{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,10]],"date-time":"2026-09-10T22:51:47Z","timestamp":1789080707102,"version":"build-2803163510"},"reference-count":57,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2024,2,4]],"date-time":"2024-02-04T00:00:00Z","timestamp":1707004800000},"content-version":"vor","delay-in-days":3,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["American J Rep Immunol"],"published-print":{"date-parts":[[2024,2]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Problem<\/jats:title><jats:p>This study analyzed the contribution of <jats:italic>DRB1<\/jats:italic>, <jats:italic>DQB1<\/jats:italic>, and <jats:italic>DPB1<\/jats:italic> HLA class II alleles and resulting 3\u2010locus haplotypes to the overall risk of idiopathic recurrent pregnancy loss (RPL).<\/jats:p><\/jats:sec><jats:sec><jats:title>Method of study<\/jats:title><jats:p>The study participants included 188 Tunisian participants comprising 84 women with established diagnoses of RPL, and 104 matched multiparous females as controls. Genotyping of HLA alleles was done by PCR\u2010SSP.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>The allelic frequencies of <jats:italic>DPB1, DRB1<\/jats:italic>, and <jats:italic>DQB1<\/jats:italic> were consistent with Hardy\u2010Weinberg equilibrium among control subjects. Among class II alleles, <jats:italic>DRB1*04:01:01<\/jats:italic> (<jats:italic>p<\/jats:italic>\u00a0=\u00a0.03), <jats:italic>DRB1*08:01:01<\/jats:italic> (<jats:italic>p<\/jats:italic>\u00a0=\u00a0.04)<jats:italic>, and DPB1*01:01:01<\/jats:italic> (<jats:italic>p<\/jats:italic>\u00a0=\u00a0.04) were positively associated, whereas <jats:italic>DPB1*04:01:01<\/jats:italic> (<jats:italic>p<\/jats:italic>\u00a0=\u00a0.012) and <jats:italic>DPB1*14:01:01<\/jats:italic> (<jats:italic>p<\/jats:italic>\u00a0=\u00a0.006) were negatively associated with risk of RPL. The association of <jats:italic>DRB1 and DPB1<\/jats:italic> alleles with RPL was lost after correction for multiple comparisons. The prevalence of the seven determined <jats:italic>DQB1<\/jats:italic> alleles was not significantly different among case and control groups. Higher prevalence of <jats:italic>DRB1*07:01:01\u223c DQB1*02:01:01\u223cDPB1*04:01:01<\/jats:italic> (0.0417\u00a0vs. 0.000; <jats:italic>p<\/jats:italic>\u00a0=\u00a0.049) coupled with lower prevalence of <jats:italic>DRB1*13:01:01\u223cDQB1*06:01:01\u223cDPB1*02:01:01<\/jats:italic> (0.0167\u00a0vs. 0.1000; <jats:italic>p<\/jats:italic>\u00a0=\u00a0.014) haplotypes was noted in women with RPL, thus could be considered as an RPL at\u2010risk and RPL\u2010protective haplotype, respectively.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>The study establishes weak\/no contribution of class II alleles and corresponding 3\u2010locus haplotypes to the altered risk of RPL among Tunisian women and does not confirm previous findings on other Arab\u2010speaking populations of an HLA association with RPL.<\/jats:p><\/jats:sec>","DOI":"10.1111\/aji.13817","type":"journal-article","created":{"date-parts":[[2024,2,5]],"date-time":"2024-02-05T04:25:33Z","timestamp":1707107133000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Maternal HLA class II alleles and haplotypes associated with altered risk of recurrent pregnancy loss: A case\u2010control study"],"prefix":"10.1111","volume":"91","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-2868-4497","authenticated-orcid":false,"given":"Gulzhanat","family":"Aimagambetova","sequence":"first","affiliation":[{"name":"Department of Surgery School of Medicine Nazarbayev University  Astana Kazakhstan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aizhan","family":"Kapasheva","sequence":"additional","affiliation":[{"name":"Department of Biomedical Sciences School of Medicine Nazarbayev University  Astana Kazakhstan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5883-7415","authenticated-orcid":false,"given":"Wael","family":"Bahia","sequence":"additional","affiliation":[{"name":"Faculty of Pharmacy University of Monastir  Monastir Tunisia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kuralay","family":"Atageldiyeva","sequence":"additional","affiliation":[{"name":"Department of Medicine School of Medicine Nazarbayev University  Astana Kazakhstan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1633-9757","authenticated-orcid":false,"given":"Wassim Y.","family":"Almawi","sequence":"additional","affiliation":[{"name":"Department of Biomedical Sciences School of Medicine Nazarbayev University  Astana Kazakhstan"},{"name":"Faculte\u2019 des Sciences de Tunis Universite\u2019 de Tunis El Manar  Tunis Tunisia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2024,2,4]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.3390\/jcm12124074"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.rbmo.2019.04.008"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1160\/TH05-07-0525"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1093\/hropen\/hoad002"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1111\/aji.13173"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.3390\/jcm12020658"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1002\/ijgo.14701"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ejogrb.2012.04.001"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12967-018-1453-3"},{"issue":"4","key":"e_1_2_8_11_1","first-page":"770","article-title":"HLA class II profile and distribution of HLA\u2010DRB1 and HLA\u2010DQB1 alleles and haplotypes among Lebanese and Bahraini Arabs","volume":"11","author":"Almawi