{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,9]],"date-time":"2026-10-09T17:45:11Z","timestamp":1791567911890,"version":"4.3.4"},"reference-count":69,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2025,10,7]],"date-time":"2025-10-07T00:00:00Z","timestamp":1759795200000},"content-version":"vor","delay-in-days":6,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100005847","name":"Health and Medical Research Fund","doi-asserted-by":"publisher","award":["10210956"],"award-info":[{"award-number":["10210956"]}],"id":[{"id":"10.13039\/501100005847","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["American J Rep Immunol"],"published-print":{"date-parts":[[2025,10]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Research Question<\/jats:title>\n                    <jats:p>For women with reproductive failure, prednisone is widely used but remains controversial as a therapy for improving clinical outcomes. This study aimed to investigate the impact of prednisone on various endometrial immune cells.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Design<\/jats:title>\n                    <jats:p>A total of 24 women with repeated implantation failure and recurrent miscarriage underwent their first endometrial biopsy precisely 7 days after luteinizing hormone surge (LH surge +7). Prednisone was administered from the first day of the subsequent menstrual cycle, followed by the second endometrial sampling on day LH surge +7 again. The density and cell\u2013cell clustering of CD3\u2212CD56+CD16\u2212 NK cells, CD3\u2212CD56+CD16+ NK cells, CD68+CD16\u2212 macrophages, CD68+CD16+ macrophages, CD3+CD56\u2212 T cells, and CD3+CD56+ NK\u2010T cells were analyzed by multiplex staining and compared before and after prednisone treatment.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      Following prednisone treatment, a significant decrease in the percentage of CD3\u2212CD56+CD16\u2212 NK cells (\n                      <jats:italic>p &lt;<\/jats:italic>\n                      0.001), CD68+CD16\u2212 macrophages (\n                      <jats:italic>p<\/jats:italic>\n                      = 0.007), and their clustering degree (\n                      <jats:italic>p<\/jats:italic>\n                      = 0.038) was observed. No significant changes were noted in CD3\u2212CD56+CD16+ NK cells, CD68+CD16+ macrophages, CD3+CD56\u2212 T cells, CD3+CD56+ NK\u2010T cells, and their cell\u2013cell clustering.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>Prednisone does not generally reduce all endometrial immune cells; instead, it selectively suppresses specific immune cell subtypes in women with reproductive failure.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/aji.70151","type":"journal-article","created":{"date-parts":[[2025,10,8]],"date-time":"2025-10-08T00:17:13Z","timestamp":1759882633000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Distinct Suppression of Prednisone on Endometrial Immune Cells in Women With Reproductive 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