{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,28]],"date-time":"2026-08-28T00:36:33Z","timestamp":1787877393629,"version":"build-2784847793"},"reference-count":51,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2021,9,17]],"date-time":"2021-09-17T00:00:00Z","timestamp":1631836800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81901568"],"award-info":[{"award-number":["81901568"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["American J Rep Immunol"],"published-print":{"date-parts":[[2021,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The disruption of the inflammatory microenvironment in the uterus affects pregnancy outcome. However, the exact quantification and distribution of leukocyte subpopulations in the uterus in preeclampsia (PE) have not been clearly characterized. Inflammasomes promote the release of proinflammatory cytokines interleukin (IL)\u2010\u03b2 and IL\u201018. A higher expression of NLRP3 inflammasome in placentas contributes to excessive inflammation in PE. However, related studies on the uterus are scarce. We aimed to investigate changes in the infiltration of leukocyte subpopulations in decidual and uterine tissues, and explore the role of activation of uterine NLRP3 inflammasomes in PE. Decidual tissues were collected from normotensive pregnant women and preeclamptic women. A PE\u2010like model was established via administration of lipopolysaccharide to normal pregnant rats. Uterine and decidual tissues were collected from all experimental groups. It was found that the number of leukocytes was significantly elevated in decidual and uterine tissues in PE patients compared to normal controls. The leukocytes (predominantly macrophages and NK cells) particularly infiltrated into the decidua and uterine decidua in PE\u2010like rats, and these were sparse in the myometrium. The NLRP3 immunoreactivity in the uterus was extremely little in control rats, its immunoreactivity and caspase\u20101 immunoreactivity were significantly elevated in the PE\u2010like rats; the mRNA expression results also indicated an upward trend in the activation of NLRP3 inflammasomes. These results support that leucocyte infiltration in the decidua and uterine deciduas, and the activation of NLRP3 inflammasome in the uterus, which participate in the pathogenesis, are responsible for the excessive inflammation at the maternal\u2010fetal interface during PE.<\/jats:p>","DOI":"10.1111\/aji.13493","type":"journal-article","created":{"date-parts":[[2021,8,10]],"date-time":"2021-08-10T15:40:01Z","timestamp":1628610001000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":15,"title":["Increased uterine NLRP3 inflammasome and leucocyte infiltration in a rat model of preeclampsia"],"prefix":"10.1111","volume":"86","author":[{"given":"Huiqian","family":"Zeng","sequence":"first","affiliation":[{"name":"Department of Obstetrics and Gynecology Guangzhou Women and Children's Medical Center Guangzhou Medical University  Guangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xinjia","family":"Han","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology Guangzhou Women and Children's Medical Center Guangzhou Medical University  Guangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhiqin","family":"Zhu","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology Guangzhou Women and Children's Medical Center Guangzhou Medical University  Guangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shengjun","family":"Yu","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology Guangzhou Women and Children's Medical Center Guangzhou Medical University  Guangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shanshan","family":"Mei","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology Guangzhou Women and Children's Medical Center Guangzhou Medical University  Guangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xi","family":"Cheng","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology Guangzhou Women and Children's Medical Center Guangzhou Medical University  Guangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Weiqiang","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology Guangzhou Women and Children's Medical Center Guangzhou Medical University  Guangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guanglan","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology Guangzhou Women and Children's Medical Center Guangzhou Medical University  Guangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dajun","family":"Fang","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology Guangzhou Women and Children's Medical Center Guangzhou Medical University  Guangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2021,9,17]]},"reference":[{"issue":"5","key":"e_1_2_9_2_1","first-page":"1122","article-title":"Hypertension in pregnancy. Report of the American College of Obstetricians and Gynecologists' Task Force on Hypertension in Pregnancy","volume":"122","author":"American College of O, Gynecologists, Task Force on Hypertension in P","year":"2013","journal-title":"Obstet