{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,25]],"date-time":"2026-01-25T00:24:45Z","timestamp":1769300685296,"version":"3.49.0"},"reference-count":40,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2024,5,26]],"date-time":"2024-05-26T00:00:00Z","timestamp":1716681600000},"content-version":"vor","delay-in-days":25,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["American J Rep Immunol"],"published-print":{"date-parts":[[2024,5]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Problem<\/jats:title><jats:p>Hypertensive disorders of pregnancy (HDP) are a common pregnancy disease. NANOG and Cyclin\u2010dependent kinase 1 (CDK1) are essential for regulating the function of cell proliferation and apoptosis. However, the mechanism of action in HDP is yet unclear.<\/jats:p><\/jats:sec><jats:sec><jats:title>Method<\/jats:title><jats:p>The microarray dataset GSE6573 was downloaded from the GEO database. Emt\u2010related gene set was downloaded from Epithelial\u2010Mesenchymal Transition gene database 2.0 were screened differentially expressed genes by bioinformatics analysis. Pathway Commons and Scansite 4.0 databases were used to predict the interaction between proteins. Placental tissue samples were collected from HDP patients and patients with uneventful pregnancies. RT\u2010qPCR, Western blot and immunohistochemistry were used to detect the expression of NANOG, CDK1, MMP\u20102, MMP\u20109, EMT markers and the JAK\/STAT3 pathway proteins. Transfection NANOG overexpression\/knockdown, and CDK1 knockdown into the human chorionic trophoblast cells (HTR\u20108\/Svneo). CCK\u20108, Transwell and Wound\u2010healing assay were used to evaluate cell proliferation, invasion and migration. CO\u2010IP and GST pull\u2010down assays were used to confirm the protein interaction.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>A total obtained seven EMT\u2010related differentially expressed genes, wherein NANOG, NODAL and LIN28A had protein interaction. In the HDP patients' tissue found that NANOG and CDK1 had lower expression. NANOG overexpression promoted HTR\u20108\/Svneo proliferation, migration and EMT, while NANOG knockdown had the opposite effect. Further a protein interaction between STAT3 and CDK1 with NANOG. NANOG overexpression downregulated the JAK\/STAT3 pathway to promote HTR\u20108\/Svneo proliferation, migration and EMT, which was reversed by CDK1 knockdown.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>NANOG downregulated the JAK\/STAT3 pathway to promote trophoblast cell proliferation, migration and EMT through protein interaction with CDK1.<\/jats:p><\/jats:sec>","DOI":"10.1111\/aji.13863","type":"journal-article","created":{"date-parts":[[2024,5,26]],"date-time":"2024-05-26T10:50:00Z","timestamp":1716720600000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["NANOG regulate the JAK\/STAT3 pathway to promote trophoblast cell migration and epithelial\u2010mesenchymal transition (EMT) in hypertensive disorders of pregnancy (HDP) through protein interaction with 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