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The pathogenesis of pre\u2010eclampsia remains incompletely understood. The role of peripheral natural killer cells (NK cells) in the pre\u2010eclampsia is unclear.<\/jats:p><\/jats:sec><jats:sec><jats:title>Method of study<\/jats:title><jats:p>Flow cytometry was performed to detect the expression of CD158a (KIR2DL1) and CD158b (KIR2DL2\/3) in peripheral NK cells of healthy pregnant women (HP) and patients with pre\u2010eclampsia (PE). Differentially expressed genes (DEGs) in CD158a<jats:sup>+<\/jats:sup> and CD158b<jats:sup>+<\/jats:sup> NK cells were identified by RNA\u2010sequencing and real\u2010time PCR. Protein array analysis was used to identify altered protein levels in the serum of study participants.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>CD158a<jats:sup>+<\/jats:sup> NK cell numbers were increased in the peripheral blood of patients while the number of CD158b<jats:sup>+<\/jats:sup> NK cells was reduced. In addition, the percentage of CD158a<jats:sup>+<\/jats:sup> NK cells within the peripheral NK subset was positively correlated with systolic blood pressure while the percentage of CD158b<jats:sup>+<\/jats:sup> NK cells was negatively correlated with systolic blood pressure. RNA\u2010seq and real\u2010time PCR showed that the expression of <jats:italic>ERAP2<\/jats:italic> and <jats:italic>GCH1<\/jats:italic>, the genes that regulate blood pressure and angiogenesis, was decreased in CD158a<jats:sup>+<\/jats:sup> compared to CD158b<jats:sup>+<\/jats:sup> NK cells. Consistently, the level of proteins involved in angiogenesis was altered in the serum of pre\u2010eclampsia patients compared to healthy individuals.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>CD158a<jats:sup>+<\/jats:sup> NK cells increased while CD158b<jats:sup>+<\/jats:sup> NK cells decreased in the peripheral blood of patients with pre\u2010eclampsia compared to healthy individuals. The change in the frequency of CD158a<jats:sup>+<\/jats:sup>\/CD158b<jats:sup>+<\/jats:sup> NK cells is related to the increase in blood pressure.<\/jats:p><\/jats:sec>","DOI":"10.1111\/aji.13532","type":"journal-article","created":{"date-parts":[[2022,3,7]],"date-time":"2022-03-07T09:50:35Z","timestamp":1646646635000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["CD158a<sup>+<\/sup>\/CD158b<sup>+<\/sup> NK cell imbalance correlates with hypertension in patients with pre\u2010eclampsia"],"prefix":"10.1111","volume":"87","author":[{"given":"Xiuxiu","family":"Xu","sequence":"first","affiliation":[{"name":"Institute of Gerontology, The First Affiliated Hospital of USTC, Division of Life Sciences and Medicine University of Science and Technology of China  Hefei Anhui P.R. China"},{"name":"Institute of Immunology University of Science and Technology of China  Hefei Anhui P.R. 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