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Inflammation alters stem cell factors and cell transitions, disrupting immune balance and leading to adverse outcomes.<\/jats:p><\/jats:sec><jats:sec><jats:title>Objective<\/jats:title><jats:p>This study explored the effects of the pro\u2010inflammatory cytokine TNF\u2010\u03b1 on Amnion epithelial cells (AECs), focusing on stem cell transcription factors (TFs), senescence, and the epithelial\u2010mesenchymal transition (EMT).<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Transcriptomic data were generated to compare the stem cell TFs expression (KLF4, c\u2010MYC, OCT\u20104, SOX2, NANOG) between the placenta and fetal membrane (FM); AEC were treated with 50\u00a0ng\/mL TNF\u2010\u03b1 for 48\u00a0h. Gene expression of stem cell TFs was assessed\u00a0using qPCR; p38 MAPK activation and differential expression of KLF4, c\u2010MYC were subsequently verified by Western blotting. Cellular senescence\u00a0was evaluated using SA\u2010\u03b2\u2010Gal staining, and the pro\u2010inflammatory\u00a0cytokines IL\u20106 and IL\u20108\u00a0were measured\u00a0using ELISA. The EMT\u00a0was determined\u00a0by measuring cell shape index and vimentin\/CK\u201018 immunofluorescence.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>The placenta and the FM expressed higher mRNA levels of KLF4 than c\u2010MYC, SOX2, OCT\u20104, and NANOG. AEC exhibited significantly higher KLF4 and c\u2010MYC (<jats:italic>p<\/jats:italic>\u00a0&lt;\u00a00.05). KLF4 downregulation (<jats:italic>p<\/jats:italic>\u00a0&lt;\u00a00.05) and an increase in c\u2010MYC (<jats:italic>p<\/jats:italic>\u00a0&lt;\u00a00.01) in mRNA and protein levels were observed.\u00a0The p38 MAPK activation (<jats:italic>p<\/jats:italic>\u00a0&lt;\u00a00.01) increased cellular senescence (<jats:italic>p<\/jats:italic>\u00a0&lt;\u00a00.05) and increased IL\u20106, IL\u20108 production (<jats:italic>p<\/jats:italic>\u00a0&lt;\u00a00.01) with no change in cellular transition.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>This study shows how TNF\u2010\u03b1 affects stem cell TFs and impacts cellular integrity, senescence, and inflammatory responses, thus influencing adverse pregnancy outcomes.<\/jats:p><\/jats:sec>","DOI":"10.1111\/aji.70106","type":"journal-article","created":{"date-parts":[[2025,6,2]],"date-time":"2025-06-02T12:55:31Z","timestamp":1748868931000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["The Impacts of TNF\u2010\u03b1\u2010Induced Inflammation on Amnion Epithelial Cells: Exploring Stem Cell Gene Expression, Senescence, Inflammatory Responses, and Cellular Transition"],"prefix":"10.1111","volume":"93","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-8542-2218","authenticated-orcid":false,"given":"Madhuri","family":"Tatiparthy","sequence":"first","affiliation":[{"name":"Division of Basic Science and Translational Research Department of Obstetrics &amp; Gynecology The University of Texas Medical Branch at Galveston  Texas 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