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To do so, RNA Sequencing was performed on chorioallantois and endometrium of mares at 120, 180, 300, and 330 days of gestation (<jats:italic>n<\/jats:italic> = 4\/stage), as well as 45\u2010day chorioallantois (<jats:italic>n<\/jats:italic> = 4) and diestrus endometrium (<jats:italic>n<\/jats:italic> = 3). Expression levels of members of the IL\u20106 cytokine family including ciliary neurotrophic factor (<jats:italic>CNTF<\/jats:italic>), cardiotrophin 1 (<jats:italic>CT\u20101<\/jats:italic>), cardiotrophin\u2010like cytokine factor 1 (<jats:italic>CLCF1<\/jats:italic>), galectin\u201010, oncostatin M (<jats:italic>OSM<\/jats:italic>), and <jats:italic>IL\u20106, \u201011<\/jats:italic>, and \u2010<jats:italic>27<\/jats:italic> were evaluated in addition to the receptors for IL\u20106 (<jats:italic>IL\u20106R<\/jats:italic>) and the common receptor subunit <jats:italic>gp130<\/jats:italic>. Additionally, peripheral concentration of IL\u20106 was assessed.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>In the chorioallantois, differential expression of <jats:italic>IL\u20106, IL\u201011, CNTF, CLCF1, OSM<\/jats:italic>, and <jats:italic>CT\u20101<\/jats:italic> was noted. In the endometrium, the gestational age of pregnancy impacted the expression of <jats:italic>IL\u201011, CNTF<\/jats:italic>, and <jats:italic>CT\u20101<\/jats:italic>. Circulatory IL\u20106 concentrations reached their highest concentrations at 120 days, with lesser concentrations noted at 45, 180, 300, and 330 days. Both <jats:italic>IL\u20106R<\/jats:italic> and <jats:italic>gp130<\/jats:italic> altered in expression throughout equine gestation.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>In conclusion, members of the IL\u20106 cytokine family appear to fluctuate constantly throughout equine pregnancy, with varying expression profiles noted when comparing individual members. Additionally, different expression profiles were noted when comparing chorioallantois, endometrium, and circulation, indicating that the function of the cytokine is tissue\u2010specific.<\/jats:p><\/jats:sec>","DOI":"10.1111\/aji.13910","type":"journal-article","created":{"date-parts":[[2024,7,29]],"date-time":"2024-07-29T12:31:03Z","timestamp":1722256263000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Evaluating the IL\u20106 Family of Cytokines Throughout Equine Gestation"],"prefix":"10.1111","volume":"92","author":[{"given":"Carleigh E.","family":"Fedorka","sequence":"first","affiliation":[{"name":"Department of Animal Sciences Colorado State University  Fort Collins Colorado USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kirsten E.","family":"Scoggin","sequence":"additional","affiliation":[{"name":"Department of Veterinary Sciences University of Kentucky  Lexington Kentucky USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hossam","family":"El\u2010Sheikh Ali","sequence":"additional","affiliation":[{"name":"Department of Veterinary Sciences University of Kentucky  Lexington Kentucky USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mats H. 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