{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,15]],"date-time":"2026-07-15T03:13:33Z","timestamp":1784085213725,"version":"3.55.0"},"reference-count":33,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2021,7,22]],"date-time":"2021-07-22T00:00:00Z","timestamp":1626912000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["American J Rep Immunol"],"published-print":{"date-parts":[[2021,11]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Preeclampsia (PE) is a serious pregnancy complication. It has been shown that insufficient infiltration of extravillous trophoblasts (EVTs) is related to the pathogenesis of PE. Circular hsa_circ_0001326 (circ_0001326) has been uncovered to be upregulated in PE. However, the influence of circ_0001326 on the infiltration of trophoblasts is indistinct.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>48 pregnant women (25 PE patients and 23 healthy controls) were recruited for this study. Human trophoblasts HTR\u20108\/Svneo were used for function analysis. Expression of circ_0001326 was evaluated by real\u2010time quantitative polymerase chain reaction (RT\u2010qPCR). The diagnostic value of circ_0001326 was analyzed by receiver operating characteristic (ROC) curve. Cell proliferation, migration, and invasion of HTR\u20108\/Svneo cells were determined using 3\u2010(4,5\u2010Dimethylthiazol\u20102\u2010yl)\u20102,5\u2010Diphenyltetrazolium Bromide (MTT), 5\u2010ethynyl\u20102\u2019\u2010deoxyuridine (EdU), wound\u2010healing, and transwell assays. Protein levels were detected using Western blotting. The regulation mechanism of circ_0001326 was surveyed by bioinformatics analysis and dual\u2010luciferase reporter assay.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Circ_0001326 was highly expressed in plasma samples and placental tissues of PE patients. Moreover, plasma circ_0001326 could distinguish PE patients from healthy controls (area under the curve (AUC) =0.793). Functionally, circ_0001326 overexpression repressed HTR\u20108\/Svneo cell proliferation, epithelial\u2010mesenchymal transition (EMT), migration, and invasion, but circ_0001326 silencing had the opposing impact. Mechanically, circ_0001326 regulated interleukin\u201016 (IL16) expression via sponging microRNA (miR)\u2010558. MiR\u2010558 mimic or IL16\u00a0knockdown overturned the inhibiting effect of circ_0001326 overexpression on HTR\u20108\/Svneo cell proliferation, EMT, migration, and invasion.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Circ_0001326 elevated IL16 expression via sponging miR\u2010558, thus curbing HTR\u20108\/Svneo cell proliferation, EMT, migration, and invasion, suggesting that circ_0001326 might be involved in the pathogenesis of PE.<\/jats:p><\/jats:sec>","DOI":"10.1111\/aji.13484","type":"journal-article","created":{"date-parts":[[2021,7,16]],"date-time":"2021-07-16T18:33:27Z","timestamp":1626460407000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":11,"title":["Hsa_circ_0001326 regulates proliferation, migration, invasion, and EMT of HTR\u20108\/SVneo cells via increasing IL16 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