{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,2]],"date-time":"2026-07-02T07:51:10Z","timestamp":1782978670046,"version":"3.54.5"},"reference-count":48,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2021,5,6]],"date-time":"2021-05-06T00:00:00Z","timestamp":1620259200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001659","name":"Deutsche Forschungsgemeinschaft","doi-asserted-by":"publisher","award":["GE\u20102223\/2\u20101"],"award-info":[{"award-number":["GE\u20102223\/2\u20101"]}],"id":[{"id":"10.13039\/501100001659","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["American J Rep Immunol"],"published-print":{"date-parts":[[2021,9]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>B7\u2010H4, a checkpoint molecule of the B7 family, regulates a broad spectrum such as T\u2010cell activation, cytokine secretion, tumour progression, and invasion capacities. Our previous data revealed that soluble B7\u2010H4 (sB7\u2010H4) blood serum levels are elevated in women at high risk for the hypertensive pregnancy disorder preeclampsia (PE) in the first trimester, as well as in patients with confirmed early\/late\u2010onset PE.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Aim<\/jats:title>\n                    <jats:p>We here aim to investigate the expression pattern of B7\u2010H4 in placental tissues of PE and HELLP Syndrome versus control group.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>B7\u2010H4 protein expression and localization were investigated by immunoblotting and co\u2010immunohistochemistry in placental chorionic villous and decidual basalis tissues.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>B7\u2010H4 protein was prominently expressed at the cell membrane, in the cytoplasm of the syncytiotrophoblast (STB) and interstitial extravillous trophoblast (EVT). B7\u2010H4 protein levels in placental chorionic villous tissue were significantly higher in women with early\u2010onset\/late\u2010onset PE and HELLP, while it was decreased in decidual basalis tissues of early\u2010onset PE and HELLP compared with controls.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>B7\u2010H4 was inversely expressed in placental chorionic villous and decidual basalis tissues of PE and HELLP patients. The increase in B7\u2010H4 in the STB in PE and HELLP may lead to excessive apical expression and release of soluble B7\u2010H4 in the maternal circulation. In contrast, the decrease in B7\u2010H4 in decidual basalis tissues could be related to the decrease in invasion ability of the EVT in PE. Thus, the current results strongly suggest that B7\u2010H4 is involved in the pathogenesis of PE and HELLP.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/aji.13430","type":"journal-article","created":{"date-parts":[[2021,4,17]],"date-time":"2021-04-17T13:00:07Z","timestamp":1618664407000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":11,"title":["Abnormal expression of the costimulatory molecule B7\u2010H4 in placental chorionic villous and decidual basalis tissues of patients with preeclampsia and HELLP syndrome"],"prefix":"10.1111","volume":"86","author":[{"given":"Liyan","family":"Duan","sequence":"first","affiliation":[{"name":"Department of Gynecology and Obstetrics University of Duisburg\u2010Essen Essen Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Beatrix","family":"Reisch","sequence":"additional","affiliation":[{"name":"Department of Gynecology and Obstetrics University of Duisburg\u2010Essen Essen Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Antonella","family":"Iannaccone","sequence":"additional","affiliation":[{"name":"Department of Gynecology and Obstetrics University of Duisburg\u2010Essen Essen Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elina","family":"Hadrovic","sequence":"additional","affiliation":[{"name":"Department of Gynecology and Obstetrics University of Duisburg\u2010Essen Essen Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuqing","family":"Wu","sequence":"additional","affiliation":[{"name":"Institute of Molecular Biology University of Duisburg\u2010Essen Essen Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rebekka","family":"Vogtmann","sequence":"additional","affiliation":[{"name":"Department of Gynecology and Obstetrics University of Duisburg\u2010Essen Essen Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elke","family":"Winterhager","sequence":"additional","affiliation":[{"name":"EMU\/IMCES University of Duisburg\u2010Essen Essen Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rainer","family":"Kimmig","sequence":"additional","affiliation":[{"name":"Department of Gynecology and Obstetrics University of Duisburg\u2010Essen Essen Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Angela","family":"K\u00f6ninger","sequence":"additional","affiliation":[{"name":"Department of Gynecology and Obstetrics University of Duisburg\u2010Essen Essen Germany"},{"name":"Department of Gynecology and Obstetrics Clinic of the Order of St. John St. Hedwigs Clinic Regensburg Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7301-6746","authenticated-orcid":false,"given":"Pawel","family":"Mach","sequence":"additional","affiliation":[{"name":"Department of Gynecology and Obstetrics University of Duisburg\u2010Essen Essen Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alexandra","family":"Gellhaus","sequence":"additional","affiliation":[{"name":"Department of Gynecology and Obstetrics University of Duisburg\u2010Essen