{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,28]],"date-time":"2026-09-28T08:58:47Z","timestamp":1790585927034,"version":"4.1.0"},"reference-count":28,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2026,8,31]],"date-time":"2026-08-31T00:00:00Z","timestamp":1788134400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,8,31]],"date-time":"2026-08-31T00:00:00Z","timestamp":1788134400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/100000871","name":"Mayo Clinic","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100000871","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["American J Rep Immunol"],"published-print":{"date-parts":[[2026,9]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Problem<\/jats:title>\n                    <jats:p>\n                      Patients with threatened preterm labor (PTL) are treated with antibiotics, corticosteroids, and magnesium sulfate to mitigate infection, promote fetal lung maturation, provide fetal neuroprotection and overall minimize the impact of prematurity on the neonate. However, the effect of these drugs on the placental inflammatory profile is unknown even though these are given systemically and reach the placenta. We evaluated their action in an\n                      <jats:italic>ex vivo<\/jats:italic>\n                      model of placental inflammation.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Method of Study<\/jats:title>\n                    <jats:p>Placental explants from uncomplicated pregnancies were exposed to lipopolysaccharide (LPS) \u00b1 azithromycin, betamethasone, and\/or magnesium sulfate. Production (lysate) and secretion (supernatant) of pro\u2010 and anti\u2010inflammatory cytokines were assessed by ELISA.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>Both pro\u2010 and anti\u2010inflammatory cytokines were increased by LPS versus untreated. Azithromycin, or magnesium sulfate did not alter the inflammatory profile by themselves nor when added at the same time or 24\u00a0h after LPS. Betamethasone decreased both pro\u2010 and anti\u2010inflammatory cytokines only when administered at the same time as LPS, without any effects if delayed.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>\n                      Therapeutic interventions classically used for preterm labor (PTL) do not impact the placental inflammatory profile by themselves or in the context of an\n                      <jats:italic>ex vivo<\/jats:italic>\n                      placental explants model of LPS\u2010induced inflammation, except for betamethasone when administered concurrently with LPS.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/aji.70316","type":"journal-article","created":{"date-parts":[[2026,8,31]],"date-time":"2026-08-31T09:00:37Z","timestamp":1788166837000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Effects of Common Therapies for Threatened Preterm Labor on the Placental Inflammatory Response"],"prefix":"10.1111","volume":"96","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-8171-2223","authenticated-orcid":false,"given":"Jessica","family":"Weng","sequence":"first","affiliation":[{"name":"Mayo Clinic Medical Scientist Training Program, Mayo Clinic Graduate School of Biomedical Sciences Mayo Clinic Alix School of Medicine  Rochester Minnesota USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0006-5239-7056","authenticated-orcid":false,"given":"Francisco","family":"Cortina","sequence":"additional","affiliation":[{"name":"Department of Immunology Mayo Clinic  Rochester Minnesota USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0007-4267-0642","authenticated-orcid":false,"given":"Suyun","family":"Ling","sequence":"additional","affiliation":[{"name":"Department of Immunology Mayo Clinic  Rochester Minnesota USA"},{"name":"Department of OB\u2010GYN Mayo Clinic  Rochester Minnesota USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5164-5816","authenticated-orcid":false,"given":"Sylvie","family":"Girard","sequence":"additional","affiliation":[{"name":"Department of Immunology Mayo Clinic  Rochester Minnesota USA"},{"name":"Department of OB\u2010GYN Mayo Clinic  Rochester Minnesota USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,8,31]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1001\/jamapediatrics.2022.1622"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa1516783"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1002\/14651858.CD004454.pub2"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1155\/2020\/2093530"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12884\u2010024\u201006703\u20109"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.phrs.2012.06.011"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1186\/1465\u20109921\u201010\u2010124"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.1101765"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.3390\/cells12020209"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ajog.2008.11.034"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.pedneo.2013.05.007"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.3390\/cells14130965"},{"key":"e_1_2_9_14_1","doi-asserted-by":"crossref","unstructured":"American College of Obstetricians and Gynecologists. ACOG Practice Bulletin no. 127: Management of Preterm Labor. 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Prevention of Group B Streptococcal Early\u2010Onset Disease in Newborns: ACOG Committee Opinion Number 797. Obstetrics and Gynecology2020;135 no.2:e51\u2013e72 https:\/\/doi.org\/10.1097\/aog.0000000000003668.","DOI":"10.1097\/AOG.0000000000003668"},{"key":"e_1_2_9_28_1","doi-asserted-by":"publisher","DOI":"10.1165\/rcmb.2008\u20100155OC"},{"key":"e_1_2_9_29_1","doi-asserted-by":"publisher","DOI":"10.1002\/prp2.58"}],"container-title":["American Journal of Reproductive Immunology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/aji.70316","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/aji.70316","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/aji.70316","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,9,28]],"date-time":"2026-09-28T08:34:53Z","timestamp":1790584493000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/aji.70316"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,8,31]]},"references-count":28,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2026,9]]}},"alternative-id":["10.1111\/aji.70316"],"URL":"https:\/\/doi.org\/10.1111\/aji.70316","archive":["Portico"],"relation":{},"ISSN":["1046-7408","1600-0897"],"issn-type":[{"value":"1046-7408","type":"print"},{"value":"1600-0897","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,8,31]]},"assertion":[{"value":"2026-01-05","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-08-20","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-08-31","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e70316"}}