{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,21]],"date-time":"2026-02-21T06:47:07Z","timestamp":1771656427768,"version":"3.50.1"},"reference-count":39,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2024,2,20]],"date-time":"2024-02-20T00:00:00Z","timestamp":1708387200000},"content-version":"vor","delay-in-days":19,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001411","name":"Indian Council of Medical Research","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100001411","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["American J Rep Immunol"],"published-print":{"date-parts":[[2024,2]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Problem<\/jats:title><jats:p>Increased oxidative stress (OS) and inflammatory responses are major underlying factors behind <jats:italic>Chlamydia trachomatis<\/jats:italic>\u2010associated recurrent spontaneous abortion (RSA). miRNAs are known to regulate inflammation and OS and their dysregulation has been associated with compromised pregnancies. Therefore, aim of this study was to investigate the expression\/correlation of OS biomarkers, cytokines and miRNAs in <jats:italic>C. trachomatis<\/jats:italic>\u2010associated RSA.<\/jats:p><\/jats:sec><jats:sec><jats:title>Method of Study<\/jats:title><jats:p>Urine and non\u2010heparinized blood samples were collected from RSA patients with history of &gt;3 consecutive abortions (cases) and non\u2010pregnant women with history of &gt;2 successful deliveries (controls) attending Department of Obstetrics and Gynaecology, Safdarjung hospital, New Delhi. <jats:italic>C. trachomatis<\/jats:italic> detection was done in urine by PCR. miRNA expression was studied by microarray analysis and validated by real time\u2010PCR. Evaluation of cytokines and antioxidant genes expression were done by real\u2010time PCR. Level of OS biomarkers 8\u2010hydroxy guanosine (8\u2010OHdG) and 8\u2010isporostane (8\u2010IP) were measured by ELISA.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Fifty circulating miRNAs were differentially expressed in infected patients compared with controls. Of these, four were overexpressed and 46 downregulated. Thirteen differentially expressed circulating miRNAs were selected to validate microarray results. miRs\u20108069, \u20103663\u20103p showed maximum upregulation\/downregulation in infected versus control group. Expression of cytokines (IL\u20108, TNF\u2010\u03b1, IFN\u2010\u03b3), antioxidant genes SOD2 and OS biomarkers (8\u2010OHdG,8\u2010IP) were increased while SOD1 was decreased in infected patients. miR\u20108069 showed significant positive correlation with cytokines, SOD2, 8\u2010OHdG and 8\u2010IP. miR\u20103663\u20103p showed significant positive correlation with SOD1.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>Overall results indicate circulating miRNAs are involved in pathogenesis of <jats:italic>C. trachomatis<\/jats:italic>\u2010associated RSA and are potential modulators of cytokine signalling and OS in infected RSA.<\/jats:p><\/jats:sec>","DOI":"10.1111\/aji.13821","type":"journal-article","created":{"date-parts":[[2024,2,20]],"date-time":"2024-02-20T08:25:57Z","timestamp":1708417557000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["MicroRNA mediated regulation of 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