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While we have previously shown a 5\u2010fold higher cytotoxicity of RUPP NKs versus normal pregnant NKs, their role in RUPP pathophysiology remains unclear. In this study, we tested the hypotheses that (1) adoptive transfer of RUPP\u2010stimulated NKs will induce maternal hypertension and IUGR in normal pregnant control (Sham) rats and (2) adoptive transfer of Sham NKs will attenuate maternal hypertension and IUGR in RUPP rats.<\/jats:p><\/jats:sec><jats:sec><jats:title>Method of Study<\/jats:title><jats:p>On gestation day (GD)14, vehicle or 5\u00a0\u00d7\u00a010<jats:sup>6<\/jats:sup>RUPP NKs were infused i.v. into a subset of Sham rats (Sham+RUPP NK), and vehicle or 5\u00a0\u00d7\u00a010<jats:sup>6<\/jats:sup>Sham NKs were infused i.v. into a subset of RUPP rats (RUPP+Sham NK;<jats:italic>n<\/jats:italic>\u00a0=\u00a012\/group). On GD18, Uterine Artery Resistance Index (UARI) was measured. On GD19, mean arterial pressure (MAP) was measured, animals were sacrificed, and blood and tissues were collected for analysis.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Adoptive transfer of RUPP NKs into Sham rats resulted in elevated NK activation, UARI, placental oxidative stress, and preproendothelin expression as well as reduced circulating nitrate\/nitrite. This led to maternal hypertension and IUGR. RUPP recipients of Sham NKs demonstrated normalized NK activation, sFlt\u20101, circulating and placental VEGF, and UARI, which led to improved maternal blood pressure and normal fetal growth.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>These data suggest a direct role for cNKs in causing preeclampsia pathophysiology and a role for normal NKs to improve maternal outcomes and IUGR during late gestation.<\/jats:p><\/jats:sec>","DOI":"10.1111\/aji.13386","type":"journal-article","created":{"date-parts":[[2020,12,15]],"date-time":"2020-12-15T02:07:23Z","timestamp":1607998043000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":22,"title":["Adoptive transfer of placental ischemia\u2010stimulated natural killer cells causes a preeclampsia\u2010like phenotype in pregnant rats"],"prefix":"10.1111","volume":"85","author":[{"given":"Olivia 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