{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,6]],"date-time":"2026-06-06T01:58:09Z","timestamp":1780711089505,"version":"3.54.1"},"reference-count":39,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2023,8,7]],"date-time":"2023-08-07T00:00:00Z","timestamp":1691366400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["American J Rep Immunol"],"published-print":{"date-parts":[[2023,9]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Preeclampsia (PE) is a hypertensive disorder of pregnancy that causes significant maternal and perinatal morbidity and mortality. Circular RNA (circRNA) hsa_circ_0015382 is associated with the pathogenesis of PE, but its underlying regulatory mechanism remains to be explored.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Relative RNA levels of hsa_circ_0015382, microRNA\u2010616\u20103p and thrombospondin\u20102 (THBS2) were detected by quantitative reverse transcription\u2010polymerase chain reaction. In vitro regulatory effects of hsa_circ_0015382 on the proliferation, migration, invasion and angiogenesis of trophoblasts were evaluated by CCK\u20108, flow cytometry for cell cycle, EdU, transwell, wound healing and HUVEC tube formation assays, respectively. Targeting interaction was verified by dual\u2010luciferase reporter and RNA immunoprecipitation assays.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Hsa_circ_0015382 was highly expressed in placental tissues from PE patients. Upregulation of hsa_circ_0015382 repressed trophoblast proliferation, migration, invasion and lowered trophoblast\u2010induced HUVEC tube formation. Hsa_circ_0015382 was validated as a miR\u2010616\u20103p sponge and miR\u2010616\u20103p targeted THBS2. Hsa_circ_0015382 could mediate trophoblast proliferation, migration, invasion and regulate trophoblast\u2010induced HUVEC tube formation by sponging miR\u2010616\u20103p and regulating THBS2 expression.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Hsa_circ_0015382 is associated with the pathogenesis of PPE by regulating the miR\u2010616\u20103p\/THBS2 axis.<\/jats:p><\/jats:sec><jats:sec><jats:title>Highlights<\/jats:title><jats:p><jats:list>\n<jats:list-item><jats:p>Hsa_circ_0015382 is overexpressed in preeclampsia patients.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>Hsa_circ_0015382 inhibits trophoblast proliferation, migration, invasion and decreases trophoblast\u2010induced HUVEC tube formation.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>Hsa_circ_0015382 interacts with miR\u2010616\u20103p to regulate THBS2 expression.<\/jats:p><\/jats:list-item>\n<\/jats:list><\/jats:p><\/jats:sec>","DOI":"10.1111\/aji.13760","type":"journal-article","created":{"date-parts":[[2023,8,7]],"date-time":"2023-08-07T07:01:27Z","timestamp":1691391687000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Hsa_circ_0015382 is involved in the pathogenesis of preeclampsia by mediating THBS2 expression"],"prefix":"10.1111","volume":"90","author":[{"given":"Yang","family":"Wang","sequence":"first","affiliation":[{"name":"Department of Obstetrics Sichuan Provincial Maternity and Child Health Care Hospital The Affiliated Women's and Children's Hospital of Chengdu Medical College Chengdu Sichuan Province China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lingfang","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Obstetrics Sichuan Provincial Maternity and Child Health Care Hospital The Affiliated Women's and Children's Hospital of Chengdu Medical College Chengdu Sichuan Province China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiayao","family":"Wang","sequence":"additional","affiliation":[{"name":"School of Clinical Medicine Chengdu Medical College Chengdu Sichuan Province China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0000-4411-2723","authenticated-orcid":false,"given":"Yan","family":"Gao","sequence":"additional","affiliation":[{"name":"Department of Obstetrics Sichuan Provincial Maternity and Child Health Care Hospital The Affiliated Women's and Children's Hospital of Chengdu Medical College Chengdu Sichuan Province China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2023,8,7]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1136\/bmj.l2381"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI91300"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00018-019-03104-6"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-981-13-1426-1_12"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tjog.2019.09.007"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.12659\/MSM.912801"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1080\/15476286.2019.1644591"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1042\/BSR20191965"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.5603\/EP.a2017.0060"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1071\/RD12384"},{"issue":"3","key":"e_1_2_7_12_1","article-title":"Expression