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However, whether other mechanisms limit embryo growth in decidua containing <jats:italic>PBX1<\/jats:italic>\u2010deficient natural killer (NK) cells is unknown.<\/jats:p><\/jats:sec><jats:sec><jats:title>Method of study<\/jats:title><jats:p><jats:italic>Pbx1<jats:sup>f\/f<\/jats:sup>; Ncr1<jats:sup>Cre<\/jats:sup><\/jats:italic> mice were employed to explore the underlying mechanisms by which <jats:italic>PBX1<\/jats:italic><jats:sup>\u2212<\/jats:sup> NK cells affect embryonic development. To simulate the clinical testing of pregnant women, Doppler ultrasound imaging was used to detect embryo implantation and development. Differentially expressed genes (DEGs) in <jats:italic>PBX1<\/jats:italic><jats:sup>\u2212<\/jats:sup> NK cells that may affect normal pregnancy were screened using RNA\u2010sequencing and real\u2010time PCR. Immune cell changes caused by DEGs were detected by flow cytometry. Finally, the mechanism of FGR was explored by injecting the protein LCN2, corresponding to the selected DEG, into mice.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>We verified the embryonic dysplasia in pregnant <jats:italic>Pbx1<jats:sup>f\/f<\/jats:sup>; Ncr1<jats:sup>Cre<\/jats:sup><\/jats:italic> mice by Doppler ultrasound imaging and found that LCN2 was upregulated in dNK cells. We also observed higher infiltration of neutrophils and macrophages in the decidua of <jats:italic>Pbx1<jats:sup>f\/f<\/jats:sup>; Ncr1<jats:sup>Cre<\/jats:sup><\/jats:italic> mice. Finally, we found an increase in the number and activation of neutrophils at the maternal\u2010fetal interface after injecting LCN2 into pregnant mice and observed that these mice showed signs of FGR.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Excessive LCN2 secreted by <jats:italic>PBX1<\/jats:italic><jats:sup>\u2212<\/jats:sup> dNK cells at the maternal\u2010fetal interface recruit neutrophils and causes an inflammatory response, which is related to FGR.<\/jats:p><\/jats:sec>","DOI":"10.1111\/aji.13676","type":"journal-article","created":{"date-parts":[[2023,1,9]],"date-time":"2023-01-09T15:10:34Z","timestamp":1673277034000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["Overexpression of lipocalin 2 in <i>PBX1<\/i>\u2010deficient decidual NK cells promotes inflammation at the maternal\u2010fetal 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