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The differentially expressed genes (DEGs) were analyzed using the \u201climma\u201d package and then GO, KEGG, GSEA, and GSVA were applied to perform functional and pathway enrichment analysis. The immune cell infiltration in the RIF process was evaluated by the CIBERSORT algorithm. Finally, the hub genes were identified through the CytoHubba and subsequently verified using two items of external endometrial data.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>236 genes were differentially expressed in the endometrium of the RIF group. Functional enrichment analysis demonstrated that the biological functions of DEGs were mainly correlated to the immune\u2010related pathways, including immune response, TNF signaling pathway, complement and coagulation cascades. Among the immune cells, \u03b3\u03b4 T cells decreased significantly in the endometrium of RIF patients. In addition, the key DEGs such as <jats:italic>PTGS2<\/jats:italic>, <jats:italic>FGB<\/jats:italic>, <jats:italic>MUC1<\/jats:italic>, <jats:italic>SST<\/jats:italic>, <jats:italic>VCAM1<\/jats:italic>, <jats:italic>MMP7<\/jats:italic>, <jats:italic>ERBB4<\/jats:italic>, <jats:italic>FOLR1<\/jats:italic>, and <jats:italic>C3<\/jats:italic> were screened and identified as the hub genes involved in the pathogenesis of RIF.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>Abnormal immune response regulation of endometrium contributes to the occurrence of RIF, and \u03b3\u03b4 T cells may be the pivotal immune cells causing RIF. At the same time, the novel hub genes identified will provide effective targets for the prediction and therapy of RIF.<\/jats:p><\/jats:sec>","DOI":"10.1111\/aji.13607","type":"journal-article","created":{"date-parts":[[2022,8,5]],"date-time":"2022-08-05T10:01:14Z","timestamp":1659693674000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":18,"title":["Identification of key genes and immune cell infiltration in recurrent implantation failure: A study based on integrated analysis of multiple microarray studies"],"prefix":"10.1111","volume":"88","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-1087-7685","authenticated-orcid":false,"given":"Xue","family":"Feng","sequence":"first","affiliation":[{"name":"Reproductive Medicine Center Tongji Hospital Tongji Medical College Huazhong University of Science and Technology  Wuhan Hubei 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