{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T14:08:16Z","timestamp":1787062096411,"version":"build-2736575974"},"reference-count":33,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2021,12,2]],"date-time":"2021-12-02T00:00:00Z","timestamp":1638403200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["American J Rep Immunol"],"published-print":{"date-parts":[[2022,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>Preeclampsia (PE) is a common hypertensive disorder of pregnancy. Recent studies have suggested that circular RNAs (circRNAs) play a pathological role in PE. Herein, this study aimed to investigate the action and mechanism of circ_0037078 in PE process.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>The quantitative real\u2010time PCR (qRT\u2010PCR) and Western blot were used to determine the expression levels of RNAs and genes. Cell proliferation, migration, invasion and angiogenesis were evaluated by using cell counting kit\u20108 (CCK\u20108), colony formation, transwell, and tube formation assays, respectively. The target relation between miR\u2010576\u20105p and IL1RAP (Interleukin\u20101 receptor accessory protein) and circ_0037078 was predicted by bioinformatics analysis and verified by dual\u2010luciferase reporter and RNA immunoprecipitation (RIP) assays.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>Circ_0037078 expression was higher in placental tissues of patients with PE than that of normal control. Knockdown of circ_0037078 led to an enhancement of the proliferation, migration, invasion, and angiogenesis in trophoblast cells. Mechanistically, circ_0037078 acted as a sponge for miR\u2010576\u20105p, thus elevating the expression of IL1RAP, which was targeted by IL1RAP. Further rescue experiments suggested that miR\u2010576\u20105p inhibition reversed the effects of circ_0037078 knockdown on above behaviors of trophoblast cells. Moreover, miR\u2010576\u20105p overexpression enhanced the proliferative, migratory, invasive, angiogenic phenotypes of trophoblast cells, which were attenuated by IL1RAP up\u2010regulation.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>Circ_0037078 knockdown promotes trophoblast cell proliferation, migration, invasion, and angiogenesis in vitro by miR\u2010576\u20105p\/IL1RAP axis, providing a novel insight into the etiology of PE.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/aji.13507","type":"journal-article","created":{"date-parts":[[2021,11,1]],"date-time":"2021-11-01T10:21:26Z","timestamp":1635762086000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":19,"title":["Circ_0037078 promotes trophoblast cell proliferation, migration, invasion and angiogenesis by miR\u2010576\u20105p\/IL1RAP axis"],"prefix":"10.1111","volume":"87","author":[{"given":"Hong","family":"Zou","sequence":"first","affiliation":[{"name":"Department of Gynecology Jingmen First People's Hospital  Jingmen China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qinghua","family":"Mao","sequence":"additional","affiliation":[{"name":"Department of Obstetrics Jingmen First People's Hospital  Jingmen 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