{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,5]],"date-time":"2026-09-05T18:07:24Z","timestamp":1788631644650,"version":"build-2803163510"},"reference-count":33,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2023,10,20]],"date-time":"2023-10-20T00:00:00Z","timestamp":1697760000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["American J Rep Immunol"],"published-print":{"date-parts":[[2023,11]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Problem<\/jats:title><jats:p>The association of viruses with infertility remains incompletely evaluated.<\/jats:p><\/jats:sec><jats:sec><jats:title>Method of study<\/jats:title><jats:p>Vaginal secretions from 46 women seeking treatment in the Center for Reproductive Medicine and Infertility at Weill Cornell Medicine were tested for viruses by metagenomic analysis by lab personnel blinded to all clinical data.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Torquetenovirus (TTV) was identified in 16 women, alphapapillomavirus in seven women and most were positive for bacteriophages. Twelve of the subjects were fertile and sought to freeze their oocytes for future implantation. These women were all negative for TTV. In contrast, 16 of the 34 women (47.1%) being treated for infertility were TTV\u2010positive (<jats:italic>p<\/jats:italic>\u00a0=\u00a0.0035). Evaluating the women by cause of infertility, five of nine women (55.6%) whose male partner had inadequate sperm parameters and six of 14 women (42.9%) with defective ovulation were TTV positive (<jats:italic>p<\/jats:italic>\u00a0=\u00a0.0062 and <jats:italic>p<\/jats:italic>\u00a0=\u00a0.0171, respectively, vs. the fertile women). Alphapapillomavirus was identified in one (8.3%) fertile woman, five (35.7%) women with ovulation deficiency, and one (11.1%) woman with male factor infertility. These differences were not statistically significant. There were no differences in bacteriophage families or the presence of <jats:italic>Lactobacillus<\/jats:italic> phages between fertile or infertile women or between different causes of infertility. There was a negative association between TTV detection and <jats:italic>Lactobacillus crispatus<\/jats:italic> dominance in the vaginal microbiota (<jats:italic>p<\/jats:italic>\u00a0=\u00a0.0184), but no association between TTV detection and the presence of alphapapillomavirus or <jats:italic>Candida<\/jats:italic> species.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Detection of TTV in the vagina might be a biomarker for specific causes of infertility.<\/jats:p><\/jats:sec>","DOI":"10.1111\/aji.13788","type":"journal-article","created":{"date-parts":[[2023,10,20]],"date-time":"2023-10-20T10:39:50Z","timestamp":1697798390000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Association between torquetenovirus in vaginal secretions and infertility: An exploratory metagenomic analysis"],"prefix":"10.1111","volume":"90","author":[{"given":"A. Charlys","family":"Da Costa","sequence":"first","affiliation":[{"name":"Laboratory of Investigative Medicine in Virology (LIM 52) Department of Infectious Diseases Institute of Tropical Medicine University of Sao Paulo  Sao Paulo Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pietro","family":"Bortoletto","sequence":"additional","affiliation":[{"name":"Boston IVF  Waltham Massachusetts USA"},{"name":"Harvard Medical School  Boston Massachusetts USA"},{"name":"Department of Obstetrics and Gynecology Beth Israel Deaconess Medical Center  Boston Massachusetts USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Steven D.","family":"Spandorfer","sequence":"additional","affiliation":[{"name":"Center for Reproductive Medicine and Infertility Weill Cornell Medicine  New York USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tania Regina","family":"Tozetto\u2010Mendoza","sequence":"additional","affiliation":[{"name":"Laboratory of 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