{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,14]],"date-time":"2026-10-14T10:35:19Z","timestamp":1791974119529,"version":"4.4.0"},"reference-count":25,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2023,8,8]],"date-time":"2023-08-08T00:00:00Z","timestamp":1691452800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["American J Rep Immunol"],"published-print":{"date-parts":[[2023,9]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Problem<\/jats:title><jats:p>Pro\u2010inflammatory phenomena drive preterm delivery (PTD). Hydrogen sulfide is a gasotransmitter with anti\u2010inflammatory properties produced through the activity of the enzyme cystathionine\u2010\u03b3\u2010lyase (CSE), and its impact was studied in models of normal delivery and PTD in mice.<\/jats:p><\/jats:sec><jats:sec><jats:title>Method of study<\/jats:title><jats:p>Female <jats:italic>CSE<\/jats:italic><jats:sup>+\/+<\/jats:sup> and <jats:italic>CSE<\/jats:italic><jats:sup>\u2212\/\u2212<\/jats:sup> mice were mated with male <jats:italic>CSE<\/jats:italic><jats:sup>+\/+<\/jats:sup> mice; mating was done with drinking water unsupplemented and supplemented with cysteine. The pregnancy rate was monitored. PTD was induced by the intraperitoneal injection of bacterial lipopolysaccharide (LPS) on day 14.5 of pregnancy. Mice were sacrificed for tissue collection and splenocyte isolation after 6 and 12\u00a0h. Isolated splenocytes were stimulated for the production of tumor necrosis factor\u2010alpha (TNF\u03b1), interleukin (IL)\u201010 and interferon\u2010gamma (IFN\u03b3); TNF\u03b1 and vascular endothelial growth factor (VEGF) were measured in the fetuses and the placenta.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>The successful pregnancy rate was lower in <jats:italic>CSE<\/jats:italic><jats:sup>\u2212\/\u2212<\/jats:sup> mice and it was restored with cysteine supplementation. <jats:italic>CSE<\/jats:italic> deficiency was associated with higher tissue concentrations of TNF\u03b1 in the fetuses, attenuated IL\u201010 responses and higher IFN\u03b3 production from splenocytes. <jats:italic>CSE<\/jats:italic> deficiency was not associated with PTD. Following PTD induction, <jats:italic>CSE<\/jats:italic><jats:sup>\u2212\/\u2212<\/jats:sup> mice did not show attenuated IL\u201010 responses but the production of TNF\u03b1 and IFN\u03b3 was lowered over\u2010time; placental VEGF was also increased over\u2010time.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p><jats:italic>CSE<\/jats:italic> deficiency has an unfavorable impact on pregnancy. H<jats:sub>2<\/jats:sub>S deficiency through CSE does not drive PTD but mediates pro\u2010inflammatory phenomena in fetuses.<\/jats:p><\/jats:sec>","DOI":"10.1111\/aji.13764","type":"journal-article","created":{"date-parts":[[2023,8,9]],"date-time":"2023-08-09T04:41:56Z","timestamp":1691556116000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Deficiency of hydrogen sulfide production and pregnancy rate in an experimental model: Association with preterm delivery"],"prefix":"10.1111","volume":"90","author":[{"given":"Dimitrios","family":"Panagiotopoulos","sequence":"first","affiliation":[{"name":"3rd Department of Obstetrics and Gynecology National and Kapodistrian University of Athens Medical School Athens Greece"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Theano","family":"Andriopoulou","sequence":"additional","affiliation":[{"name":"4th Department of Internal Medicine National and 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