{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,21]],"date-time":"2026-02-21T04:50:21Z","timestamp":1771649421666,"version":"3.50.1"},"reference-count":39,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2022,4,11]],"date-time":"2022-04-11T00:00:00Z","timestamp":1649635200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["American J Rep Immunol"],"published-print":{"date-parts":[[2022,8]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Problem<\/jats:title><jats:p>To investigate how asymptomatic bacterial imbalance affects the clinical pregnancy rate after artificial insemination with the husband's semen (AIH).<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>This study included married heterosexual couples who underwent AIH. According to the follow\u2010up results, participants were divided into the pregnancy and non\u2010pregnancy groups. Based on the first 10 pair participants in each group with vaginal flora bacterial 16S rRNA sequencing results, six semen samples received bacterial\u2010sperm mixed test. Moreover, 34 cytokines were detected in the peripheral blood sera of the first three pairs by high\u2010throughput Luminex, which were verified in vaginal secretions, cervical mucus, and blood sera from the first 200 pairs by ELISA.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>The results of the 16S sequencing of vaginal secretions showed that compared with the pregnant group, the non\u2010pregnant group had a significantly increased bacterial species diversity, which was mainly manifested by a decrease in <jats:italic>Lactobacillus crispatus<\/jats:italic> and an increase in <jats:italic>Prevotella bivia<\/jats:italic>. When <jats:italic>Prevotella bivia<\/jats:italic> or <jats:italic>Lactobacillus crispatus<\/jats:italic> were mixed with sperms, the sperm motility was decreased (<jats:italic>p<\/jats:italic>\u00a0&lt;\u00a0.05). The vaginal posterior fornix secretions, cervical mucus, and peripheral blood sera of the non\u2010pregnant group showed decreased levels of MIP\u20101\u03b1 and increased levels of IL\u201017A (<jats:italic>p<\/jats:italic>\u00a0&lt;\u00a0.05).<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>The imbalance of vaginal flora leading to the increase of <jats:italic>Prevotella bivia<\/jats:italic> and the decrease of <jats:italic>Lactobacillus crispatus<\/jats:italic> may cause an imbalance of immune regulation. Low expression of MIP\u20101\u03b1 and high expression of IL\u201017A were associated with reduced clinical pregnancy rate in AIH.<\/jats:p><\/jats:sec>","DOI":"10.1111\/aji.13543","type":"journal-article","created":{"date-parts":[[2022,3,31]],"date-time":"2022-03-31T09:13:31Z","timestamp":1648718011000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Associations between vaginal flora, MIP\u20101\u03b1, IL\u201017A, and clinical pregnancy rate in AIH"],"prefix":"10.1111","volume":"88","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-0252-9675","authenticated-orcid":false,"given":"Hui","family":"Yang","sequence":"first","affiliation":[{"name":"Department of Gynecology and Obstetrics The First Affiliated Hospital of Soochow University  Suzhou Jiangsu P.R. China"},{"name":"Department of Gynecology and Obstetrics Huaian Maternal and Child Health Care Hospital  Huai'an Jiangsu P.R. China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chengzhen","family":"Gao","sequence":"additional","affiliation":[{"name":"Department of Gynecology and Obstetrics The First Affiliated Hospital of Soochow University  Suzhou Jiangsu P.R. China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xia","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Gynecology and Obstetrics The First Affiliated Hospital of Soochow University  Suzhou Jiangsu P.R. China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fenglong","family":"Qiu","sequence":"additional","affiliation":[{"name":"Department of Gynecology and Obstetrics Huaian Maternal and Child Health Care Hospital  Huai'an Jiangsu P.R. China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mian","family":"Wei","sequence":"additional","affiliation":[{"name":"Department of Gynecology and Obstetrics Huaian Maternal and Child Health Care Hospital  Huai'an Jiangsu P.R. China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5306-2892","authenticated-orcid":false,"given":"Fei","family":"Xia","sequence":"additional","affiliation":[{"name":"Department of Gynecology and Obstetrics The First Affiliated Hospital of Soochow University  Suzhou Jiangsu P.R. China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2022,4,11]]},"reference":[{"key":"e_1_2_9_2_1","first-page":"1862","article-title":"Relationship between infertility\u2010related stress and resilience with posttraumatic growth in infertile couples: gender differences and dyadic interaction","volume":"36","author":"Zhang X","year":"2021","journal-title":"Hum