{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,9]],"date-time":"2026-01-09T23:29:47Z","timestamp":1768001387953,"version":"3.49.0"},"reference-count":65,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2022,7,10]],"date-time":"2022-07-10T00:00:00Z","timestamp":1657411200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["American J Rep Immunol"],"published-print":{"date-parts":[[2022,9]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>We previously reported that interferon\u2010tau (IFNT), derived from day\u20107 blastocyst, generates anti\u2010inflammatory responses in bovine endometrial epithelial cells (BEECs) in vitro. However, the real in vivo impact of early embryo\u2010derived IFNT on the uterine proteomic profile is mostly unknown. This study aimed to investigate proteomic changes of uterine flush (UF) when infused with a low physiological level of IFNT without embryo on day\u20108 post\u2010estrus and its possible impact on the uterine immunological microenvironment. First, a fresh medium was infused into the uterine lumen on day\u20106, from which UF was obtained 24 h later, and this procedure was repeated on day\u20107 (control UF). On day\u20108, this procedure was done with a medium containing recombinant bovine IFNT (100 pg\/ml) (IFNT\u2010supplemented UF). Control and IFNT\u2010supplemented UF were tested for immune responses in peripheral blood mononuclear cells (PBMCs). Real\u2010time PCR results revealed that IFNT\u2010supplemented UF downregulated pro\u2010inflammatory cytokines (<jats:italic>TNFA, IL1B)<\/jats:italic> and upregulated anti\u2010inflammatory cytokine (<jats:italic>TGFB1)<\/jats:italic> and <jats:italic>PTGES<\/jats:italic> in PBMCs. Through 2\u2010D PAGE, followed by TOF\/TOF mass spectrometer, apolipoprotein\u2010A1 (Apo\u2010A1) protein was identified in the IFNT\u2010supplemented UF, which was confirmed by ELISA analysis. Proteomic analysis revealed again that the in vitro stimulation of BEECs by IFNT upregulated Apo\u2010A1 expression. Further, stimulation of PBMCs with recombinant bovine Apo\u2010A1 downregulated <jats:italic>TNFA<\/jats:italic> and <jats:italic>NFKB<\/jats:italic> and upregulated <jats:italic>TGFB1<\/jats:italic> and <jats:italic>PTGES<\/jats:italic> in PBMCs. Altogether, our results suggest that minute amounts of IFNT alone, normally secreted from bovine blastocyst, stimulate Apo\u2010A1 secretion from the endometrial epithelium in the absence of embryo that initiates an anti\u2010inflammatory environment, which could pave the way for the acceptance of early embryo in the uterus.<\/jats:p>","DOI":"10.1111\/aji.13592","type":"journal-article","created":{"date-parts":[[2022,7,4]],"date-time":"2022-07-04T20:23:45Z","timestamp":1656966225000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Intrauterine infusion of low levels of interferon\u2010tau on day\u20108 post\u2010estrus stimulates the bovine endometrium to secrete apolipoprotein\u2010A1: A possible implication for early embryo tolerance"],"prefix":"10.1111","volume":"88","author":[{"given":"Mohammad B.","family":"Rashid","sequence":"first","affiliation":[{"name":"Global Agromedicine Research Center (GAMRC) Obihiro 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