{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,15]],"date-time":"2026-07-15T16:36:53Z","timestamp":1784133413904,"version":"3.55.0"},"reference-count":68,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2026,2,27]],"date-time":"2026-02-27T00:00:00Z","timestamp":1772150400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"},{"start":{"date-parts":[[2026,2,27]],"date-time":"2026-02-27T00:00:00Z","timestamp":1772150400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100000024","name":"Canadian Institutes of Health Research","doi-asserted-by":"publisher","award":["IBC\u2010154050"],"award-info":[{"award-number":["IBC\u2010154050"]}],"id":[{"id":"10.13039\/501100000024","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["American J Rep Immunol"],"published-print":{"date-parts":[[2026,3]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Problem<\/jats:title>\n                    <jats:p>\n                      Tissue\u2010resident memory (T\n                      <jats:sub>RM<\/jats:sub>\n                      ) cells represent important immune sentinels that mount rapid recall responses to pathogens and cancers. However, there are limited data in humans on genital tract T\n                      <jats:sub>RM<\/jats:sub>\n                      collected by clinically feasible sampling methods, limiting a full understanding of their role in immunity and clinical disease.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Method of Study<\/jats:title>\n                    <jats:p>We used flow cytometry and single cell RNA sequencing (scRNAseq) to characterize T cells isolated from ectocervical biopsies and endocervical cytobrushes collected from women attending a colposcopy clinic in Winnipeg, Canada.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      The ectocervix generally contained a higher frequency and abundance of immune cells and T\u2010cells compared to the endocervix. CD4+ and CD8+ T\n                      <jats:sub>RM<\/jats:sub>\n                      were more approximately 5\u2010times more frequent and abundant in the ecto\u2010 compared to endocervix, even after accounting for higher T\u2010cell recovery from the ectocervix. Phenotypically, CD4+ T\n                      <jats:sub>RM<\/jats:sub>\n                      showed higher Th17\u2010 and comparable regulatory\u2010associated marker expression compared to non\u2010T\n                      <jats:sub>RM<\/jats:sub>\n                      in both the ecto\u2010 and endocervix. Cervical dysplasia and ectropion were both associated with several immune cell differences in the ecto\u2010 and endocervix including lower CD4+ T\n                      <jats:sub>RM.<\/jats:sub>\n                      Single\u2010cell RNAseq analyses confirmed broad CD69 and core T\n                      <jats:sub>RM<\/jats:sub>\n                      \u2010related gene expression and captured several heterogeneous CD4+ and CD8+ T\n                      <jats:sub>RM<\/jats:sub>\n                      subsets with diverse gene expression and pathways associated with host immunity, homeostasis, and nonimmune cell interactions.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>\n                      Our data suggest that T\n                      <jats:sub>RM<\/jats:sub>\n                      are more abundant in ecto\u2010 versus endocervical samples, which may reflect differences in commonly used sampling methods. Location and heterogeneous expression profiles underscore the need to better understand their role in microbial interactions, inflammation, and genital infection susceptibility in women.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/aji.70222","type":"journal-article","created":{"date-parts":[[2026,3,8]],"date-time":"2026-03-08T21:26:37Z","timestamp":1773005197000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Enumeration, Phenotyping, and Clinical Associations of Tissue\u2010Resident T Cells in the Ecto\u2010 and Endocervix of Women Attending a Colposcopy Clinic"],"prefix":"10.1111","volume":"95","author":[{"given":"Aloysious","family":"Ssemaganda","sequence":"first","affiliation":[{"name":"Department of Medical Microbiology and Infectious Diseases University of Manitoba  Winnipeg Manitoba 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