{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,9,10]],"date-time":"2025-09-10T23:50:36Z","timestamp":1757548236815,"version":"3.41.2"},"reference-count":89,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2024,11,22]],"date-time":"2024-11-22T00:00:00Z","timestamp":1732233600000},"content-version":"vor","delay-in-days":21,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100007686","name":"Instituto Nacional de Perinatolog\u00eda","doi-asserted-by":"publisher","award":["2019\u20101\u20105 to VZC"],"award-info":[{"award-number":["2019\u20101\u20105 to VZC"]}],"id":[{"id":"10.13039\/501100007686","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["American J Rep Immunol"],"published-print":{"date-parts":[[2024,11]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:sec><jats:title>Problem<\/jats:title><jats:p>Intrauterine infection is one of the most jeopardizing conditions associated with adverse outcomes, including preterm birth; however, multiple tolerance mechanisms operate at the maternal\u2013fetal interface to avoid the rejection of the fetus. Among the factors that maintain the uterus as an immunoprivileged site, Galectin\u20101 (Gal\u20101), an immunomodulatory glycan\u2010binding protein secreted by the maternal\u2010fetal unit, is pivotal in promoting immune cell homeostasis. This work aimed to evaluate the role of Gal\u20101 during a lipopolysaccharide\u00a0(LPS)\u2010induced\u2010inflammatory milieu.<\/jats:p><\/jats:sec><jats:sec><jats:title>Method of Study<\/jats:title><jats:p>Using an ex vivo culture with two independent compartments, human fetal membranes at term were pretreated with 40 and 80\u00a0ng\/mL of Gal\u20101, then to reproduce an intraamniotic inflammation, the fetal side of membranes was stimulated with 500\u00a0ng\/mL of LPS for 24\u00a0h. The concentrations of tumor necrosis factor (TNF)\u2010\u03b1, interleukin (IL)\u20101\u03b2, IL\u20106, monocyte chemoattractant protein (MCP1), macrophage inflammatory protein (MIP1) \u03b1, regulated upon activation normal T cell expressed and secreted (RANTES), and matrix metalloproteinase (MMP)\u20109 were measured in both amnion and choriodecidua compartments.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>In a tissue\u2010specific fashion profile, pretreatment with the physiologic concentration of Gal\u20101 significantly diminished the LPS\u2010dependent secretion of TNF\u2010\u03b1, IL\u20101\u03b2, Il\u20106, MCP1, MIP1\u03b1, RANTES, and MMP\u20109.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Gal\u20101 elicits an anti\u2010inflammatory effect on the human fetal membranes stimulated with LPS, which supports the hypothesis that Gal\u20101 is part of the immunomodulatory mechanisms intended to stop the harmful effect of inflammation of the maternal\u2013fetal interface.<\/jats:p><\/jats:sec>","DOI":"10.1111\/aji.70016","type":"journal-article","created":{"date-parts":[[2024,11,22]],"date-time":"2024-11-22T09:29:05Z","timestamp":1732267745000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Galectin\u20101 Elicits a Tissue\u2010Specific Anti\u2010Inflammatory and Anti\u2010Degradative Effect Upon LPS\u2010Induced Response in an Ex Vivo Model of Human Fetal Membranes Modeling an Intraamniotic Inflammation"],"prefix":"10.1111","volume":"92","author":[{"given":"Jazmin","family":"Hern\u00e1ndez\u2010Rodr\u00edguez","sequence":"first","affiliation":[{"name":"Departamento de Inmunobioqu\u00edmica Instituto Nacional de Perinatolog\u00eda  Ciudad de M\u00e9xico Mexico"},{"name":"Laboratorio de Patolog\u00eda Experimental UME Unidad de Medicina Experimental Facultad de Medicina UNAM Ciudad Universitaria  Ciudad de M\u00e9xico 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