{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,28]],"date-time":"2026-09-28T13:38:17Z","timestamp":1790602697483,"version":"4.1.0"},"reference-count":44,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2025,5,15]],"date-time":"2025-05-15T00:00:00Z","timestamp":1747267200000},"content-version":"vor","delay-in-days":14,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82460304"],"award-info":[{"award-number":["82460304"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004772","name":"Natural Science Foundation of Ningxia Province","doi-asserted-by":"publisher","award":["2023AAC03589"],"award-info":[{"award-number":["2023AAC03589"]}],"id":[{"id":"10.13039\/501100004772","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["American J Rep Immunol"],"published-print":{"date-parts":[[2025,5]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Endometriosis (EMs), characterized by ectopic endometrial growth causing infertility. Endoplasmic reticulum stress (ERS) is an important cellular defense mechanism. However, the correlation between ERS and EMs remains unclear. We aimed to investigate the relationship between them, identify biomarkers, and offer new insights into the treatment of EMs.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Two RNA expression datasets (GSE120103 and GSE25628) related to EMs in <jats:italic>Homo sapiens<\/jats:italic> were used to identify ERS\u2010differentially expressed genes (ERS\u2010DEGs). Protein\u2013protein interaction (PPI) networks and CytoHubba (Cytoscape) identified ERS\u2010associated HUB genes, with receiver operating characteristic curves (ROC) evaluating diagnostic value. Constructed mRNA\u2010microRNA (miRNA)\/RNA\u2010transcription factor (TF) interaction networks and performed ssGSEA to compare immune infiltration between EMs patients and controls. Real\u2010time quantitative polymerase chain reaction (RT\u2010qPCR), western blotting (WB), and immunohistochemistry (IHC) were performed to assess potential biomarker levels.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Thirty\u2010three ERS\u2010DEGs were identified, with nine HUB genes (<jats:italic>HSPA5, XBP1, HSP90B1, DNAJC3, PDIA3, PDIA6, PDIA4, HERPUD1<\/jats:italic>, and <jats:italic>MANF<\/jats:italic>) demonstrating diagnostic efficacy (AUC\u00a0&gt;\u00a00.7). Furthermore, immune infiltration revealed a significant relationship between immune cell abundance and HUB gene expression. Experimental validation confirmed the consistency of four biomarkers (<jats:italic>HSPA5, HSP90B1, PDIA6<\/jats:italic>, and <jats:italic>HERPUD1<\/jats:italic>). Regulatory network analysis identified 62 miRNAs and 44 TFs interacting with HUB genes, suggesting a multifactorial immunometabolic axis.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>We identified four biomarkers (<jats:italic>HSPA5, HSP90B1, PDIA6<\/jats:italic>, and <jats:italic>HERPUD1<\/jats:italic>) associated with ERS that offer new insights into the detection and treatment of EMs. Our findings indicate an abnormal response to ERS and immune system infiltration contribute to the progression of EMs.<\/jats:p><\/jats:sec>","DOI":"10.1111\/aji.70092","type":"journal-article","created":{"date-parts":[[2025,5,15]],"date-time":"2025-05-15T10:26:46Z","timestamp":1747304806000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Deciphering the Molecular Crosstalk of Endoplasmic Reticulum Stress and Immune Infiltration in Endometriosis"],"prefix":"10.1111","volume":"93","author":[{"ORCID":"https:\/\/orcid.org\/0009-0004-0113-3224","authenticated-orcid":false,"given":"Yuan","family":"Ma","sequence":"first","affiliation":[{"name":"Ningxia Medical University  Yinchuan China"},{"name":"General Hospital of Ningxia Medical University  Yinchuan Ningxia China"},{"name":"Key Laboratory of Fertility Preservation &amp; Maintenance of Ministry of Education, Ningxia Medical University  Yinchuan Ningxia China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chunfang","family":"Ha","sequence":"additional","affiliation":[{"name":"Ningxia Medical University  Yinchuan China"},{"name":"General Hospital of Ningxia Medical University  Yinchuan Ningxia China"},{"name":"Key Laboratory of Fertility Preservation &amp; Maintenance of Ministry of Education, Ningxia Medical University  Yinchuan Ningxia China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ruyue","family":"Li","sequence":"additional","affiliation":[{"name":"General Hospital of Ningxia Medical University  Yinchuan Ningxia China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ruiqi","family":"Zhang","sequence":"additional","affiliation":[{"name":"Ningxia Medical University  Yinchuan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Min","family":"Li","sequence":"additional","affiliation":[{"name":"Ningxia Medical University  Yinchuan China"},{"name":"General Hospital of Ningxia Medical University  Yinchuan Ningxia China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,5,15]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1093\/hropen\/hoac009"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1097\/GRF.0000000000000320"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.molcel.2017.06.017"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.3390\/ph15040401"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biocel.2017.04.001"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.3389\/fnmol.2017.00203"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1177\/1933719108324893"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.semcancer.2019.07.009"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1093\/humupd\/dmt010"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.1095\/biolreprod.110.089532"},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ajpath.2018.06.011"},{"key":"e_1_2_6_13_1","doi-asserted-by":"publisher","DOI":"10.1093\/molehr\/gaab002"},{"key":"e_1_2_6_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.molmed.2018.07.004"},{"key":"e_1_2_6_15_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkl887"},{"key":"e_1_2_6_16_1","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btm254"},{"key":"e_1_2_6_17_1","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/bts034"},{"key":"e_1_2_6_18_1","doi-asserted-by":"publisher","DOI":"10.3389\/fcell.2021.619396"},{"key":"e_1_2_6_19_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gku1179"},{"key":"e_1_2_6_20_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0506580102"},{"key":"e_1_2_6_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cels.2015.12.004"},{"key":"e_1_2_6_22_1","doi-asserted-by":"publisher","DOI":"10.3389\/fonc.2020.607622"},{"key":"e_1_2_6_23_1","doi-asserted-by":"publisher","DOI":"10.1097\/JTO.0b013e3181ec173d"},{"key":"e_1_2_6_24_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkz757"},{"key":"e_1_2_6_25_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkac1067"},{"key":"e_1_2_6_26_1","doi-asserted-by":"crossref","DOI":"10.1136\/bmj-2022-070750","article-title":"Pathophysiology, Diagnosis, and Management of Endometriosis","volume":"379","author":"Horne A. 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