{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,16]],"date-time":"2026-09-16T05:06:53Z","timestamp":1789535213921,"version":"build-2803163510"},"reference-count":74,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2026,8,7]],"date-time":"2026-08-07T00:00:00Z","timestamp":1786060800000},"content-version":"vor","delay-in-days":6,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"},{"start":{"date-parts":[[2026,8,1]],"date-time":"2026-08-01T00:00:00Z","timestamp":1785542400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100000038","name":"Natural Sciences and Engineering Research Council of Canada","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100000038","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["American J Rep Immunol"],"published-print":{"date-parts":[[2026,8]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Problem<\/jats:title>\n                    <jats:p>Maternal obesity and viral infection induce placental inflammation, but how their co\u2010exposure influence fetoplacental development remains unclear. We hypothesised that maternal high fat (HF) diet and viral infection would independently induce placental inflammation and lipid peroxidation, reduce antioxidant defence, and cellular turnover. Further, HF diet would compromise placental capacity to adapt to infection.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Method of Study<\/jats:title>\n                    <jats:p>\n                      Female C57BL\/6J mice were fed a control (CON) or 62% HF diet six weeks before and throughout pregnancy and injected with poly(I:C) (viral mimic) or vehicle (VEH) 24 h before sacrifice at gestational days (GD) 12.5, 15.5, and 18.5 (\n                      <jats:italic>n<\/jats:italic>\n                      = 5\u20138\/group\/GD). Placental inflammasome (NLRP3), oxidative stress (4\u2010HNE), antioxidant defence (GPx\u20104), and cellular proliferation\u2010to\u2010death ratio (Ki\u201067, Caspase\u20103) were assessed by immunohistochemistry, and mRNA expression of\n                      <jats:italic>Tlr3<\/jats:italic>\n                      ,\n                      <jats:italic>Irf3<\/jats:italic>\n                      ,\n                      <jats:italic>Tlr4<\/jats:italic>\n                      ,\n                      <jats:italic>Tirap<\/jats:italic>\n                      , and\n                      <jats:italic>Il\u20101\u03b2<\/jats:italic>\n                      were measured by qPCR. Data were analysed by linear mixed models (\n                      <jats:italic>p<\/jats:italic>\n                      \u2264 0.05).\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      At GD12.5, infection was associated with increased\n                      <jats:italic>Tlr3<\/jats:italic>\n                      mRNA and immunoreactive (ir)\u20104\u2010HNE, and reduced ir\u2010GPx\u20104 expression in the placental labyrinth zone (LZ). By GD15.5, HF diet was associated with increased ir\u2010NLRP3 in both LZ and junctional zones (JZ). Exposure to infection alone and co\u2010exposure to HF diet and infection further increased LZ ir\u2010NLRP3. At GD18.5, HF diet was associated with increased\n                      <jats:italic>Tirap<\/jats:italic>\n                      and\n                      <jats:italic>Il\u20101\u03b2<\/jats:italic>\n                      mRNA expression, ir\u20104\u2010HNE in the JZ and ir\u2010Caspase\u20103 in the LZ.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>Maternal HF diet and infection exert distinct effects on the placenta across gestation, suggesting that maternal overnutrition might reduce the placenta's capacity to handle adverse exposures, which may increase susceptibility to poor fetal outcomes.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/aji.70290","type":"journal-article","created":{"date-parts":[[2026,8,7]],"date-time":"2026-08-07T13:15:40Z","timestamp":1786108540000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Maternal High Fat Diet and Acute Viral Mimic Exposure Impact Placental Inflammation, Lipid Peroxidation and Cellular Proliferation\u2010to\u2010Death Ratio Across Mouse Pregnancy"],"prefix":"10.1111","volume":"96","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-3126-2751","authenticated-orcid":false,"given":"Thaina","family":"Ferraz","sequence":"first","affiliation":[{"name":"Department of Health Sciences Carleton University  Ottawa Ontario Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lucas","family":"Cardoso","sequence":"additional","affiliation":[{"name":"Department of Health Sciences Carleton University  Ottawa Ontario Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sadra","family":"Mohammadkhani","sequence":"additional","affiliation":[{"name":"Department of Health Sciences Carleton University  Ottawa Ontario Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Enrrico","family":"Bloise","sequence":"additional","affiliation":[{"name":"Department of Morphology Institute of Biological Sciences Federal University of Minas Gerais  Belo Horizonte Minas Gerais Brazil"},{"name":"Department of Physiology University of Toronto  Toronto Ontario Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1494-3221","authenticated-orcid":false,"given":"Kristin L.","family":"Connor","sequence":"additional","affiliation":[{"name":"Department of Health Sciences Carleton University  Ottawa Ontario Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,8,7]]},"reference":[{"issue":"219","key":"e_1_2_10_2_1","first-page":"1","article-title":"Prevalence of Obesity Among Adults and Youth: United States, 2011\u20132014","author":"Ogden C. 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