{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,7,30]],"date-time":"2025-07-30T12:26:07Z","timestamp":1753878367558,"version":"3.41.2"},"reference-count":42,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2024,8,23]],"date-time":"2024-08-23T00:00:00Z","timestamp":1724371200000},"content-version":"vor","delay-in-days":22,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["American J Rep Immunol"],"published-print":{"date-parts":[[2024,8]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:sec><jats:title>Introduction<\/jats:title><jats:p>To explore the mechanisms of labor by investigating the autophagy of placental and fetal membranes tissue in normal pregnant women.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Placenta and fetal membranes were collected from women with singleton pregnancies without any medical complications and from women who delivered vaginally (labor\u2010initiated group; L group) or by caesarean section (labor\u2010noninitiated group; NL group). Autophagosomes were observed by transmission electron microscopy (TEM). Immunofluorescence and western blotting (WB) were used to detect protein levels of the autophagy markers LC3A and LC3B. TEM, immunohistochemistry (IHC), and WB were used to compare autophagy in different parts of the placenta and fetal membranes in the L and NL groups. The expression of LC3B\/LC3A, ROCK1, and ROCK2 in the placenta of nonpregnant and pregnant rats was detected by WB and IHC.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>TEM and IHC results showed an increase in the number of autophagosomes and autophagic lysosomes in the L group, and WB results indicated an increase in the LC3B\/A ratio between the placenta and fetal membranes in the L group. Autophagy was significantly increased on the maternal side of the placenta in the L group, and the level of autophagy became higher near rupture in the fetal membranes and near the point where the umbilical cord joins the placenta in the L group. The LC3B\/A ratio increased and ROCK1 and ROCK2 levels decreased in postnatal rats.<\/jats:p><\/jats:sec><jats:sec><jats:title>Discussion<\/jats:title><jats:p>Autophagy can occur in the placenta and fetal membranes and its activity is higher at the onset of labor, suggesting a role in labor.<\/jats:p><\/jats:sec>","DOI":"10.1111\/aji.13903","type":"journal-article","created":{"date-parts":[[2024,8,23]],"date-time":"2024-08-23T11:00:29Z","timestamp":1724410829000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Spatial Activation of Autophagy in Human Placenta\u2010Related Tissue During Labor: A Possible Mechanism for Labor Onset"],"prefix":"10.1111","volume":"92","author":[{"given":"Jing","family":"Shang","sequence":"first","affiliation":[{"name":"Department of Obstetrics and Gynecology Southern Medical University Nanfang Hospital Guangzhou Guangdong China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zhijian","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology Southern Medical University Nanfang Hospital Guangzhou Guangdong China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yingying","family":"Huang","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology Southern Medical University Nanfang Hospital Guangzhou Guangdong China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yuyu","family":"Wu","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology Southern Medical University Nanfang Hospital Guangzhou Guangdong China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6620-2524","authenticated-orcid":false,"given":"Jin","family":"Jin","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology Southern Medical University Nanfang Hospital Guangzhou Guangdong China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2024,8,23]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1113\/expphysiol.2013.076216"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1095\/biolreprod.116.142844"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ajog.2005.08.057"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1582-4934.2010.01069.x"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.1515\/jpm.2010.097"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.1111\/aji.12328"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ajog.2010.02.034"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.1038\/srep17829"},{"issue":"2","key":"e_1_2_10_10_1","doi-asserted-by":"crossref","first-page":"65","DOI":"10.1016\/j.preghy.2013.04.026","article-title":"OP0011. 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