{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T12:34:55Z","timestamp":1790685295326,"version":"4.1.0"},"reference-count":46,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2024,10,29]],"date-time":"2024-10-29T00:00:00Z","timestamp":1730160000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["American J Rep Immunol"],"published-print":{"date-parts":[[2024,11]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:sec><jats:title>Purpose<\/jats:title><jats:p>To gain insight into the endometrial pathophysiology of unexplained repeated implantation failure (RIF), we examined the characteristics of genital tract microbiota and explored the correlation between the microbiota and endometrial receptivity.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Vaginal secretion (VS) and endometrial biopsy (EB) samples were collected from patients with RIF (RIF group, <jats:italic>n<\/jats:italic>\u00a0=\u00a032) and those with infertility who had achieved pregnancy during their initial embryo transfer cycle (control group, <jats:italic>n<\/jats:italic>\u00a0=\u00a018). 16S ribosomal RNA sequencing and quantitative PCR were performed to characterize the microbiota of the two groups. Spearman's correlation analysis was performed to determine the relationship between endometrial receptivity markers and endometrial microbiota.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Endometrial microbiota exhibited distinct characteristics from vaginal microbiota, with a higher alpha\u2010diversity. Alpha\u2010diversity of the endometrial microbiota was higher in the RIF group than in the control group. Compared with the control group, the RIF group had a significant decrease in endometrial <jats:italic>Lactobacillus<\/jats:italic> abundance and an increase in <jats:italic>Gardnerella<\/jats:italic> and <jats:italic>Acinetobacter<\/jats:italic> abundances. The expression levels of endometrial receptivity markers, including homeobox A11, integrin \u03b1v\u03b23, leukemia inhibitor factor, matrix metalloproteinase\u20109, and vascular endothelial growth factor, were lower in the RIF group than in the control group. Moreover, the expression levels of these markers were correlated with endometrial <jats:italic>Lactobacillus, Gardnerella<\/jats:italic>, and <jats:italic>Acinetobacter<\/jats:italic> abundances.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>RIF is characterized by endometrial microbiota dysbiosis and poor endometrial receptivity. Moreover, abnormal endometrial microbiota is associated with impaired endometrial receptivity, which may be a potential cause of unexplained RIF.<\/jats:p><\/jats:sec>","DOI":"10.1111\/aji.70005","type":"journal-article","created":{"date-parts":[[2024,10,29]],"date-time":"2024-10-29T08:42:02Z","timestamp":1730191322000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["Altered Endometrial Microbiota Profile Is Associated With Poor Endometrial Receptivity of Repeated Implantation Failure"],"prefix":"10.1111","volume":"92","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-7356-8404","authenticated-orcid":false,"given":"Rongxue","family":"Zhang","sequence":"first","affiliation":[{"name":"Department of Reproductive Medicine Huai'an Maternal and Child Health Care Center  Huai'an Jiangsu People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Miaomiao","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Reproductive Medicine Huai'an Maternal and Child Health Care Center  Huai'an Jiangsu People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jixiang","family":"Zhong","sequence":"additional","affiliation":[{"name":"Department of Reproductive Medicine Huai'an Maternal and Child Health Care Center  Huai'an Jiangsu People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Huiying","family":"Xue","sequence":"additional","affiliation":[{"name":"Department of Reproductive Medicine Huai'an Maternal and Child Health Care Center  Huai'an Jiangsu People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2024,10,29]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.fertnstert.2012.03.010"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1093\/humupd\/dml004"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00404-015-3954-0"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1093\/humrep\/dez120"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10815-015-0614-z"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1186\/s40168-021-01184-w"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ajog.2016.09.075"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1155\/2019\/4893437"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jri.2022.103634"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1002\/rmb2.12389"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00404-022-06755-2"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.3390\/jcm10184063"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1128\/mSystems.00777-19"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.3390\/jcm11092481"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2021.748447"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.fertnstert.2003.07.023"},{"key":"e_1_2_9_18_1","first-page":"121","volume-title":"Preembryo Grading","author":"Veeck L. 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