{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,17]],"date-time":"2026-05-17T14:07:01Z","timestamp":1779026821841,"version":"3.51.4"},"reference-count":80,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2026,4,24]],"date-time":"2026-04-24T00:00:00Z","timestamp":1776988800000},"content-version":"vor","delay-in-days":23,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,4,1]],"date-time":"2026-04-01T00:00:00Z","timestamp":1775001600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["American J Rep Immunol"],"published-print":{"date-parts":[[2026,4]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>Preeclampsia (PE) is a major pregnancy complication that poses risks to both the mother and the fetus. Oxidative stress (OS) plays a crucial role in its pathogenesis. This study aims to explore the diagnostic value of oxidative stress\u2010related genes for PE.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>We integrated single\u2010cell and batch transcriptome data from the GEO database. Through differential expression analysis, WGCNA, and three machine learning algorithms (LASSO, SVM\u2010RFE, and random forest), we screened key genes, constructed a diagnostic model, and analyzed its correlation with immune cells. We used bioinformatics tools to predict regulatory networks. The diagnostic value of key genes was verified through qRT\u2010PCR experiments.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>Our study identified three hub genes\u2014SPP1, CTSC, and SLC9A9\u2014which were predominantly highly expressed in macrophages. The Hub gene and diagnostic model showed preliminary discriminatory ability in distinguishing PE samples from control samples in multiple datasets. Immune infiltration analysis demonstrated a strong positive correlation between macrophages and the three hub genes. Furthermore, cell adhesion emerged as a key signaling pathway in PE progression. FOXC1 was a common transcriptional regulator of all three hub genes, while FOXL1, NFKB1, and POU2F2 co\u2010regulate SLC9A9 and SPP1. qRT\u2010PCR validation confirmed the downregulation of these hub genes in PE.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>This study successfully identified SPP1, CTSC, and SLC9A9 as candidate biomarkers for PE, which may lay the foundation for the development of new monitoring indicators and optimization of targeted therapy strategies.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/aji.70241","type":"journal-article","created":{"date-parts":[[2026,4,24]],"date-time":"2026-04-24T17:40:40Z","timestamp":1777052440000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Identification and Validation of Oxidative Stress\u2010Related Candidate Biomarkers for Preeclampsia via WGCNA and Single\u2010Cell Transcriptomics"],"prefix":"10.1111","volume":"95","author":[{"given":"Wenhua","family":"Ye","sequence":"first","affiliation":[{"name":"Jinan University  Guangzhou Guangdong China"},{"name":"Department of Intensive Care Unit Foshan Women and Children's Hospital  Foshan Guangdong China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0000-6868-2886","authenticated-orcid":false,"given":"Haiyan","family":"Yin","sequence":"additional","affiliation":[{"name":"Department of Intensive Care Unit The First Affiliated Hospital of Jinan University  Guangzhou Guangdong China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2026,4,24]]},"reference":[{"issue":"1","key":"e_1_2_11_2_1","first-page":"e1","article-title":"ACOG Practice Bulletin No 202: Gestational Hypertension and Preeclampsia","volume":"133","author":"Espinoza J. 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