{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,1]],"date-time":"2026-07-01T19:36:39Z","timestamp":1782934599201,"version":"3.54.5"},"reference-count":73,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2025,9,9]],"date-time":"2025-09-09T00:00:00Z","timestamp":1757376000000},"content-version":"vor","delay-in-days":8,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100004735","name":"Natural Science Foundation of Hunan Province","doi-asserted-by":"publisher","award":["2022JJ30802"],"award-info":[{"award-number":["2022JJ30802"]}],"id":[{"id":"10.13039\/501100004735","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["American J Rep Immunol"],"published-print":{"date-parts":[[2025,9]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:sec><jats:title>Problem<\/jats:title><jats:p>Preeclampsia (PE) is a leading cause of perinatal maternal and fetal mortality. Clinical and pathological studies suggest that placental and decidual cell dysfunction may contribute to this condition. However, the pathogenesis of PE remains poorly understood. Therefore, this study aims to investigate the heterogeneous changes in cell types within placental and decidual tissue isolated from cesarean sections using single\u2010cell sequencing.<\/jats:p><\/jats:sec><jats:sec><jats:title>Method of Study<\/jats:title><jats:p>Patients included those diagnosed with PE (<jats:italic>n<\/jats:italic>\u00a0=\u00a03) and normal pregnancy (NP) (<jats:italic>n<\/jats:italic>\u00a0=\u00a03). Overall, 32\u00a0279 cells (PE: 16.575; NP: 15\u00a0704) were identified across nine cell types, including villous cytotrophoblast (VCT), syncytiotrophoblast (SCT), extravillous trophoblasts (EVT), endothelial cells, neutrophil, Hofbauer cells, T cells, dendritic cells (DC), macrophages, fibroblasts, and B cells. VCT and T cells subclusters and pseudotime were analyzed. The in vivo and in vitro experiments are focused on the invasion ability of EVT.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Using gene set variation analysis (GSVA) for differential expression genes, cell\u2010reclustering, and pseudotime analysis, the VCT in patients with PE showed a tendency to differentiate toward SCT instead of EVT. And the invasive ability of PE EVT cells was declined by decreased expression of the invasion\u2010related gene TMEM200A. Additionally, the impaired immune environment of T\u2010cell differentiation into CD8+T cells instead of Treg cells is the main change related to PE.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>These findings suggest that understanding how T cell differentiation occurs in the early stage of pregnancy and how the predominantly CD8+T cell\u2010driven immune environment influences VCT differentiation are crucial for elucidating the pathogenesis of PE.<\/jats:p><\/jats:sec>","DOI":"10.1111\/aji.70101","type":"journal-article","created":{"date-parts":[[2025,9,9]],"date-time":"2025-09-09T18:14:12Z","timestamp":1757441652000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Maternal\u2010Fetal Interface Cell Dysfunction in Patients With Preeclampsia Revealed via Single\u2010Cell RNA Sequencing"],"prefix":"10.1111","volume":"94","author":[{"given":"Songyuan","family":"Xiao","sequence":"first","affiliation":[{"name":"Department of Obstetrics and Gynecology Second XiangYa Hospital of Central South University  Changsha Hunan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yiling","family":"Ding","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology Second XiangYa Hospital of Central South University  Changsha Hunan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ling","family":"Yu","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology Second XiangYa Hospital of Central South University  Changsha Hunan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yali","family":"Deng","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology Second XiangYa Hospital of Central South University  Changsha Hunan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yang","family":"Zhou","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology Second XiangYa Hospital of Central South University  Changsha Hunan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mei","family":"Peng","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology