n                      <jats:sup>+<\/jats:sup>\n                      lymphocytes was higher in the PF, but not the PB, of patients with endometriosis than individuals without endometriosis (\n                      <jats:italic>p<\/jats:italic>\n                      \u00a0&lt;\u00a0.05). However, IL\u20109 levels in the PF did not differ between those with and without endometriosis. We detected CD4+ IL\u20109+ lymphocytes in OE tissues and IL\u20109R in OE tissues and OESCs. In OESC culture, IL\u20109 significantly elevated IL\u20108 expression in a dose\u2010dependent manner (\n                      <jats:italic>p<\/jats:italic>\n                      \u00a0&lt;\u00a0.05), which was nullified by the addition of the anti\u2010IL\u20109 receptor antibody. Furthermore, IL\u20109 additively stimulated IL\u20108 expression in the presence of TNF\u2010\u03b1 (\n                      <jats:italic>p<\/jats:italic>\n                      \u00a0&lt;\u00a0.05).\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>Our findings show that IL\u20109 produced by helper T cells induces IL\u20108 expression, suggesting that IL\u20109 plays an important role in the development of endometriosis by stimulating IL\u20108 expression.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/aji.13380","type":"journal-article","created":{"date-parts":[[2020,11,19]],"date-time":"2020-11-19T16:52:53Z","timestamp":1605804773000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":11,"title":["Interleukin\u20109 produced by helper T cells stimulates interleukin\u20108 expression in endometriosis"],"prefix":"10.1111","volume":"86","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-6058-594X","authenticated-orcid":false,"given":"Yosuke","family":"Tarumi","sequence":"first","affiliation":[{"name":"Department of Obstetrics and Gynecology Graduate School of Medical Science Kyoto Prefectural University of Medicine  Kyoto Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9792-4132","authenticated-orcid":false,"given":"Taisuke","family":"Mori","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology Graduate School of Medical Science Kyoto Prefectural University of Medicine  Kyoto Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hiroyuki","family":"Okimura","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology Graduate School of Medical Science Kyoto Prefectural University of Medicine  Kyoto 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