{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,19]],"date-time":"2026-10-19T05:41:03Z","timestamp":1792388463765,"version":"4.4.0"},"reference-count":25,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2025,11,18]],"date-time":"2025-11-18T00:00:00Z","timestamp":1763424000000},"content-version":"vor","delay-in-days":17,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["American J Rep Immunol"],"published-print":{"date-parts":[[2025,11]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>This study aimed to characterize the endometrial microbiota in patients with chronic endometritis (CE) after doxycycline treatment and to compare microbial profiles and pregnancy outcomes between treated CE patients and those without CE.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>Fifty\u2010two women undergoing frozen\u2013thawed embryo transfer were enrolled. Based on histopathological findings before oocyte retrieval, patients were categorized into the CE treatment group or the non\u2010CE group. CE patients received doxycycline (100\u00a0mg twice daily for 14 days). Endometrial tissue was collected 1 day before endometrial transformation and analyzed by 16S rRNA gene sequencing of the V3\u2013V4 region using QIIME2. Clinical and microbiological outcomes were compared between the groups.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      The two groups showed no significant differences in age, body mass index, endometrial thickness, number of embryos transferred, or transfer protocol (\n                      <jats:italic>p<\/jats:italic>\n                      &gt; 0.05). Infertility duration was significantly longer in the CE group (\n                      <jats:italic>p<\/jats:italic>\n                      &lt; 0.05). Alpha\u2010diversity analysis revealed lower microbial richness and diversity in the CE group (\n                      <jats:italic>p<\/jats:italic>\n                      &lt; 0.05). Firmicutes abundance was significantly reduced (\n                      <jats:italic>p<\/jats:italic>\n                      = 0.0214), whereas\n                      <jats:italic>Bacteroidetes<\/jats:italic>\n                      and\n                      <jats:italic>Planctomycetes<\/jats:italic>\n                      were increased (\n                      <jats:italic>p<\/jats:italic>\n                      &lt; 0.05). At the genus level,\n                      <jats:italic>Lactobacillus<\/jats:italic>\n                      remained dominant but was markedly decreased in the CE group (\n                      <jats:italic>p<\/jats:italic>\n                      = 0.0157), while\n                      <jats:italic>Ralstonia<\/jats:italic>\n                      and\n                      <jats:italic>Methylobacterium<\/jats:italic>\n                      were enriched (\n                      <jats:italic>p<\/jats:italic>\n                      &lt; 0.05). The clinical pregnancy rate and biochemical pregnancy rate showed no significant differences between the groups (\n                      <jats:italic>p<\/jats:italic>\n                      &gt; 0.05), but the live birth rate was significantly lower in the CE group (\n                      <jats:italic>p<\/jats:italic>\n                      = 0.036).\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>\n                      Despite histological resolution following doxycycline therapy, CE patients exhibited persistent dysbiosis characterized by decreased\n                      <jats:italic>Lactobacillus<\/jats:italic>\n                      and increased opportunistic taxa, which may impair endometrial receptivity and pregnancy outcomes. These findings suggest that antibiotic therapy alone may not fully restore microbial balance in CE, warranting further investigation into microbiota\u2010targeted interventions to improve reproductive success.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/aji.70184","type":"journal-article","created":{"date-parts":[[2025,11,18]],"date-time":"2025-11-18T11:52:02Z","timestamp":1763466722000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Study on the Effects of Doxycycline Treatment on Endometrial Microbiota and Pregnancy Outcomes in Chronic Endometritis"],"prefix":"10.1111","volume":"94","author":[{"given":"Huanhuan","family":"Guo","sequence":"first","affiliation":[{"name":"Reproductive Medicine Center of Affiliated Hospital of Putian University  Putuian Fujian China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lili","family":"Chen","sequence":"additional","affiliation":[{"name":"Reproductive Medicine Center of Affiliated Hospital of Putian University  Putuian Fujian China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lan","family":"Liu","sequence":"additional","affiliation":[{"name":"Reproductive Medicine Center of Affiliated Hospital of Putian University  Putuian Fujian China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,11,18]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.48095\/cccg2024230"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12905-024-03245-2"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1111\/aji.12438"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2021.748447"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.fertnstert.2016.11.028"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.1111\/jog.13937"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ajog.2018.02.012"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.fertnstert.2019.05.015"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-019-46173-0"},{"key":"e_1_2_10_11_1","doi-asserted-by":"publisher","DOI":"10.1007\/s40618-022-01752-3"},{"key":"e_1_2_10_12_1","doi-asserted-by":"publisher","DOI":"10.3390\/microorganisms13010197"},{"key":"e_1_2_10_13_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12905-021-01239-y"},{"key":"e_1_2_10_14_1","doi-asserted-by":"publisher","DOI":"10.1111\/aji.13369"},{"issue":"4","key":"e_1_2_10_15_1","first-page":"882","article-title":"Electronic Address, A.a.o. and M. 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