Essen Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2021,5,6]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.1800058"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2018.02630"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2020.01082"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.aaw1300"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1080\/01443610903061751"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1111\/aji.12097"},{"key":"e_1_2_8_8_1","volume-title":"HELLP Syndrome","author":"Khalid F","year":"2020"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(05)17987-2"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ejogrb.2013.05.005"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1136\/bmj.39335.385301.BE"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1161\/HYPERTENSIONAHA.114.04850"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1093\/aje\/155.3.203"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jri.2019.07.004"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1155\/2019\/4108271"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ejogrb.2012.09.026"},{"key":"e_1_2_8_17_1","first-page":"389","article-title":"HELLP syndrome and placental inflammatory pathology","volume":"60","author":"Landi B","year":"2008","journal-title":"Minerva Ginecol"},{"key":"e_1_2_8_18_1","doi-asserted-by":"publisher","DOI":"10.1111\/aji.12380"},{"key":"e_1_2_8_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jri.2019.102613"},{"key":"e_1_2_8_20_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2020.00667"},{"key":"e_1_2_8_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1074-7613(03)00152-3"},{"key":"e_1_2_8_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1074-7613(03)00147-X"},{"key":"e_1_2_8_23_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1434299100"},{"key":"e_1_2_8_24_1","doi-asserted-by":"publisher","DOI":"10.1158\/1078-0432.CCR-15-2440"},{"key":"e_1_2_8_25_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2567.2009.03111.x"},{"key":"e_1_2_8_26_1","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.00755-06"},{"key":"e_1_2_8_27_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pmed.1000166"},{"key":"e_1_2_8_28_1","doi-asserted-by":"publisher","DOI":"10.1111\/aji.12988"},{"key":"e_1_2_8_29_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0897.2008.00674.x"},{"key":"e_1_2_8_30_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jri.2013.04.004"},{"key":"e_1_2_8_31_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.placenta.2017.06.004"},{"key":"e_1_2_8_32_1","article-title":"Human placenta and trophoblast development: key molecular mechanisms and model systems","volume":"1","author":"Kn\u00f6fler M","year":"2019","journal-title":"Cell Mol Life Sci"},{"key":"e_1_2_8_33_1","first-page":"e1","article-title":"ACOG Practice Bulletin No. 202. American College of Obstetricians and Gynecologists","volume":"133","author":"Hypertension G. Preeclampsia","year":"2019","journal-title":"Obstet Gynecol"},{"key":"e_1_2_8_34_1","doi-asserted-by":"publisher","DOI":"10.1161\/HYPERTENSIONAHA.117.10803"},{"key":"e_1_2_8_35_1","doi-asserted-by":"publisher","DOI":"10.1002\/uog.14799"},{"key":"e_1_2_8_36_1","article-title":"Isolation of leukocytes from the human maternal\u2010fetal interface","volume":"99","author":"Xu Y","year":"2015","journal-title":"J Vis Exp"},{"key":"e_1_2_8_37_1","doi-asserted-by":"publisher","DOI":"10.1093\/molehr\/gal044"},{"key":"e_1_2_8_38_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.placenta.2009.12.022"},{"key":"e_1_2_8_39_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0198-8859(00)00193-2"},{"key":"e_1_2_8_40_1","doi-asserted-by":"publisher","DOI":"10.3389\/fphar.2014.00288"},{"key":"e_1_2_8_41_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0002-9378(99)70239-5"},{"key":"e_1_2_8_42_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.0901154"},{"key":"e_1_2_8_43_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0188250"},{"key":"e_1_2_8_44_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.171.9.4650"},{"key":"e_1_2_8_45_1","doi-asserted-by":"publisher","DOI":"10.1166\/jbn.2019.2741"},{"key":"e_1_2_8_46_1","doi-asserted-by":"publisher","DOI":"10.1111\/aji.12527"},{"key":"e_1_2_8_47_1","doi-asserted-by":"publisher","DOI":"10.1095\/biolreprod66.2.445"},{"key":"e_1_2_8_48_1","doi-asserted-by":"publisher","DOI":"10.1093\/molehr\/gar067"},{"key":"e_1_2_8_49_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(10)60279-6"}],"container-title":["American Journal of Reproductive Immunology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/aji.13430","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/aji.13430","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/aji.13430","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,11,27]],"date-time":"2025-11-27T15:50:32Z","timestamp":1764258632000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/aji.13430"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,5,6]]},"references-count":48,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2021,9]]}},"alternative-id":["10.1111\/aji.13430"],"URL":"https:\/\/doi.org\/10.1111\/aji.13430","archive":["Portico"],"relation":{},"ISSN":["1046-7408","1600-0897"],"issn-type":[{"value":"1046-7408","type":"print"},{"value":"1600-0897","type":"electronic"}],"subject":[],"published":{"date-parts":[[2021,5,6]]},"assertion":[{"value":"2021-02-12","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2021-04-12","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2021-05-06","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e13430"}}