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These events are regulated by embryo\u2010endometrium derived molecular factors which include hormones, growth factors, cytokines, immune\u2010modulators, cell adhesion molecules and proteases. Due to poor viability of blastocysts, they fail to hatch and implant, leading to a low \u2018Live Birth Rates\u2019, majorly contributing to infertility. Here, embryo\u2010derived biomarkers analysis plays a key role to assess potential biological viability of blastocysts which are capable of implantation and prospective pregnancy. Thus far, embryo\u2010derived biomarkers examined are mostly immune\u2010modulators which are thought to be associated with blastocyst development\u2010implantation and progression of pregnancy, leading to live births. There is an urgent need to develop a quantitative and a reliable non\u2010invasive approach aiding embryo selection for elective single embryo transfer and to minimize recurrent pregnancy loss and multiple pregnancies. In this article, we provide a comprehensive review on our current knowledge and understanding of potential embryo\u2010derived molecular regulators, that is, biomarkers, of development of human blastocysts, their hatching and implantation. We discuss their potential implications in the assessment of blastocyst implantation potential and pregnancy outcome in terms of live births in humans.<\/jats:p>","DOI":"10.1111\/aji.13635","type":"journal-article","created":{"date-parts":[[2022,10,18]],"date-time":"2022-10-18T06:30:00Z","timestamp":1666074600000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["Molecular regulators of human blastocyst development and hatching: Their significance in implantation and pregnancy outcome"],"prefix":"10.1111","volume":"89","author":[{"given":"Venkatappa","family":"Vani","sequence":"first","affiliation":[{"name":"Indian Institute of Science Department of Molecular Reproduction Development and Genetics Sir C.V. 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