{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,25]],"date-time":"2026-06-25T15:20:32Z","timestamp":1782400832577,"version":"3.54.5"},"reference-count":38,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2024,5,6]],"date-time":"2024-05-06T00:00:00Z","timestamp":1714953600000},"content-version":"vor","delay-in-days":5,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["American J Rep Immunol"],"published-print":{"date-parts":[[2024,5]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>The amniotic membrane (AM) has shown immense potential in repairing wounds due to its great regenerative qualities. Although the role of AM as a biological scaffold in repairing wounds has been studied well, the tissue regenerative potential of AM\u2010derived mesenchymal stem cells (MSCs) and conditioned media (CM) derived from it remains to be discovered as of now. Here, we examined the wound healing abilities of fresh and frozen thawed rabbit AM (rAM) along with the MSCs and their lyophilised CM in rabbits challenged with skin wounds.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>To elucidate the role of rAM\u2010MSCs and its CM in repairing the wound, we isolated it\u00a0from the freshly derived placenta and characterised their differentiation potential by performing an in vitro tri\u2010lineage differentiation assay besides other standard confirmations. We compared the wound repair capacities of rAM\u2010MSCs and lyophilised CM with the fresh and cryopreserved AM at different timelines by applying them to excision wounds created in rabbits.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>By monitoring wound contractions and tissue histology of wounded skin at different time points after the application, we observed that rAM\u2010MSCs and rAM\u2010MSC\u2010derived CM significantly promoted wound closure compared to the control group. We also observed that the wound closure capacity of rAM\u2010MSCs and rAM\u2010MSC\u2010derived CM is as efficient as fresh and cryopreserved rAM.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Our findings suggest that rAM\u2010MSCs and rAM\u2010MSC derived CM can be effectively used to treat skin wounds in animals and correctly delivered to the damaged tissue using AM as a bioscaffold, either fresh or frozen.<\/jats:p><\/jats:sec>","DOI":"10.1111\/aji.13853","type":"journal-article","created":{"date-parts":[[2024,5,6]],"date-time":"2024-05-06T07:29:27Z","timestamp":1714980567000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Exploring the therapeutic potential of allogeneic amniotic membrane for quality wound healing in rabbit model"],"prefix":"10.1111","volume":"91","author":[{"given":"Likhitha","family":"BN","sequence":"first","affiliation":[{"name":"Division of Physiology and Climatology ICAR\u2010Indian Veterinary Research Institute (IVRI)  Izatnagar U.P. 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