WY","year":"2004","journal-title":"Clin Diagn Lab Immunol"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0192269"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1038\/jhg.2008.5"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1002\/iid3.416"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00018-022-04491-z"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.1128\/CVI.00099-10"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.1111\/tan.14007"},{"key":"e_1_2_8_18_1","doi-asserted-by":"publisher","DOI":"10.1111\/tan.14057"},{"key":"e_1_2_8_19_1","doi-asserted-by":"publisher","DOI":"10.1055\/a-0754-5586"},{"key":"e_1_2_8_20_1","doi-asserted-by":"publisher","DOI":"10.1111\/tan.12855"},{"key":"e_1_2_8_21_1","doi-asserted-by":"publisher","DOI":"10.1210\/jc.2005-1166"},{"key":"e_1_2_8_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jri.2016.07.006"},{"key":"e_1_2_8_23_1","doi-asserted-by":"publisher","DOI":"10.29252\/ijrm.15.9.569"},{"key":"e_1_2_8_24_1","doi-asserted-by":"publisher","DOI":"10.1038\/srep35005"},{"key":"e_1_2_8_25_1","doi-asserted-by":"publisher","DOI":"10.1111\/aji.12643"},{"key":"e_1_2_8_26_1","doi-asserted-by":"publisher","DOI":"10.1093\/humrep\/der024"},{"key":"e_1_2_8_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.autrev.2016.02.009"},{"key":"e_1_2_8_28_1","doi-asserted-by":"publisher","DOI":"10.1093\/humrep\/deh200"},{"key":"e_1_2_8_29_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jri.2018.06.024"},{"key":"e_1_2_8_30_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms21134756"},{"key":"e_1_2_8_31_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms24032557"},{"key":"e_1_2_8_32_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0897.1999.tb00096.x"},{"key":"e_1_2_8_33_1","doi-asserted-by":"publisher","DOI":"10.1128\/CVI.00215-08"},{"key":"e_1_2_8_34_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.humimm.2015.02.004"},{"key":"e_1_2_8_35_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1399-0039.2006.00769.x"},{"key":"e_1_2_8_36_1","first-page":"47","article-title":"Arlequin (version 3.0): an integrated software package for population genetics data analysis","volume":"1","author":"Excoffier L","year":"2007","journal-title":"Evol Bioinform Online"},{"key":"e_1_2_8_37_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0897.2008.00592.x"},{"key":"e_1_2_8_38_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2023.1069974"},{"key":"e_1_2_8_39_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12920-020-00730-z"},{"key":"e_1_2_8_40_1","doi-asserted-by":"publisher","DOI":"10.1093\/humrep\/deu043"},{"issue":"1","key":"e_1_2_8_41_1","first-page":"39","article-title":"Genetic factors of idiopathic recurrent miscarriage in Kazakh population","volume":"23","author":"Svyatova GS","year":"2022","journal-title":"J Reprod Infertil"},{"key":"e_1_2_8_42_1","doi-asserted-by":"publisher","DOI":"10.1111\/tan.12713"},{"key":"e_1_2_8_43_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1399-0039.2007.00932.x"},{"key":"e_1_2_8_44_1","doi-asserted-by":"publisher","DOI":"10.1093\/humrep\/deaa113"},{"key":"e_1_2_8_45_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.preghy.2019.01.004"},{"key":"e_1_2_8_46_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.humimm.2015.11.016"},{"key":"e_1_2_8_47_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.imlet.2017.02.014"},{"key":"e_1_2_8_48_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1744-313X.2012.01155.x"},{"key":"e_1_2_8_49_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jri.2021.103308"},{"key":"e_1_2_8_50_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.clim.2005.07.011"},{"key":"e_1_2_8_51_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tjog.2021.11.039"},{"key":"e_1_2_8_52_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2021.797360"},{"key":"e_1_2_8_53_1","doi-asserted-by":"publisher","DOI":"10.1155\/2022\/8005538"},{"key":"e_1_2_8_54_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0897.1999.tb00474.x"},{"key":"e_1_2_8_55_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1246-7820(05)80041-2"},{"key":"e_1_2_8_56_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jri.2021.103467"},{"key":"e_1_2_8_57_1","doi-asserted-by":"publisher","DOI":"10.1111\/aji.12234"},{"key":"e_1_2_8_58_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms241310958"}],"updated-by":[{"DOI":"10.1111\/aji.13925","type":"correction","label":"Correction","source":"publisher","updated":{"date-parts":[[2024,9,3]],"date-time":"2024-09-03T00:00:00Z","timestamp":1725321600000}}],"container-title":["American Journal of Reproductive Immunology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/aji.13817","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,3,20]],"date-time":"2024-03-20T05:27:16Z","timestamp":1710912436000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/aji.13817"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,2]]},"references-count":57,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2024,2]]}},"alternative-id":["10.1111\/aji.13817"],"URL":"https:\/\/doi.org\/10.1111\/aji.13817","archive":["Portico"],"relation":{"correction":[{"id-type":"doi","id":"10.1111\/aji.13925","asserted-by":"object"}]},"ISSN":["1046-7408","1600-0897"],"issn-type":[{"value":"1046-7408","type":"print"},{"value":"1600-0897","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,2]]},"assertion":[{"value":"2023-09-14","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-01-14","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-02-04","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e13817"}}