of PAPPA2 in human fetomaternal interface and involvement in trophoblast invasion and migration","volume":"15","author":"Wang HY","year":"2016","journal-title":"Genet Mol Res"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41419-020-2679-6"},{"issue":"5","key":"e_1_2_7_14_1","first-page":"705","article-title":"Profiles of circular RNAs in human placenta and their potential roles related to preeclampsia","volume":"98","author":"Bai Y","year":"2018","journal-title":"Biol Reprod"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.placenta.2021.03.005"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcp.29286"},{"key":"e_1_2_7_17_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-981-13-1426-1_6"},{"key":"e_1_2_7_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrc.2018.05.109"},{"key":"e_1_2_7_19_1","doi-asserted-by":"publisher","DOI":"10.18632\/aging.202569"},{"key":"e_1_2_7_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.yexmp.2020.104492"},{"key":"e_1_2_7_21_1","doi-asserted-by":"publisher","DOI":"10.3892\/etm.2021.10093"},{"key":"e_1_2_7_22_1","doi-asserted-by":"publisher","DOI":"10.1111\/cpr.12490"},{"key":"e_1_2_7_23_1","doi-asserted-by":"publisher","DOI":"10.1002\/advs.202002483"},{"key":"e_1_2_7_24_1","doi-asserted-by":"publisher","DOI":"10.18632\/aging.103979"},{"key":"e_1_2_7_25_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41419-017-0077-5"},{"key":"e_1_2_7_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biopha.2018.10.009"},{"key":"e_1_2_7_27_1","doi-asserted-by":"publisher","DOI":"10.1006\/meth.2001.1262"},{"key":"e_1_2_7_28_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41581-019-0119-6"},{"key":"e_1_2_7_29_1","doi-asserted-by":"publisher","DOI":"10.1002\/mrd.23262"},{"issue":"24","key":"e_1_2_7_30_1","first-page":"5630","article-title":"MiR\u2010616 promotes proliferation and inhibits apoptosis in glioma cells by suppressing expression of SOX7 via the Wnt signaling pathway","volume":"21","author":"Bai QL","year":"2017","journal-title":"Eur Rev Med Pharmacol Sci"},{"key":"e_1_2_7_31_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.lfs.2019.116549"},{"issue":"3","key":"e_1_2_7_32_1","first-page":"2348","article-title":"miR\u2010616 promotes breast cancer migration and invasion by targeting TIMP2 and regulating MMP signaling","volume":"18","author":"Yuan C","year":"2019","journal-title":"Oncol Lett"},{"key":"e_1_2_7_33_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12094-020-02371-1"},{"key":"e_1_2_7_34_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12943-020-1135-7"},{"key":"e_1_2_7_35_1","doi-asserted-by":"publisher","DOI":"10.1101\/cshperspect.a009712"},{"key":"e_1_2_7_36_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12079-009-0060-8"},{"issue":"19","key":"e_1_2_7_37_1","first-page":"6230","article-title":"Knockdown of thrombospondin 2 inhibits metastasis through modulation of PI3K signaling pathway in uveal melanoma cell line M23","volume":"22","author":"Liu QH","year":"2018","journal-title":"Eur Rev Med Pharmacol Sci"},{"key":"e_1_2_7_38_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.placenta.2020.10.023"},{"key":"e_1_2_7_39_1","doi-asserted-by":"publisher","DOI":"10.1515\/med-2022-0427"},{"key":"e_1_2_7_40_1","doi-asserted-by":"publisher","DOI":"10.1002\/ddr.21931"}],"container-title":["American Journal of Reproductive Immunology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/aji.13760","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,8,5]],"date-time":"2024-08-05T10:01:13Z","timestamp":1722852073000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/aji.13760"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,8,7]]},"references-count":39,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2023,9]]}},"alternative-id":["10.1111\/aji.13760"],"URL":"https:\/\/doi.org\/10.1111\/aji.13760","archive":["Portico"],"relation":{},"ISSN":["1046-7408","1600-0897"],"issn-type":[{"value":"1046-7408","type":"print"},{"value":"1600-0897","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,8,7]]},"assertion":[{"value":"2022-12-22","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2023-07-07","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2023-08-07","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e13760"}}