{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,12]],"date-time":"2026-10-12T02:16:59Z","timestamp":1791771419411,"version":"4.3.4"},"reference-count":30,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2023,5,26]],"date-time":"2023-05-26T00:00:00Z","timestamp":1685059200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81901480"],"award-info":[{"award-number":["81901480"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["American J Rep Immunol"],"published-print":{"date-parts":[[2023,7]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Problem<\/jats:title><jats:p>Interferon\u2010\u03bb (IFN\u2010\u03bb) is a novel non\u2010redundant regulator that participates in the fetal\u2013maternal immune interaction, including immune regulation, uterine receptivity, cell migration and adhesion, and endometrium apoptosis. However, the exact transcriptional foundation for endometrial signaling of IFN\u2010\u03bb is not completely understood, and studies regarding IFN\u2010\u03bb to implantation failure in vivo are limited.<\/jats:p><\/jats:sec><jats:sec><jats:title>Method of study<\/jats:title><jats:p>The gene expression profile of human endometrial Ishikawa cell line treated with IFN\u2010\u03bb or IFN\u2010\u03b1 (100\u00a0ng\/mL) for 6\u00a0h was analyzed using RNA\u2010sequencing. Real\u2010time qPCR, western blotting, and enzyme\u2010linked immunosorbent assay (ELISA) tests were used to validate these sequencing data. An in vivo IFN\u2010\u03bb knock\u2010down mouse pregnancy model was performed, and the phenotype analysis and the intrauterine biomarkers detection were applied with the uterus samples.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>High levels of messenger RNA (mRNA) were detected for genes previously associated with endometrial receptivity, including <jats:italic>LIF<\/jats:italic>, <jats:italic>AXL<\/jats:italic>, <jats:italic>CRYAB<\/jats:italic>, <jats:italic>EPHB2<\/jats:italic>, <jats:italic>CCL5<\/jats:italic>, and <jats:italic>DDX58<\/jats:italic>, following IFN\u2010\u03bb treatment. Moreover, the data indicated IFN\u2010\u03bb reduced pro\u2010inflammatory gene activity compared with IFN\u2010\u03b1, including members of the ISG, TNF, SP100 and interleukin genes. The in vivo mouse pregnancy model showed that inhibition of intrauterine IFN\u2010\u03bb results in aberrant epithelial phenotype and significantly decreases the embryo implantation rates and derails normal uterine receptivity.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>These findings demonstrate the antagonistic and agonistic roles of IFNs in the endometrial cell, suggesting a selective role of IFN\u2010\u03bb in endometrial receptivity and immunological tolerance regulation. Moreover, the findings provide valuable insight into potential biomarkers related to endometrial receptivity and facilitate an understanding of the molecular changes observed during infertility treatment and contraception usage.<\/jats:p><\/jats:sec>","DOI":"10.1111\/aji.13718","type":"journal-article","created":{"date-parts":[[2023,5,27]],"date-time":"2023-05-27T05:34:45Z","timestamp":1685165685000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["The transcriptional foundations of interferon\u2010\u03bb\u2010mediated endometrial cell to uterine receptivity"],"prefix":"10.1111","volume":"90","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-0240-9056","authenticated-orcid":false,"given":"Kezhen","family":"Yao","sequence":"first","affiliation":[{"name":"Department of Reproductive Endocrinology Women's Hospital Zhejiang University School of Medicine  Hangzhou 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