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Developing novel in vitro models that mimic in vivo uterine physiology is essential to study microbial pathogenesis. We utilized the feto\u2010maternal interface organ\u2010on\u2010chip (FMi\u2010OOC) device and determined the propagation of <jats:italic>Ureaplasma parvum<\/jats:italic>, and its impact on cell signaling and inflammation.<\/jats:p><\/jats:sec><jats:sec><jats:title>Method of study<\/jats:title><jats:p>FMi\u2010OOC is a microphysiologic device mimicking fetal membrane\/decidua interconnected through microchannels. The impact of resident decidual CD45<jats:sup>+<\/jats:sup> leukocytes was also determined by incorporating them into the decidual chamber in different combinations with <jats:italic>U. parvum<\/jats:italic>. We tested the propagation of live <jats:italic>U. parvum<\/jats:italic> from the decidual to the amniochorion membranes (immunocytochemistry and quantitative PCR), determined its impact on cytotoxicity (LDH assay), cell signaling (JESS<jats:sup>TM<\/jats:sup> Western Blot), cellular transition (immunostaining for vimentin and cytokeratin), and inflammation (cytokine bead array).<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p><jats:italic>U. parvum<\/jats:italic> transversed the chorion and reached the amnion epithelium after 72\u00a0hours but did not induce cell signaling kinases (p38MAPK and JNK) activation, or cellular transition (epithelial\u2010mesenchymal), regardless of the presence of immune cells. The inflammatory response was limited to the choriodecidual interface and did not promote inflammation in the amnion layer.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>Our data suggest that <jats:italic>U. parvum<\/jats:italic> is poorly immunogenic and does not produce massive inflammatory changes at the feto\u2010maternal interface. We speculate that the presence of <jats:italic>U. parvum<\/jats:italic> may still compromise the feto\u2010maternal interface making it susceptible to other pathogenic infection.<\/jats:p><\/jats:sec>","DOI":"10.1111\/aji.13770","type":"journal-article","created":{"date-parts":[[2023,9,4]],"date-time":"2023-09-04T04:05:54Z","timestamp":1693800354000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":11,"title":["Modeling an ascending infection by <i>Ureaplasma parvum<\/i> and its cell signaling and inflammatory response at the feto\u2010maternal interface"],"prefix":"10.1111","volume":"90","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-3868-2935","authenticated-orcid":false,"given":"Giovana Fernanda Cosi","family":"Bento","sequence":"first","affiliation":[{"name":"Department of Pathology Botucatu Medical School S\u00e3o Paulo State University  S\u00e3o Paulo Brazil"},{"name":"Division of Basic Science and Translational Research Department of Obstetrics &amp; Gynecology The University of Texas Medical Branch at Galveston  Texas USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lauren S.","family":"Richardson","sequence":"additional","affiliation":[{"name":"Division of Basic Science and Translational Research Department of Obstetrics &amp; Gynecology The University of Texas Medical Branch at Galveston  Texas USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M\u00e1rcia Guimar\u00e3es","family":"da Silva","sequence":"additional","affiliation":[{"name":"Department of Pathology Botucatu Medical School S\u00e3o Paulo State University  S\u00e3o Paulo Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ourlad Alzeus G.","family":"Tantengco","sequence":"additional","affiliation":[{"name":"Department of Physiology College of Medicine University of the Philippines Manila  Manila Philippines"},{"name":"Department of Biology College of Science De La Salle University  Manila Philippines"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9213-6105","authenticated-orcid":false,"given":"Ramkumar","family":"Menon","sequence":"additional","affiliation":[{"name":"Division of Basic Science and Translational Research Department of Obstetrics &amp; Gynecology The University of Texas Medical Branch at Galveston  Texas USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2023,9,3]]},"reference":[{"key":"e_1_2_8_2_1","unstructured":"Organization W\u2010WH. 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