{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,7,30]],"date-time":"2025-07-30T12:27:38Z","timestamp":1753878458717,"version":"3.41.2"},"reference-count":42,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2022,10,26]],"date-time":"2022-10-26T00:00:00Z","timestamp":1666742400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100012467","name":"Distinguished Professor of Liaoning Province","doi-asserted-by":"publisher","award":["2017"],"award-info":[{"award-number":["2017"]}],"id":[{"id":"10.13039\/501100012467","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["American J Rep Immunol"],"published-print":{"date-parts":[[2023,6]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Problem<\/jats:title><jats:p>The maternal\u2010fetal immune abnormalities can result in recurrent spontaneous abortion (RSA). The role of NKG2C<jats:sup>+<\/jats:sup> and LILRB1<jats:sup>+<\/jats:sup> pNK subsets in predicting pregnancy loss is uncertain.<\/jats:p><\/jats:sec><jats:sec><jats:title>Method of Study<\/jats:title><jats:p>In this study, we aimed to compare the percentage of CD3<jats:sup>\u2212<\/jats:sup>CD56<jats:sup>+<\/jats:sup> NK cells, NKG2C<jats:sup>+<\/jats:sup> NK cells, and LILRB1<jats:sup>+<\/jats:sup> NK cells in peripheral blood between healthy pregnant women (HC group), RSA women followed by normal pregnancy (RSA\u2010N group) and RSA women followed by abortion (RSA\u2010A group) in the first trimester via flow cytometry, and explore the prediction value of NKG2C<jats:sup>+<\/jats:sup> and LILRB1<jats:sup>+<\/jats:sup> NK cells for pregnancy loss in RSA via ROC curve. The MFI of NKG2C and LILRB1 of dNK were compared between and HC and RSA\u2010A groups.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>The percentage of CD3\u2010CD56<jats:sup>+<\/jats:sup> pNK cells between HC, RSA\u2010N, and RSA\u2010A groups shows no significant difference. In peripheral blood, the percentage of NKG2C<jats:sup>+<\/jats:sup> NK cells were significantly increased in the RSA\u2010A group than HC group and RSA\u2010N group, and the percentage of LILRB1<jats:sup>+<\/jats:sup> NK cell were significantly decreased in the RSA\u2010A group. The MFI of NKG2C and LILRB1 of dNK showed a similar trend with peripheral blood between HC and RSA\u2010A groups. The NKG2C<jats:sup>+<\/jats:sup> and LILRB1<jats:sup>+<\/jats:sup> NK cells were an independent risk factor for predicting pregnancy loss in RSA patients, with an area under the ROC curves (AUC) of .77 and .71 respectively.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Women with recurrent spontaneous abortion have abnormal NKG2C<jats:sup>+<\/jats:sup> and LILRB1<jats:sup>+<\/jats:sup> pNK subsets, which could reflect immune abnormalities at the maternal\u2010fetal interface, and NKG2C<jats:sup>+<\/jats:sup> and LILRB1<jats:sup>+<\/jats:sup> pNK subsets could be a good indicator for the prediction of pregnancy loss.<\/jats:p><\/jats:sec>","DOI":"10.1111\/aji.13627","type":"journal-article","created":{"date-parts":[[2022,9,23]],"date-time":"2022-09-23T19:48:50Z","timestamp":1663962530000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["The predictive value of NKG2C+NK cells and LILRB1+NK 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