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Here, we characterized the immune microenvironment generated by decidualized cells in response to soluble factors secreted by blastocysts that shape the receptive milieu.<\/jats:p><\/jats:sec><jats:sec><jats:title>Method of Study<\/jats:title><jats:p>We used an <jats:italic>in vitro<\/jats:italic> model of decidualization based on the Human Endometrial Stromal Cells line (HESC) differentiated with medroxiprogesterone and dibutyryl\u2010cAMP, then treated with human blastocysts\u2010conditioned media (BCM) classified according to their quality.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Decidualized cells treated with BCM from impaired developed blastocysts increased IL\u20101\u03b2 production. Next, we evaluated the ability of decidualized cells to modulate other mediators associated with menstruation as chemokines. Decidualized cells responded to stimulation with BCM from impaired developed blastocysts increasing CXCL12 expression and CXCL8 secretion. The modulation of these markers was associated with the recruitment and activation of neutrophils, while regulatory T cells recruitment was restrained. These changes were not observed in the presence of BCM from normal developed blastocysts.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Soluble factors released by impaired developed blastocysts induce an exacerbated inflammatory response associated with neutrophils recruitment and activation, providing new clues to understand the molecular basis of the embryo\u2010endometrial dialogue.<\/jats:p><\/jats:sec>","DOI":"10.1111\/aji.13423","type":"journal-article","created":{"date-parts":[[2021,3,25]],"date-time":"2021-03-25T13:10:03Z","timestamp":1616677803000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":14,"title":["Understanding the natural selection of human embryos: Blastocyst quality modulates the inflammatory response during the peri\u2010implantation period"],"prefix":"10.1111","volume":"87","author":[{"given":"Laura","family":"Fern\u00e1ndez","sequence":"first","affiliation":[{"name":"CONICET Universidad de Buenos Aires Instituto de Qu\u00edmica Biol\u00f3gica de la Facultad de Ciencias Exactas y Naturales (IQUIBICEN) Buenos Aires Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Esteban","family":"Grasso","sequence":"additional","affiliation":[{"name":"CONICET Universidad de Buenos Aires Instituto de Qu\u00edmica Biol\u00f3gica de la Facultad de Ciencias Exactas y Naturales (IQUIBICEN) Buenos Aires Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elizabeth","family":"Soczewski","sequence":"additional","affiliation":[{"name":"CONICET Universidad de Buenos Aires Instituto de Qu\u00edmica Biol\u00f3gica de la Facultad de Ciencias Exactas y Naturales (IQUIBICEN) Buenos Aires Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Soledad","family":"Gori","sequence":"additional","affiliation":[{"name":"CONICET Universidad de Buenos Aires Instituto de Qu\u00edmica Biol\u00f3gica de la Facultad de Ciencias Exactas y Naturales (IQUIBICEN) Buenos Aires Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guillermina","family":"Calo","sequence":"additional","affiliation":[{"name":"CONICET Universidad de Buenos Aires Instituto de Qu\u00edmica Biol\u00f3gica de la Facultad de Ciencias Exactas y Naturales (IQUIBICEN) Buenos Aires Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vanesa","family":"Hauk","sequence":"additional","affiliation":[{"name":"CONICET Universidad de Buenos Aires Instituto de Qu\u00edmica Biol\u00f3gica de la Facultad de Ciencias Exactas y Naturales (IQUIBICEN) Buenos Aires Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Florencia","family":"Sabbione","sequence":"additional","affiliation":[{"name":"Instituto de Medicina Experimental (IMEX) CONICET Academia Nacional de Medicina Buenos Aires Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lucila","family":"Gallino","sequence":"additional","affiliation":[{"name":"CONICET Universidad de Buenos Aires Instituto de Qu\u00edmica Biol\u00f3gica de la Facultad de Ciencias Exactas y Naturales (IQUIBICEN) Buenos Aires Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gustavo","family":"Mart\u00ednez","sequence":"additional","affiliation":[{"name":"Fertilis Medicina Reproductiva. 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