Second XiangYa Hospital of Central South University  Changsha Hunan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Weisi","family":"Lai","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology Second XiangYa Hospital of Central South University  Changsha Hunan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yanting","family":"Nie","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology Second XiangYa Hospital of Central South University  Changsha Hunan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1392-9495","authenticated-orcid":false,"given":"Wen","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology Second XiangYa Hospital of Central South University  Changsha Hunan China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,9,9]]},"reference":[{"key":"e_1_2_12_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(20)32335-7"},{"key":"e_1_2_12_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jogc.2022.03.002"},{"key":"e_1_2_12_4_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41572-023-00417-6"},{"key":"e_1_2_12_5_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41581-019-0119-6"},{"key":"e_1_2_12_6_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41586-023-05869-0"},{"key":"e_1_2_12_7_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41422-018-0066-y"},{"key":"e_1_2_12_8_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41421-020-00236-z"},{"key":"e_1_2_12_9_1","doi-asserted-by":"publisher","DOI":"10.1038\/nri.2017.64"},{"key":"e_1_2_12_10_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41586-018-0698-6"},{"key":"e_1_2_12_11_1","doi-asserted-by":"publisher","DOI":"10.1530\/REP-20-0231"},{"key":"e_1_2_12_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ajog.2011.06.023"},{"key":"e_1_2_12_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.apsb.2022.08.005"},{"key":"e_1_2_12_14_1","doi-asserted-by":"publisher","DOI":"10.1002\/gcc.20659"},{"key":"e_1_2_12_15_1","doi-asserted-by":"publisher","DOI":"10.3389\/fphys.2022.1055234"},{"key":"e_1_2_12_16_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12987-023-00424-5"},{"key":"e_1_2_12_17_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0602208103"},{"key":"e_1_2_12_18_1","doi-asserted-by":"publisher","DOI":"10.1101\/gad.346405"},{"key":"e_1_2_12_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.placenta.2011.12.020"},{"key":"e_1_2_12_20_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood.2019003691"},{"key":"e_1_2_12_21_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.abn3027"},{"key":"e_1_2_12_22_1","doi-asserted-by":"publisher","DOI":"10.1111\/cas.15307"},{"key":"e_1_2_12_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.immuni.2023.07.019"},{"key":"e_1_2_12_24_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41586-023-05718-0"},{"key":"e_1_2_12_25_1","doi-asserted-by":"publisher","DOI":"10.1038\/nm.3890"},{"key":"e_1_2_12_26_1","doi-asserted-by":"publisher","DOI":"10.3389\/fgene.2022.799224"},{"key":"e_1_2_12_27_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12935-023-02983-x"},{"key":"e_1_2_12_28_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcb.29948"},{"key":"e_1_2_12_29_1","first-page":"4418","article-title":"Construction and Experimental Validation of a Necroptosis\u2010Related lncRNA Signature as a Prognostic Model and Immune\u2010Landscape Predictor for Lung Adenocarcinoma","volume":"13","author":"Zhang T.","year":"2023","journal-title":"American Journal of Cancer Research"},{"key":"e_1_2_12_30_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41416-023-02140-1"},{"key":"e_1_2_12_31_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00018-023-04892-8"},{"key":"e_1_2_12_32_1","doi-asserted-by":"publisher","DOI":"10.3324\/haematol.2021.278436"},{"key":"e_1_2_12_33_1","doi-asserted-by":"publisher","DOI":"10.1111\/jcpe.12930"},{"key":"e_1_2_12_34_1","doi-asserted-by":"publisher","DOI":"10.3390\/diagnostics12040919"},{"key":"e_1_2_12_35_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.molcel.2018.08.014"},{"key":"e_1_2_12_36_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cjca.2023.07.011"},{"key":"e_1_2_12_37_1","doi-asserted-by":"publisher","DOI":"10.1002\/jgm.3416"},{"key":"e_1_2_12_38_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.2200433"},{"key":"e_1_2_12_39_1","