{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,26]],"date-time":"2026-07-26T17:00:17Z","timestamp":1785085217061,"version":"3.55.0"},"reference-count":48,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2026,7,3]],"date-time":"2026-07-03T00:00:00Z","timestamp":1783036800000},"content-version":"vor","delay-in-days":2,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,7,1]],"date-time":"2026-07-01T00:00:00Z","timestamp":1782864000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["American J Rep Immunol"],"published-print":{"date-parts":[[2026,7]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Problem<\/jats:title>\n                    <jats:p>Preeclampsia (PE) is a serious human pregnancy complication and remains the most complex hypertensive disorder due to its multifactorial nature and multisystemic effects. Despite the incomplete understanding of the PE pathophysiology, it is well\u2010accepted that placental changes in PE are associated with oxidative stress, increasing the excessive production of cytokines and other inflammatory mediators. Thus, agents such as silibinin, a non\u2010toxic natural polyphenolic flavonoid with antioxidant, anti\u2010inflammatory, and hepatoprotective properties could modulate this profile for better disease resolution. This study aimed to investigate the effect of silibinin treatment on N\u03c9\u2010nitro\u2010L\u2010arginine methyl ester (L\u2010NAME)\u2010induced PE in rats, exploring its potential as a preventive anti\u2010inflammatory agent.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods of Study<\/jats:title>\n                    <jats:p>Pregnant Wistar rats were treated or not during gestation (days 10\u201319) with L\u2010NAME (70\u201380\u00a0mg\/kg\/day) in drinking water and with silibinin (100\u00a0mg\/kg\/day, orally) starting on days 0, 7 or 14 of pregnancy. Systolic blood pressure was recorded on gestation days 0 and 20. The rats were euthanized on day 20. Then, we evaluated proteinuria, maternal weight gain, and litter weight. Tumor necrosis factor\u2010 alpha (TNF\u2010\u03b1), interleukin\u20101 (IL\u20101\u03b2), IL\u20106, IL\u201010, interferon\u2010gamma (IFN\u2010\u03b3), heat shock protein 70 (Hsp70) and NF\u2010\u03baB activity were determined in liver and placenta homogenates.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>Our findings indicated that silibinin treatment decreased the production of the inflammatory cytokines TNF\u2010\u03b1, IL\u20101\u03b2, IFN\u2010\u03b3, and reduced Hsp70 levels in the placenta and liver homogenate. NF\u2010\u03baB activation was also decreased in these organs of silibinin\u2010treated groups, especially in the LN+SB0 and LN+SB7 groups.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>This study introduces a novel use of silibinin as a preventive agent in a PE\u2010like model that reproduces essential features of human PE, providing physiological relevance to the findings. Overall, our data emphasize the translational potential of silibinin for PE prevention, showing promising effects on key inflammatory and stress\u2010related pathways.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/aji.70275","type":"journal-article","created":{"date-parts":[[2026,7,3]],"date-time":"2026-07-03T11:26:39Z","timestamp":1783077999000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Silibinin Modulates NF\u2010\u03baB Activation and Heat Shock Protein in Preeclampsia\u2010Like Rat Model"],"prefix":"10.1111","volume":"96","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-8010-4496","authenticated-orcid":false,"given":"C. F.","family":"Bannwart\u2010Castro","sequence":"first","affiliation":[{"name":"General and Applied Biology Post Graduate Program Institute of Biosciences Sao Paulo State University\u2010UNESP  Botucatu Sao Paulo Brazil"},{"name":"Experimental Research Unity \u2010 UNIPEX Botucatu Medical School Sao Paulo State University\u2010UNESP Botucatu Sao Paulo Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M.","family":"Romao\u2010Veiga","sequence":"additional","affiliation":[{"name":"Department of Gynecology and Obstetrics Botucatu Medical School Sao Paulo State University\u2010UNESP  Botucatu Sao Paulo Brazil"},{"name":"Department of Genetics Microbiology and Immunology Institute of Biosciences Sao Paulo State University\u2010UNESP Botucatu Sao Paulo Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"V. R.","family":"Ribeiro\u2010Vasques","sequence":"additional","affiliation":[{"name":"Department of Gynecology and Obstetrics Botucatu Medical School Sao Paulo State University\u2010UNESP  Botucatu Sao Paulo Brazil"},{"name":"Department of Genetics Microbiology and Immunology Institute of Biosciences Sao Paulo State University\u2010UNESP Botucatu Sao Paulo Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M. T. S.","family":"Peracoli","sequence":"additional","affiliation":[{"name":"Department of Gynecology and Obstetrics Botucatu Medical School Sao Paulo State University\u2010UNESP  Botucatu Sao Paulo Brazil"},{"name":"Department of Genetics Microbiology and Immunology Institute of Biosciences Sao Paulo State University\u2010UNESP Botucatu Sao Paulo Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J. 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