{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,25]],"date-time":"2026-08-25T13:22:02Z","timestamp":1787664122757,"version":"build-2736575974"},"reference-count":46,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2026,7,29]],"date-time":"2026-07-29T00:00:00Z","timestamp":1785283200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,7,29]],"date-time":"2026-07-29T00:00:00Z","timestamp":1785283200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["American J Rep Immunol"],"published-print":{"date-parts":[[2026,8]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Objective<\/jats:title>\n                    <jats:p>Recurrent miscarriage (RM) is a complex pregnancy\u2010related disorder closely associated with impaired extravillous trophoblast (EVT). Antiphospholipid antibody syndrome (APS) is an autoimmune condition that contributes to adverse pregnancy outcomes, including RM. However, the mechanism by which APS induces RM remains largely unclear. Migrasomes, a newly identified type of extracellular vesicle generated during cell migration, may regulate cellular functions through the transfer of microRNAs (miRNAs). Increasing evidence has suggested that migrasomes play important roles in the female reproductive system, particularly in pregnancy\u2010related disorders such as RM. This study aimed to explore the potential role and underlying mechanism of EVT\u2010derived migrasomes in the pathogenesis of RM associated with APS.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>First, EVT\u2010derived migrasomes (EVTDMs) were isolated from an in vitro RM\/APS model established in HTR\u20108\/SVneo cells. Differentially expressed miRNAs in EVTDMs were identified by small RNA sequencing, followed by Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analyses. The predicted target gene of hsa\u2010miR\u2010206 was further validated using a dual\u2010luciferase reporter assay.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>The results showed that migrasome markers were readily detected in EVTDMs derived from HTR\u20108\/SVneo cells. An in vitro RM\/APS model was established by co\u2010incubation with anti\u2010\u03b22\u2010glycoprotein I antibody (anti\u2010\u03b22\u2010GPI Ab). Migrasome\u2010like vesicles were identified by confocal microscopy and transmission electron microscopy. MiRNA profiling revealed that hsa\u2010miR\u2010206 was significantly upregulated in EVTDMs from the RM\/APS group. Based on bioinformatic prediction and dual\u2010luciferase reporter assays, IKBKB was identified as a direct binding target of hsa\u2010miR\u2010206.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>In conclusion, HTR\u20108\/SVneo cells were capable of generating migrasome\u2010like vesicles, and anti\u2010\u03b22\u2010GPI Ab treatment altered the miRNA cargo of EVTDMs. Among the differentially expressed miRNAs, hsa\u2010miR\u2010206 was markedly upregulated and was validated to bind directly to the 3\u2032UTR of IKBKB, suggesting a potential role in posttranscriptional regulation. However, the downstream functional consequences of this interaction remain to be further elucidated.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/aji.70272","type":"journal-article","created":{"date-parts":[[2026,7,29]],"date-time":"2026-07-29T16:20:26Z","timestamp":1785342026000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["EVT\u2010Derived Migrasomes Provide Mechanistic Insights Into Antiphospholipid Syndrome\u2010Associated Recurrent Miscarriage"],"prefix":"10.1111","volume":"96","author":[{"ORCID":"https:\/\/orcid.org\/0009-0009-9913-4220","authenticated-orcid":false,"given":"Di","family":"Wang","sequence":"first","affiliation":[{"name":"Center for Human Reproduction and Genetics The Affiliated Suzhou Hospital of Nanjing Medical University, Suzhou Municipal Hospital, Gusu School, Nanjing Medical University Suzhou China"},{"name":"Department of Obstetrics and Gynecology Shandong Provincial Hospital Affiliated to Shandong First Medical University  Jinan Shandong P.R. China"},{"name":"Department of Obstetrics and Gynecology Shandong Provincial Hospital, Shandong University Jinan Shandong P.R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yu","family":"Xie","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology Shandong Provincial Hospital, Shandong University Jinan Shandong P.R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hongyuan","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology Shandong Provincial Hospital Affiliated to Shandong First Medical University  Jinan Shandong P.R. China"},{"name":"Department of Obstetrics and Gynecology Shandong Provincial Hospital, Shandong University Jinan Shandong P.R. 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China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0964-5695","authenticated-orcid":false,"given":"Yilei","family":"Li","sequence":"additional","affiliation":[{"name":"The First School of Clinical Medicine Shandong University of Traditional Chinese Medicine Jinan Shandong P.R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yan","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology Shandong Provincial Hospital Affiliated to Shandong First Medical University  Jinan Shandong P.R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xietong","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology Shandong Provincial Hospital Affiliated to Shandong First Medical University  Jinan Shandong P.R. 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