{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,5]],"date-time":"2026-08-05T13:41:36Z","timestamp":1785937296995,"version":"3.56.0"},"reference-count":33,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2022,10,26]],"date-time":"2022-10-26T00:00:00Z","timestamp":1666742400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["American J Rep Immunol"],"published-print":{"date-parts":[[2023,6]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Problem<\/jats:title><jats:p>Endometriosis patients undergoing in vitro fertilization\u2010embryo transfer (IVF\u2010ET) treatment suffer from poor oocyte quality, a reduced likelihood of the fertilization rate, and low embryo quality. The dysregulation of immune cells and cytokine profiles in the follicular fluid (FF) may play an important role in the competence of the oocyte and the development of the embryo, but the mechanism remains largely unknown.<\/jats:p><\/jats:sec><jats:sec><jats:title>Method of Study<\/jats:title><jats:p>A total of 40 proved advanced staged endometriosis patients were enrolled in this study. The pregnancy results were followed until all the embryos collected by the first oocyte retrieval cycle were used up. The immune cells subtypes in FF and serum collected on the day of oocyte retrieval were detected by flow cytometry and 27 cytokines were determined using the Bio\u2010Plex Pro Human Cytokine 27\u2010Plex Immunoassay. The specific effect of cytokine on the gene expression of human granulosa cells was determined by RT\u2010qPCR.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>The fertilization rate and the cumulative live birth rate were significantly lower in the endometriosis group. The ratio of CD4<jats:sup>+<\/jats:sup>\/CD8<jats:sup>+<\/jats:sup>T cells in FF was significantly lower, while the level of IP\u201010, RANTES and G\u2010CSF were statistically higher in the endometriosis group. The level of IP\u201010 correlated with the IVF outcome. Moreover, treated by IP\u201010, the mRNA level of FSHR and CYP19A1 the human granulosa cells were downregulated in vitro.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>These results suggest that alterations of the lymphocyte subsets and cytokines in women with advanced endometriosis may have an impact on the oocyte development and resulting in poorer IVF outcomes.<\/jats:p><\/jats:sec>","DOI":"10.1111\/aji.13633","type":"journal-article","created":{"date-parts":[[2022,10,17]],"date-time":"2022-10-17T13:58:06Z","timestamp":1666015086000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":19,"title":["The cytokine profiles in follicular fluid and reproductive outcomes in women with endometriosis"],"prefix":"10.1111","volume":"89","author":[{"given":"Mu\u2010Tian","family":"Han","sequence":"first","affiliation":[{"name":"Center of Human Reproduction and Genetics The Affiliated Suzhou Hospital of Nanjing 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