{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,28]],"date-time":"2026-09-28T08:58:46Z","timestamp":1790585926320,"version":"4.1.0"},"reference-count":45,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2026,9,15]],"date-time":"2026-09-15T00:00:00Z","timestamp":1789430400000},"content-version":"vor","delay-in-days":14,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T00:00:00Z","timestamp":1788220800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100003819","name":"Natural Science Foundation of Hubei Province","doi-asserted-by":"publisher","award":["2021CFB526"],"award-info":[{"award-number":["2021CFB526"]}],"id":[{"id":"10.13039\/501100003819","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["American J Rep Immunol"],"published-print":{"date-parts":[[2026,9]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>Preeclampsia (PE) is a pregnancy\u2010specific syndrome, which has been recognized as a leading cause of maternal and neonatal morbidity and mortality worldwide. Calcifediol (25\u2010hydroxy Vitamin D3) was reported to be closely associated with pregnancy outcomes such as preeclampsia, gestational hypertension, and gestational diabetes. Nevertheless, the specific role of calcifediol remains obscure in PE.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>The pregnant rats were treated with L\u2010NAME (50\u00a0mg\/kg\/day) to establish PE models. Histopathological injury of placental tissues was observed by HE staining. Systolic blood pressure, proteinuria, and urine volume were measured to evaluate the symptoms of PE. The neonatal birth rate, pup's body length and weight were determined to assess pregnancy outcomes. The placental cytokines and serum sFlt\u20101\/PIGF ratio were detected by ELISA analysis. The protein levels were measured by Western blotting analysis.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>Calcifediol treatment alleviated the histopathological injury placental tissues of PE rats. In addition, calcifediol improves PE symptoms by attenuating hypertension, proteinuria and urine volume. Besides, calcifediol ameliorated pregnancy outcomes by enhancing live birth rate, neonatal birth length, and neonatal birth weight. Moreover, calcifediol restrained pro\u2010inflammatory cytokines in placental tissues and facilitated serum sFlt\u20101\/PIGF balance in PE rats. Mechanistically, calcifediol is closely associated with the inactivation of JAK\/STAT signaling, as evidenced by the reduction of the phosphorylated JAK1, STAT1, and STAT3 proteins in placental tissues.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>Calcifediol elicits significant therapeutic effects in L\u2010NAME\u2010induced PE rat models by ameliorating PE symptoms and pregnancy outcomes, attenuating inflammatory responses and alleviating histopathological damage.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/aji.70306","type":"journal-article","created":{"date-parts":[[2026,9,15]],"date-time":"2026-09-15T08:53:42Z","timestamp":1789462422000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Calcifediol Suppressed Placental Inflammation and Improved Pregnancy Outcomes by Targeting JAK\/STAT Signaling Pathway in Preeclampsia"],"prefix":"10.1111","volume":"96","author":[{"given":"Mengxue","family":"Wu","sequence":"first","affiliation":[{"name":"Department of Obstetrics and Gynecology, The Central Hospital of Wuhan, Tongji Medical College Huazhong University of Science and Technology  Wuhan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jing","family":"Cai","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology, The Central Hospital of Wuhan, Tongji Medical College Huazhong University of Science and Technology  Wuhan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0007-1980-899X","authenticated-orcid":false,"given":"Jian","family":"Shen","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology, The Central Hospital of Wuhan, Tongji Medical College Huazhong University of Science and Technology  Wuhan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yu","family":"Zhen","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology, The Central Hospital of Wuhan, Tongji Medical College Huazhong University of Science and Technology  Wuhan China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,9,15]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jacc.2020.08.014"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1007\/978\u20103\u2010319\u201044251\u2010810.1007\/5584_2016_82"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ajog.2021.11.1356"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.siny.2020.101123"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1055\/s-0037-1604471"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1161\/CIRCRESAHA.118.313276"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bpobgyn.2024.102480"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1161\/HYPERTENSIONAHA.120.14781"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bone.2013.10.014"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41430\u2010024\u201001520\u2010x"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00198\u2010018\u20104520\u2010y"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.3390\/nu12061617"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.3390\/nu14091820"},{"issue":"1","key":"e_1_2_9_15_1","first-page":"4","article-title":"Effect of Vitamin D Supplementation on Serum 25\u2010Hydroxyvitamin D and Homeostatic Model of Insulin Resistance Levels in Healthy Pregnancy: A Systematic Review and Meta\u2010Analysis","volume":"48","author":"Sharafi S. 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