{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,5]],"date-time":"2026-09-05T15:55:04Z","timestamp":1788623704132,"version":"build-2803163510"},"reference-count":40,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2025,9,7]],"date-time":"2025-09-07T00:00:00Z","timestamp":1757203200000},"content-version":"vor","delay-in-days":6,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100003710","name":"Korea Health Industry Development Institute","doi-asserted-by":"publisher","award":["HI21C1770"],"award-info":[{"award-number":["HI21C1770"]}],"id":[{"id":"10.13039\/501100003710","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003725","name":"National Research Foundation of Korea","doi-asserted-by":"publisher","award":["2020R1I1A1A0106783712"],"award-info":[{"award-number":["2020R1I1A1A0106783712"]}],"id":[{"id":"10.13039\/501100003725","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["American J Rep Immunol"],"published-print":{"date-parts":[[2025,9]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:sec><jats:title>Objective<\/jats:title><jats:p>Exosomes are secreted by most cell types and reflect the internal state of their cells of origin, playing crucial roles in the progression of various pathological conditions. Endometriosis is a chronic, estrogen\u2010dependent inflammatory disease characterized by the ectopic presence of endometrial\u2010like tissue outside the uterus, including in the ovaries, fallopian tubes, and peritoneal cavity. It primarily affects women of reproductive age and is often associated with infertility. Despite its high prevalence, the underlying pathogenesis remains poorly understood, and the high recurrence rate highlights the urgent need for reliable, non\u2010invasive biomarkers for early diagnosis. Although exosomes have been implicated in the pathophysiology of endometriosis\u2014particularly in processes such as angiogenesis and immune modulation\u2014their role in regulating cell proliferation and apoptosis remains poorly understood.<\/jats:p><\/jats:sec><jats:sec><jats:title>Method of Study<\/jats:title><jats:p>We collected eutopic endometrial tissues from patients with endometriosis (EMS\u2010EM) and without endometriosis (CTL\u2010EM) and cultured to primary cells. Exosomes were extracted from EMS\u2010EM and CTL\u2010EM, respectively, and then treated the EMS\u2010EM cells for 24\u00a0h.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>CCK\u20108 and FACS assays showed that exosomes extracted from EMS\u2010EM (EMS\u2010Exo) treatment induced cell proliferation and reduced apoptosis. In particular, JC\u20101 red\/green dye was significantly increased, and the mitochondrial apoptosis signal pathway was regulated by EMS\u2010Exo treatment. The examination of protein expression identified that proliferation was induced through ERK and AKT signaling, and the mitochondrial apoptosis signaling pathway was regulated through the PI3K\/AKT mechanism. miRNA array analysis showed differences in 16 miRNAs, with miR\u2010200a\u20103p upregulated and 29a\u20103p downregulated. qRT\u2010PCR was performed on serum exosomes for in vivo diagnosis, and significant differences were observed in five miRNAs. Through ROC curve analysis, the combination of three miRNAs was derived to show the highest diagnostic performance.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>These findings suggest that the exosome\u2010related mechanism influences the development of endometriosis and highlights the potential of exosomes as novel diagnostic biomarkers for endometriosis.<\/jats:p><\/jats:sec>","DOI":"10.1111\/aji.70155","type":"journal-article","created":{"date-parts":[[2025,9,7]],"date-time":"2025-09-07T16:39:43Z","timestamp":1757263183000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Effects of Exosomes Derived From Eutopic Endometrial Cells in Endometriosis and the Discovery of Related Serum miRNA Biomarkers for Endometriosis"],"prefix":"10.1111","volume":"94","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-4869-6281","authenticated-orcid":false,"given":"Inha","family":"Lee","sequence":"first","affiliation":[{"name":"Department of Obstetrics and Gynecology Gangnam Severance Hospital Yonsei University College of Medicine  Seoul Republic of Korea"},{"name":"Institute of Women's Life Medical Science Yonsei University College of Medicine  Seoul Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"SeHee","family":"Kim","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology Gangnam Severance Hospital Yonsei University College of Medicine  Seoul Republic of Korea"},{"name":"Department of Medical Device Engineering and Management Yonsei University College of Medicine  Seoul Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gee 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