Gynecol"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrneph.2014.102"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbi.2019.04.021"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1038\/hr.2016.53"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2019.01305"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2018.01659"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1189\/jlb.1112603"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2018.03142"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1111\/aji.12509"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1093\/molehr\/gag001"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2010.01.040"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0897.2010.00960.x"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41419-019-2162-4"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jri.2017.09.002"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.3390\/molecules24081548"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2018.02597"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.1095\/biolreprod.111.095505"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.1302891"},{"key":"e_1_2_9_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.fertnstert.2012.08.034"},{"key":"e_1_2_9_21_1","doi-asserted-by":"publisher","DOI":"10.1111\/aji.13153"},{"key":"e_1_2_9_22_1","doi-asserted-by":"publisher","DOI":"10.1093\/molehr\/gaz067"},{"issue":"1","key":"e_1_2_9_23_1","first-page":"159","article-title":"ACOG practice bulletin. Diagnosis and management of preeclampsia and eclampsia. Number 33, January 2002","volume":"99","author":"Bulletins\u2013Obstetrics ACoP","year":"2002","journal-title":"Obstet Gynecol"},{"key":"e_1_2_9_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/0002-9378(94)90463-4"},{"key":"e_1_2_9_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.reprotox.2012.09.009"},{"key":"e_1_2_9_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuroscience.2016.04.011"},{"key":"e_1_2_9_27_1","doi-asserted-by":"publisher","DOI":"10.1590\/1414-431x20198273"},{"key":"e_1_2_9_28_1","doi-asserted-by":"publisher","DOI":"10.1177\/1933719115616497"},{"key":"e_1_2_9_29_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jri.2005.04.005"},{"key":"e_1_2_9_30_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12916-016-0632-4"},{"key":"e_1_2_9_31_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1706546114"},{"key":"e_1_2_9_32_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2018.01291"},{"key":"e_1_2_9_33_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.1801350"},{"key":"e_1_2_9_34_1","doi-asserted-by":"publisher","DOI":"10.3390\/cells9010079"},{"key":"e_1_2_9_35_1","doi-asserted-by":"publisher","DOI":"10.1177\/1933719112459240"},{"key":"e_1_2_9_36_1","doi-asserted-by":"publisher","DOI":"10.1211\/0022357991772853"},{"key":"e_1_2_9_37_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00441-019-03104-9"},{"key":"e_1_2_9_38_1","doi-asserted-by":"publisher","DOI":"10.1111\/cei.13130"},{"key":"e_1_2_9_39_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-065X.2011.01046.x"},{"key":"e_1_2_9_40_1","doi-asserted-by":"publisher","DOI":"10.1111\/nyas.12458"},{"key":"e_1_2_9_41_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.fertnstert.2015.09.027"},{"key":"e_1_2_9_42_1","doi-asserted-by":"publisher","DOI":"10.1111\/aji.12440"},{"key":"e_1_2_9_43_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1097-2765(02)00599-3"},{"key":"e_1_2_9_44_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2006.08.002"},{"key":"e_1_2_9_45_1","doi-asserted-by":"publisher","DOI":"10.1189\/jlb.1206755"},{"key":"e_1_2_9_46_1","doi-asserted-by":"publisher","DOI":"10.3389\/fendo.2020.00080"},{"key":"e_1_2_9_47_1","doi-asserted-by":"publisher","DOI":"10.3390\/cells9071642"},{"key":"e_1_2_9_48_1","doi-asserted-by":"publisher","DOI":"10.1097\/CM9.0000000000001214"},{"key":"e_1_2_9_49_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0165-0378(03)00044-5"},{"key":"e_1_2_9_50_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ajog.2012.06.047"},{"key":"e_1_2_9_51_1","doi-asserted-by":"publisher","DOI":"10.1095\/biolreprod.111.097014"},{"key":"e_1_2_9_52_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1471-0528.2009.02428.x"}],"container-title":["American Journal of Reproductive Immunology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/aji.13493","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/aji.13493","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/aji.13493","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,8,28]],"date-time":"2023-08-28T07:40:51Z","timestamp":1693208451000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/aji.13493"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,9,17]]},"references-count":51,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2021,12]]}},"alternative-id":["10.1111\/aji.13493"],"URL":"https:\/\/doi.org\/10.1111\/aji.13493","archive":["Portico"],"relation":{},"ISSN":["1046-7408","1600-0897"],"issn-type":[{"value":"1046-7408","type":"print"},{"value":"1600-0897","type":"electronic"}],"subject":[],"published":{"date-parts":[[2021,9,17]]},"assertion":[{"value":"2021-05-12","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2021-08-04","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2021-09-17","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e13493"}}