{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,26]],"date-time":"2026-07-26T17:00:18Z","timestamp":1785085218597,"version":"3.55.0"},"reference-count":59,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2026,7,1]],"date-time":"2026-07-01T00:00:00Z","timestamp":1782864000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,7,1]],"date-time":"2026-07-01T00:00:00Z","timestamp":1782864000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["American J Rep Immunol"],"published-print":{"date-parts":[[2026,7]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Problem<\/jats:title>\n                    <jats:p>Dysfunctional autophagy in ovarian granulosa cells (GCs) represents a key pathological feature of insulin resistance (IR) in polycystic ovary syndrome (PCOS), though regulatory roles of autophagy\u2010associated lncRNAs remain poorly characterized.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Method of Study<\/jats:title>\n                    <jats:p>Comparing GCs from IR\u2010PCOS patients, non\u2010IR PCOS patients, and non\u2010PCOS controls revealed significantly elevated LUCAT1 expression in IR\u2010PCOS. Functional validation via LUCAT1 knockdown in primary IR\u2010PCOS GCs assessed cell viability, apoptosis, autophagy markers, and insulin sensitivity. Mechanistic studies employed Ago2\u2010RIP, dual\u2010luciferase reporter assays, and rescue experiments with miR\u201019a\u20103p mimic or HMGB1 overexpression.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>LUCAT1 knockdown promoted cell viability, reduced cell apoptosis, suppressed autophagy (decreased LC3B puncta, reduced LC3II\/LC3I ratio, elevated p62), enhanced insulin sensitivity (upregulated IRS1, promoted GLUT4 membrane translocation, increased glucose uptake), and restored the secretion of steroid hormones (estradiol and progesterone) in GCs. Mechanistically, LUCAT1 functioned as a molecular sponge for miR\u201019a\u20103p, thereby increasing HMGB1 expression. MiR\u201019a\u20103p mimic replicated LUCAT1 knockdown effects, while HMGB1 overexpression abolished these phenotypes.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>Altogether, LUCAT1 knockdown reduces autophagy, apoptosis, dysfunction, and IR of GCs from PCOS\u2010IR patients through modulating the miR\u201019a\u20103p\/HMGB1 axis.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Trial Registration<\/jats:title>\n                    <jats:p>This study is a retrospective observational clinical study. No prospective study\u2010designed interventions, additional clinical treatments, or randomized controlled trials were performed in this work. In accordance with the recommendations of the International Committee of Medical Journal Editors (ICMJE), formal clinical trial registration is not mandatory for retrospective observational studies without trial intervention. Thus, no trial registration number is available for this study.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/aji.70278","type":"journal-article","created":{"date-parts":[[2026,7,1]],"date-time":"2026-07-01T14:38:36Z","timestamp":1782916716000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Targeting Long Noncoding RNA LUCAT1 Alleviates Insulin Resistance of Ovarian Granulosa Cells in Polycystic Ovary Syndrome by Blocking HMGB1\u2010Mediated Autophagy"],"prefix":"10.1111","volume":"96","author":[{"given":"Jing","family":"Pan","sequence":"first","affiliation":[{"name":"Gynecology Department the Second Affiliated Hospital of Xinjiang Medical University  Urumqi China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Riyangul.","family":"kurban","sequence":"additional","affiliation":[{"name":"Gynecology Department the Second Affiliated Hospital of Xinjiang Medical University  Urumqi China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0006-6010-0697","authenticated-orcid":false,"given":"Xiaoli","family":"Bo","sequence":"additional","affiliation":[{"name":"Gynecology Department the Second Affiliated Hospital of Xinjiang Medical University  Urumqi China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,7,1]]},"reference":[{"key":"e_1_2_11_2_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms23084110"},{"key":"e_1_2_11_3_1","doi-asserted-by":"publisher","DOI":"10.1093\/humupd\/dmy007"},{"key":"e_1_2_11_4_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12958-021-00873-3"},{"key":"e_1_2_11_5_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms23084334"},{"key":"e_1_2_11_6_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpendo.00034.2021"},{"key":"e_1_2_11_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.repbio.2021.100594"},{"key":"e_1_2_11_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biopha.2021.112288"},{"key":"e_1_2_11_9_1","doi-asserted-by":"publisher","DOI":"10.1042\/CS20220408"},{"key":"e_1_2_11_10_1","doi-asserted-by":"publisher","DOI":"10.1080\/15548627.2021.1938914"},{"key":"e_1_2_11_11_1","doi-asserted-by":"publisher","DOI":"10.18632\/oncotarget.9590"},{"key":"e_1_2_11_12_1","doi-asserted-by":"publisher","DOI":"10.2174\/1573399812666160217122530"},{"key":"e_1_2_11_13_1","doi-asserted-by":"publisher","DOI":"10.3389\/fendo.2022.1091147"},{"key":"e_1_2_11_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.freeradbiomed.2022.05.010"},{"key":"e_1_2_11_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jep.2021.113821"},{"key":"e_1_2_11_16_1","doi-asserted-by":"publisher","DOI":"10.1530\/REP-20-0643"},{"key":"e_1_2_11_17_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpendo.00006.2020"},{"key":"e_1_2_11_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biopha.2020.111158"},{"issue":"4","key":"e_1_2_11_19_1","first-page":"967","article-title":"Long Non\u2011Coding RNA LUCAT1 Contributes to Cisplatin Resistance by Regulating the miR\u2011514a\u20113p\/ULK1 Axis in Human Non\u2011Small Cell Lung Cancer","volume":"57","author":"Shen Q.","year":"2020","journal-title":"International Journal of Oncology"},{"key":"e_1_2_11_20_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12967-024-05966-2"},{"key":"e_1_2_11_21_1","doi-asserted-by":"publisher","DOI":"10.1002\/cbin.11270"},{"key":"e_1_2_11_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrc.2018.07.165"},{"key":"e_1_2_11_23_1","doi-asserted-by":"publisher","DOI":"10.1155\/2020\/3801748"},{"key":"e_1_2_11_24_1","doi-asserted-by":"publisher","DOI":"10.1111\/febs.16678"},{"key":"e_1_2_11_25_1","doi-asserted-by":"publisher","DOI":"10.1007\/s40618-023-02119-y"},{"key":"e_1_2_11_26_1","doi-asserted-by":"publisher","DOI":"10.1530\/REP-22-0449"},{"key":"e_1_2_11_27_1","doi-asserted-by":"publisher","DOI":"10.1530\/VB-19-0013"},{"key":"e_1_2_11_28_1","doi-asserted-by":"publisher","DOI":"10.1177\/1933719114549852"},{"key":"e_1_2_11_29_1","doi-asserted-by":"publisher","DOI":"10.1093\/humrep\/deh098"},{"key":"e_1_2_11_30_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkz757"},{"key":"e_1_2_11_31_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13059-019-1629-z"},{"key":"e_1_2_11_32_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mce.2019.110697"},{"key":"e_1_2_11_33_1","doi-asserted-by":"publisher","DOI":"10.3389\/fcell.2021.779718"},{"key":"e_1_2_11_34_1","doi-asserted-by":"publisher","DOI":"10.1038\/srep35438"},{"key":"e_1_2_11_35_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11010-017-3235-2"},{"issue":"2","key":"e_1_2_11_36_1","article-title":"Esculin Ameliorates Obesity\u2010Induced Insulin Resistance by Improving Adipose Tissue Remodeling and Activating the IRS1\/PI3K\/AKT\/GLUT4 Pathway","volume":"319","author":"Yang Y. 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