{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,4]],"date-time":"2026-06-04T05:27:43Z","timestamp":1780550863739,"version":"3.54.1"},"reference-count":26,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2024,7,3]],"date-time":"2024-07-03T00:00:00Z","timestamp":1719964800000},"content-version":"vor","delay-in-days":2,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["American J Rep Immunol"],"published-print":{"date-parts":[[2024,7]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:sec><jats:title>Problem<\/jats:title><jats:p>The decidualization process conditions monocytes to the immunosuppressive and tolerogenic dendritic cell (DC)\u201010 profile, a DC subset with high IL\u201010 production. Since the implantation process implies an embryo\u2010endometrium\u2010immune crosstalk, here we focused on the ability of embryonic soluble factors to modify decidual DC conditioning accordingly with its quality.<\/jats:p><\/jats:sec><jats:sec><jats:title>Method of Study<\/jats:title><jats:p>Human endometrial stromal cell line (HESC) decidualized with medroxyprogesterone and dibutyryl\u2010cAMP (Dec) was stimulated with human embryo\u2010conditioned media (ECM), classified as normal (ND) or impaired developed (ID) for 48\u00a0h (<jats:italic>n<\/jats:italic> = 18\/group). Monocytes isolated from six healthy women were differentiated to DCs with rhGM\u2010CSF+rhIL\u20104 in the presence\/absence of conditioned media (CM) from decidualized cells stimulated with ECM or nontreated.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>We found that decidualized cells stimulated with ECM sustain a myeloid regulatory cell profile on monocyte\u2010derived culture with increased frequency of CD1a<jats:sup>\u2010<\/jats:sup>CD14<jats:sup>+<\/jats:sup> and CD83<jats:sup>+<\/jats:sup>CD86<jats:sup>low<\/jats:sup> cells. ND\u2010Dec sustained the higher expression of the DC\u201010 markers, HLA\u2010G and IL\u201010 whereas ID\u2010Dec diminished IL\u201010 production (ID\u2010Dec: 135 \u00b1 37.4\u00a0vs. Dec: 223.3 \u00b1 49.9 pg\/mL, <jats:italic>p\u00a0<\/jats:italic>&lt;\u00a00.05). The treatment with ECM\u2010Dec sustained a higher IL\u201010 production and prevented the increase of CD83\/CD86 after LPS challenge regardless of embryo quality. Notably, TNF\u2010\u03b1 production increased in ID\u2010Dec cultures (ID\u2010Dec: 475.1 \u00b1 134.7\u00a0vs. Dec: 347.5 \u00b1 98 pg\/mL, <jats:italic>p\u00a0<\/jats:italic>&lt;\u00a00.05).<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>Although remaining in a tolerogenic profile compatible with DC\u201010, DCs can differentially respond to decidual secreted factors based on embryo quality, changing their secretome. These results suggest that in the presence of arrested embryo, DCs could differentially shape the immunological microenvironment, contributing to arrested embryo clearance during the menstrual phase.<\/jats:p><\/jats:sec>","DOI":"10.1111\/aji.13891","type":"journal-article","created":{"date-parts":[[2024,7,3]],"date-time":"2024-07-03T11:46:59Z","timestamp":1720007219000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Embryo Quality Conditions the Secretory Profile of Tolerogenic Dendritic Cell DC\u201010 During the Peri\u2010Implantation Period"],"prefix":"10.1111","volume":"92","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-9516-8003","authenticated-orcid":false,"given":"Soledad","family":"Gori","sequence":"first","affiliation":[{"name":"Universidad de Buenos Aires (UBA), Consejo Nacional de Investigaciones Cient\u00edficas y T\u00e9cnicas (CONICET), Instituto de Qu\u00edmica Biol\u00f3gica de la Facultad de Ciencias Exactas y Naturales (IQUIBICEN\u2010CONICET), Laboratorio de Inmunofarmacolog\u00eda, Facultad de Ciencias Exactas y Naturales (FCEN\u2010UBA) Buenos Aires Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Laura","family":"Fern\u00e1ndez","sequence":"additional","affiliation":[{"name":"Universidad de Buenos Aires (UBA), Consejo Nacional de Investigaciones Cient\u00edficas y T\u00e9cnicas (CONICET), Instituto de Qu\u00edmica