{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T14:50:37Z","timestamp":1787064637477,"version":"build-2736575974"},"reference-count":33,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2022,2,16]],"date-time":"2022-02-16T00:00:00Z","timestamp":1644969600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["American J Rep Immunol"],"published-print":{"date-parts":[[2022,4]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Problem<\/jats:title>\n                    <jats:p>Systemic inflammation induced by infection, which is associated with testicular inflammation, predisposes males to subfertility. Recently, the nucleotide\u2010binding oligomerization domain, leucine\u2010rich repeat\u2010, and pyrin domain\u2010containing 3 (NLRP3) inflammasome was identified as a key mediator of inflammation, and excessive activation of the NLRP3 inflammasome was shown to contribute to the pathogenesis of a wide variety of diseases. However, the mechanisms underlying infectious inflammation in the testis remain unclear. We investigated the effect of lipopolysaccharide (LPS)\u2010induced systemic inflammation on the role of the NLRP3 inflammasome in murine testes.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Method of study<\/jats:title>\n                    <jats:p>We performed in vivo and in vitro studies using an LPS\u2010induced model of NLRP3 inflammasome activation and testicular inflammation.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>Intraperitoneal administration of LPS significantly impaired sperm motility in the epididymis of wild type (WT) and NLRP3\u2010knockout (KO) mice. LPS administration stimulated interleukin (IL)\u20101\u03b2 production and secretion in the testes of WT mice, and these adverse effects were improved in the testes of NLRP3\u2010KO mice. LPS administration also stimulated neutrophil infiltration as well as its chemoattractant C\u2010C motif chemokine ligand 2 (CCL2) in WT testes, which were suppressed in NLRP3\u2010KO testes. In in vitro cell culture, treatment with LPS and NLRP3 inflammasome activation significantly induced IL\u20101\u03b2 and CCL2 secretion from WT but not NLRP3\u2010KO testicular cells.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>Taken together, our results suggest that testicular cells have the potential to secrete IL\u20101\u03b2 and CCL2 in an NLRP3 inflammasome\u2010dependent manner and that these cytokines from the testis may further exacerbate testicular function, resulting in subfertility during infectious diseases.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/aji.13527","type":"journal-article","created":{"date-parts":[[2022,2,11]],"date-time":"2022-02-11T09:54:42Z","timestamp":1644573282000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":31,"title":["NLRP3 inflammasome is involved in testicular inflammation induced by lipopolysaccharide in mice"],"prefix":"10.1111","volume":"87","author":[{"given":"Michiya","family":"Sano","sequence":"first","affiliation":[{"name":"Laboratory of Animal Reproduction Department of Agriculture Tokyo University of Agriculture  Atsugi Kanagawa Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hiromu","family":"Komiyama","sequence":"additional","affiliation":[{"name":"Laboratory of Animal Reproduction Department of Agriculture Tokyo University of Agriculture  Atsugi Kanagawa Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Riina","family":"Shinoda","sequence":"additional","affiliation":[{"name":"Laboratory of Animal Reproduction Department of Agriculture Tokyo University of Agriculture  Atsugi Kanagawa Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ren","family":"Ozawa","sequence":"additional","affiliation":[{"name":"Laboratory of Animal Reproduction Department of Agriculture Tokyo University of Agriculture  Atsugi Kanagawa Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hiroyuki","family":"Watanabe","sequence":"additional","affiliation":[{"name":"Department of Life and Food Sciences Obihiro University of Agriculture and Veterinary Medicine  Obihiro Hokkaido Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tadayoshi","family":"Karasawa","sequence":"additional","affiliation":[{"name":"Division of Inflammation Research Center for Molecular Medicine Jichi Medical University  Shimotsuke Tochigi Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Masafumi","family":"Takahashi","sequence":"additional","affiliation":[{"name":"Division of Inflammation Research Center for Molecular Medicine Jichi Medical University  Shimotsuke Tochigi Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yasushi","family":"Torii","sequence":"additional","affiliation":[{"name":"Laboratory of 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