Reprod (Oxford)"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.3390\/jcm9123899"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1093\/humrep\/dez245"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jiph.2020.05.024"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1177\/1753495X18775899"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.2478\/ii-2018-0007"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12866-021-02094-5"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10815-020-01878-4"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.21037\/tau-20-1385"},{"key":"e_1_2_9_11_1","first-page":"794","article-title":"Female causes of infertility are associated with higher risk of preterm birth and low birth weight: analysis of 117 401 singleton live births following IVF","volume":"36","author":"Sunkara SK","year":"2020","journal-title":"Hum Reprod (Oxford)"},{"issue":"2","key":"e_1_2_9_12_1","article-title":"Diagnosis and treatment of male infertility","volume":"183","author":"Fode M","year":"2021","journal-title":"Ugeskr Laeger"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.rbmo.2014.10.002"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1080\/19396368.2018.1453563"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.5582\/bst.2018.01164"},{"issue":"2","key":"e_1_2_9_16_1","first-page":"85","article-title":"The influence of antibiotics and dietary components on gut microbiota","volume":"13","author":"Dudek\u2010Wicher RK","year":"2018","journal-title":"Prz Gastroenterol"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.1111\/aji.13438"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms20143405"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.3390\/pathogens10020090"},{"key":"e_1_2_9_20_1","first-page":"4240","article-title":"Characterisation of probiotic properties of lactic acid bacteria isolated from vaginal microflora of healthy Turkish women","volume":"29","author":"Kiray E","year":"2020","journal-title":"Fresenius Environ Bull"},{"key":"e_1_2_9_21_1","doi-asserted-by":"publisher","DOI":"10.1111\/1471\u20100528.15854"},{"key":"e_1_2_9_22_1","doi-asserted-by":"publisher","DOI":"10.1136\/bmjopen-2017-019301"},{"key":"e_1_2_9_23_1","doi-asserted-by":"publisher","DOI":"10.1111\/imm.12760"},{"key":"e_1_2_9_24_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2019.00462"},{"key":"e_1_2_9_25_1","doi-asserted-by":"publisher","DOI":"10.3390\/microorganisms9010039"},{"key":"e_1_2_9_26_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-020-75534-3"},{"key":"e_1_2_9_27_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10096-020-03862-3"},{"key":"e_1_2_9_28_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ajog.2020.10.019"},{"key":"e_1_2_9_29_1","doi-asserted-by":"publisher","DOI":"10.1093\/infdis\/jiy704"},{"key":"e_1_2_9_30_1","doi-asserted-by":"publisher","DOI":"10.1002\/ajhb.23196"},{"key":"e_1_2_9_31_1","doi-asserted-by":"publisher","DOI":"10.1097\/MD.0000000000023558"},{"key":"e_1_2_9_32_1","doi-asserted-by":"publisher","DOI":"10.1111\/jcpe.13423"},{"key":"e_1_2_9_33_1","doi-asserted-by":"publisher","DOI":"10.1111\/aji.13241"},{"key":"e_1_2_9_34_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.scitotenv.2019.05.041"},{"key":"e_1_2_9_35_1","doi-asserted-by":"publisher","DOI":"10.5468\/ogs.19131"},{"key":"e_1_2_9_36_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrc.2018.12.103"},{"key":"e_1_2_9_37_1","doi-asserted-by":"publisher","DOI":"10.1111\/aji.12435"},{"key":"e_1_2_9_38_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.1601551"},{"key":"e_1_2_9_39_1","doi-asserted-by":"publisher","DOI":"10.1080\/08923973.2020.1835951"},{"key":"e_1_2_9_40_1","doi-asserted-by":"publisher","DOI":"10.1080\/09513590.2018.1459546"}],"container-title":["American Journal of Reproductive Immunology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/aji.13543","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/aji.13543","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/aji.13543","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,8,24]],"date-time":"2023-08-24T08:54:38Z","timestamp":1692867278000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/aji.13543"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,4,11]]},"references-count":39,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2022,8]]}},"alternative-id":["10.1111\/aji.13543"],"URL":"https:\/\/doi.org\/10.1111\/aji.13543","archive":["Portico"],"relation":{},"ISSN":["1046-7408","1600-0897"],"issn-type":[{"value":"1046-7408","type":"print"},{"value":"1600-0897","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,4,11]]},"assertion":[{"value":"2021-11-15","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2022-03-23","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2022-04-11","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e13543"}}