Biol\u00f3gica de la Facultad de Ciencias Exactas y Naturales (IQUIBICEN\u2010CONICET), Laboratorio de Inmunofarmacolog\u00eda, Facultad de Ciencias Exactas y Naturales (FCEN\u2010UBA) Buenos Aires Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elizabeth","family":"Soczewski","sequence":"additional","affiliation":[{"name":"Universidad de Buenos Aires (UBA), Consejo Nacional de Investigaciones Cient\u00edficas y T\u00e9cnicas (CONICET), Instituto de Qu\u00edmica Biol\u00f3gica de la Facultad de Ciencias Exactas y Naturales (IQUIBICEN\u2010CONICET), Laboratorio de Inmunofarmacolog\u00eda, Facultad de Ciencias Exactas y Naturales (FCEN\u2010UBA) Buenos Aires Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ana","family":"Schafir","sequence":"additional","affiliation":[{"name":"Universidad de Buenos Aires (UBA), Consejo Nacional de Investigaciones Cient\u00edficas y T\u00e9cnicas (CONICET), Instituto de Qu\u00edmica Biol\u00f3gica de la Facultad de Ciencias Exactas y Naturales (IQUIBICEN\u2010CONICET), Laboratorio de Inmunofarmacolog\u00eda, Facultad de Ciencias Exactas y Naturales (FCEN\u2010UBA) Buenos Aires Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lara","family":"Castagnola","sequence":"additional","affiliation":[{"name":"Universidad de Buenos Aires (UBA), Consejo Nacional de Investigaciones Cient\u00edficas y T\u00e9cnicas (CONICET), Instituto de Qu\u00edmica Biol\u00f3gica de la Facultad de Ciencias Exactas y Naturales (IQUIBICEN\u2010CONICET), Laboratorio de Inmunofarmacolog\u00eda, Facultad de Ciencias Exactas y Naturales (FCEN\u2010UBA) Buenos Aires Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Esteban","family":"Grasso","sequence":"additional","affiliation":[{"name":"Universidad de Buenos Aires (UBA), Consejo Nacional de Investigaciones Cient\u00edficas y T\u00e9cnicas (CONICET), Instituto de Qu\u00edmica Biol\u00f3gica de la Facultad de Ciencias Exactas y Naturales (IQUIBICEN\u2010CONICET), Laboratorio de Inmunofarmacolog\u00eda, Facultad de Ciencias Exactas y Naturales (FCEN\u2010UBA) Buenos Aires Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gustavo","family":"Mart\u00ednez","sequence":"additional","affiliation":[{"name":"Fertilis Medicina Reproductiva  San Isidro Buenos Aires Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8609-2617","authenticated-orcid":false,"given":"Claudia P\u00e9rez","family":"Leir\u00f3s","sequence":"additional","affiliation":[{"name":"Universidad de Buenos Aires (UBA), Consejo Nacional de Investigaciones Cient\u00edficas y T\u00e9cnicas (CONICET), Instituto de Qu\u00edmica Biol\u00f3gica de la Facultad de Ciencias Exactas y Naturales (IQUIBICEN\u2010CONICET), Laboratorio de Inmunofarmacolog\u00eda, Facultad de Ciencias Exactas y Naturales (FCEN\u2010UBA) Buenos Aires Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4222-817X","authenticated-orcid":false,"given":"Rosanna","family":"Ramhorst","sequence":"additional","affiliation":[{"name":"Universidad de Buenos Aires (UBA), Consejo Nacional de Investigaciones Cient\u00edficas y T\u00e9cnicas (CONICET), Instituto de Qu\u00edmica Biol\u00f3gica de la Facultad de Ciencias Exactas y Naturales (IQUIBICEN\u2010CONICET), Laboratorio de Inmunofarmacolog\u00eda, Facultad de Ciencias Exactas y Naturales (FCEN\u2010UBA) Buenos Aires Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2024,7,3]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0010287"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1038\/srep03894"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1111\/aji.13423"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.immunol.20.100301.064828"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1038\/nri.2017.64"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1095\/biolreprod.103.022640"},{"issue":"12","key":"e_1_2_9_8_1","first-page":"3954","article-title":"Uterine DCs are Crucial for Decidua Formation During Embryo Implantation in Mice","volume":"118","author":"Plaks V.","year":"2008","journal-title":"Journal of Clinical 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