{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T11:17:29Z","timestamp":1791199049900,"version":"4.1.0"},"reference-count":49,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2024,3,11]],"date-time":"2024-03-11T00:00:00Z","timestamp":1710115200000},"content-version":"vor","delay-in-days":10,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82071617"],"award-info":[{"award-number":["82071617"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["American J Rep Immunol"],"published-print":{"date-parts":[[2024,3]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>Endometritis is an inflammatory reaction of the lining of uterus, leading to the occurrence of infertility. Platelet rich plasma (PRP) has been proven to exhibit extremely effective for the treatment of endometrium\u2010associated infertility, but the mechanism of its prevention for endometritis remains unclear.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Objective<\/jats:title>\n                    <jats:p>The present study aimed to investigate the protective effect of PRP against endometritis induced by lipopolysaccharide (LPS) and elucidate the mechanism underlying these effects.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>Mouse model of endometritis was established by intrauterine perfusion of LPS. PRP intrauterine infusion was administered at 24\u00a0h after LPS induction. After another 24\u00a0h, the uterine tissues were harvested to observe histopathological changes, production of proinflammatory cytokines, variation of the Toll\u2010like receptor 4\/nuclear factor \u03baB (TLR4\/NF\u2010\u03baB) signaling pathways, and validated the anti\u2010inflammatory effect of PRP. The myeloperoxidase (MPO) activity and concentration of nitric oxide (NO) were determined using assay kit. Proinflammatory chemokines (tumor necrosis factor\u2010\u03b1 (TNF\u2010\u03b1), interleukin\u20101\u03b2 (IL\u20101\u03b2), and interleukin\u20106 (IL\u20106)) were measured by ELISA and Real\u2010Time PCR. The activity of TLR4\/NF\u2010\u03baB pathway in uterine tissues was measured by Western blotting.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>Hematoxylin\u2010eosin staining (H&amp;E) appeared that PRP remarkably relieved the impairment of uterine tissues. Detection of MPO activity and concentration of NO revealed that PRP treatment distinctly mitigated infiltration of inflammatory cells in mice with endometritis induced by LPS. PRP treatment significantly affected the expression of TNF\u2010\u03b1, IL\u20101\u03b2, and IL\u20106. PRP was also found to suppress LPS\u2010induced activation of TLR4\/NF\u2010\u03baB pathway.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>PRP effectively alleviates LPS\u2010induced endometritis via restraining the signal pathway of TLR4\/NF\u2010\u03baB. These findings provide a solid foundation for PRP as a potential therapeutic agent for endometritis.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/aji.13833","type":"journal-article","created":{"date-parts":[[2024,3,11]],"date-time":"2024-03-11T23:59:41Z","timestamp":1710201581000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":16,"title":["Platelet rich plasma alleviates endometritis induced by lipopolysaccharide in mice via inhibiting TLR4\/NF\u2010\u03baB signaling pathway"],"prefix":"10.1111","volume":"91","author":[{"given":"Xiaoqiang","family":"Liu","sequence":"first","affiliation":[{"name":"Center for Reproductive Medicine Cheeloo College of Medicine Shandong University  Jinan Shandong China"},{"name":"Key Laboratory of Reproductive Endocrinology of Ministry of Education Shandong University  Jinan Shandong China"},{"name":"Reproductive Medicine Center Qingdao Women and Children's Hospital  Qingdao Shandong 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