doi-asserted-by":"publisher","DOI":"10.3390\/ph17050605"},{"key":"e_1_2_12_40_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.immuni.2016.10.008"},{"key":"e_1_2_12_41_1","doi-asserted-by":"publisher","DOI":"10.1093\/hmg\/ddaa077"},{"key":"e_1_2_12_42_1","doi-asserted-by":"publisher","DOI":"10.1155\/2013\/423965"},{"key":"e_1_2_12_43_1","doi-asserted-by":"publisher","DOI":"10.3390\/jcm10020351"},{"key":"e_1_2_12_44_1","doi-asserted-by":"publisher","DOI":"10.1155\/2022\/7346460"},{"key":"e_1_2_12_45_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41586-022-04717-x"},{"key":"e_1_2_12_46_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12072-023-10608-8"},{"key":"e_1_2_12_47_1","doi-asserted-by":"publisher","DOI":"10.4161\/21624011.2014.955709"},{"key":"e_1_2_12_48_1","doi-asserted-by":"publisher","DOI":"10.1080\/19420862.2017.1395541"},{"key":"e_1_2_12_49_1","doi-asserted-by":"publisher","DOI":"10.1002\/1873-3468.14468"},{"key":"e_1_2_12_50_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1229620"},{"key":"e_1_2_12_51_1","doi-asserted-by":"publisher","DOI":"10.1084\/jem.20132038"},{"key":"e_1_2_12_52_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41586-023-05912-0"},{"key":"e_1_2_12_53_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2023.1174406"},{"key":"e_1_2_12_54_1","doi-asserted-by":"publisher","DOI":"10.1084\/jem.20210108"},{"key":"e_1_2_12_55_1","doi-asserted-by":"publisher","DOI":"10.1038\/ni.3456"},{"key":"e_1_2_12_56_1","doi-asserted-by":"publisher","DOI":"10.1084\/jem.20211112"},{"key":"e_1_2_12_57_1","doi-asserted-by":"publisher","DOI":"10.1242\/dmm.045377"},{"key":"e_1_2_12_58_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.abe6474"},{"key":"e_1_2_12_59_1","doi-asserted-by":"publisher","DOI":"10.1161\/CIRCRESAHA.118.313276"},{"key":"e_1_2_12_60_1","doi-asserted-by":"publisher","DOI":"10.1111\/sji.12756"},{"key":"e_1_2_12_61_1","doi-asserted-by":"publisher","DOI":"10.14348\/molcells.2021.0211"},{"key":"e_1_2_12_62_1","doi-asserted-by":"publisher","DOI":"10.1002\/mgg3.1730"},{"key":"e_1_2_12_63_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2024.1401738"},{"key":"e_1_2_12_64_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.molimm.2023.07.005"},{"key":"e_1_2_12_65_1","doi-asserted-by":"publisher","DOI":"10.1111\/aji.12647"},{"key":"e_1_2_12_66_1","doi-asserted-by":"publisher","DOI":"10.1113\/JP279532"},{"issue":"0","key":"e_1_2_12_67_1","first-page":"1082","article-title":"Analysis of TCR Repertoire and PD\u20101 Expression in Decidual and Peripheral CD8(+) T Cells Reveals Distinct Immune Mechanisms in Miscarriage and Preeclampsia","volume":"11","author":"Keiko M.","year":"2020","journal-title":"Frontiers in Immunology"},{"key":"e_1_2_12_68_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2021.791606"},{"key":"e_1_2_12_69_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ajog.2020.09.026"},{"key":"e_1_2_12_70_1","doi-asserted-by":"publisher","DOI":"10.1001\/jama.2022.24093"},{"key":"e_1_2_12_71_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jacc.2021.10.033"},{"key":"e_1_2_12_72_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10554-015-0694-y"},{"key":"e_1_2_12_73_1","doi-asserted-by":"publisher","DOI":"10.3390\/biomedicines11082328"},{"key":"e_1_2_12_74_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(23)02128-1"}],"container-title":["American Journal of Reproductive Immunology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/aji.70101","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,9,22]],"date-time":"2025-09-22T13:02:47Z","timestamp":1758546167000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/aji.70101"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,9]]},"references-count":73,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2025,9]]}},"alternative-id":["10.1111\/aji.70101"],"URL":"https:\/\/doi.org\/10.1111\/aji.70101","archive":["Portico"],"relation":{},"ISSN":["1046-7408","1600-0897"],"issn-type":[{"value":"1046-7408","type":"print"},{"value":"1600-0897","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,9]]},"assertion":[{"value":"2024-08-05","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-05-14","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-09-09","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e70101"}}