{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T00:43:33Z","timestamp":1787013813425,"version":"build-2736575974"},"reference-count":32,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2021,8,22]],"date-time":"2021-08-22T00:00:00Z","timestamp":1629590400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["American J Rep Immunol"],"published-print":{"date-parts":[[2021,12]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Problem<\/jats:title>\n                    <jats:p>Proper placental development is pivotal to ensure healthy pregnancy outcomes. Among the multiple cellular mechanisms involved in the orchestration of this process, little is known on the role of alternative splicing events in the modulation of trophoblast cell biology. Here, we evaluated the expression of the alternative splicing regulator Rbfox2 in the pre\u2010 and post\u2010placentation period in mouse pregnancies in both healthy and pathological settings.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Method of study<\/jats:title>\n                    <jats:p>Immunofluorescence analysis of Rbfox2 expression in mouse implantation sites collected during the pre\u2010placentation period (E5\u2010E7) and post\u2010placentation (E13).<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>We identified a progressive increase of Rbfox2 levels throughout the peri\u2010implantation period with a shift from a cytoplasmatic expression on E5\u2010E6 to a predominantly nuclear expression on E7, together with a prominent expression of this factor in both subcellular compartments of the primitive placenta. Our results further showed that in contrast to healthy gestations, Rbfox2 expression decreased in preeclamptic models during the post\u2010placentation period. Finally, we further demonstrated enhanced expression of Rbfox2 proteins in allogeneic pregnancy compared to syngeneic models.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>Our findings uncover a novel role for Rbfox2\u2010controlled splicing events in the modulation of trophoblast function, with potential implications for the pathogenesis of preeclampsia and other pregnancy complications originated from defective placentation.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/aji.13491","type":"journal-article","created":{"date-parts":[[2021,8,7]],"date-time":"2021-08-07T07:10:45Z","timestamp":1628320245000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Expression of the alternative splicing regulator Rbfox2 during placental development is differentially regulated in preeclampsia mouse models"],"prefix":"10.1111","volume":"86","author":[{"given":"Nancy","family":"Freitag","sequence":"first","affiliation":[{"name":"Department of Obstetrics and Fetal Medicine University Medical Center Hamburg\u2010Eppendorf  Hamburg Germany"},{"name":"Experimental and Clinical Research Center a Cooperation between the Max Delbr\u00fcck Center for Molecular Medicine in the Helmholtz Association AG GlycoImmunology and the Charit\u00e9 \u2010 Universit\u00e4tsmedizin Berlin  Berlin Germany"},{"name":"Department for Psychosomatic Medicine Charit\u00e9 \u2013 Universit\u00e4tsmedizin Berlin, Humboldt\u2010Universit\u00e4t zu Berlin and Berlin Institute of Health  Berlin Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yiran","family":"Xie","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Fetal Medicine University Medical Center Hamburg\u2010Eppendorf  Hamburg Germany"},{"name":"Reproductive Medicine Center Taihe Hospital Hubei Medical University  Shiyan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lisa\u2010Marie","family":"Adam","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Fetal Medicine University Medical Center Hamburg\u2010Eppendorf  Hamburg Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sophia","family":"Borowski","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Fetal Medicine University Medical Center Hamburg\u2010Eppendorf  Hamburg Germany"},{"name":"Experimental and Clinical Research Center a Cooperation between the Max Delbr\u00fcck Center for Molecular Medicine in the Helmholtz Association AG GlycoImmunology and the Charit\u00e9 \u2010 Universit\u00e4tsmedizin Berlin  Berlin Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0434-2660","authenticated-orcid":false,"given":"Sandra 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