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T\u2010bet (Tbx21) is a key transcription factor shaping NK cell effector functions, yet its role in decidual NK (dNK) cell adaptation across gestation remains unclear.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Method of Study<\/jats:title>\n                    <jats:p>\n                      We used a T\u2010bet fate\u2010mapping mouse model (Rosa26\n                      <jats:sup>RFP<\/jats:sup>\n                      \u00d7 Tbx21\n                      <jats:sup>Cre)<\/jats:sup>\n                      to track developmental and functional reprogramming of NK cells in the uterus, decidua, and placenta throughout pregnancy. Analyses included flow cytometry, bulk RNA sequencing of fate\u2010mapped cells, and single\u2010cell transcriptomic profiling of CD45+Lineage\u2010 immune populations at mid and late gestation.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>We found that NK cells with a history of T\u2010bet expression (RFP+) progressively downregulate T\u2010bet in a tissue and gestation\u2010specific manner, particularly within decidual and placental compartments. Despite this loss, RFP+ cells retained core NK cell markers and altered their lineage identity towards ILC2 or ILC3 fate. Bulk transcriptomic analysis revealed that T\u2010bet downregulation is associated with dampened IFN\u2010\u03b3, and cytotoxic pathways and increased expression of tissue\u2010residency associated transcriptional regulators. Single\u2010cell RNAseq revealed a gestational transition in dNK subset composition, with a decline in cytotoxic tissue\u2010resident NK cells and expansion of regulatory and conventional NK subsets by late gestation.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>These findings identify a novel transcriptional program that shapes NK cell plasticity in response to T\u2010bet downregulation across gestation. Rather than undergoing lineage diversion, dNK cells adapt to the decidual environment via transcriptional compensation and subset redistribution during pregnancy. This work sheds light on the temporal coordination of innate immune function relevant to pregnancy success.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/aji.70220","type":"journal-article","created":{"date-parts":[[2026,3,8]],"date-time":"2026-03-08T09:47:17Z","timestamp":1772963237000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["T\u2010bet Fate Mapping Reveals Gestational Stage\u2010Specific Transcriptional Adaptation of Decidual NK Cells"],"prefix":"10.1111","volume":"95","author":[{"ORCID":"https:\/\/orcid.org\/0009-0006-4014-887X","authenticated-orcid":false,"given":"Mona A.","family":"Mohamed","sequence":"first","affiliation":[{"name":"Division of Reproductive Sciences Department of Obstetrics and Gynecology University of Wisconsin\u2010Madison  Madison Wisconsin 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