{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,14]],"date-time":"2026-10-14T14:32:46Z","timestamp":1791988366389,"version":"4.4.0"},"reference-count":20,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2013,1,15]],"date-time":"2013-01-15T00:00:00Z","timestamp":1358208000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["obgyn.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Aust NZ J Obst Gynaeco"],"published-print":{"date-parts":[[2013,6]]},"abstract":"Aims<\/jats:title>Lower vaginal swabs were collected to compare the intrapartum polymerase chain reaction (PCR<\/jats:styled-content>) test to the standard antepartum culture for detection of group B<\/jats:styled-content> streptococcus (GBS<\/jats:styled-content>) colonisation.<\/jats:p><\/jats:sec>Materials and Methods<\/jats:title>Four hundred and forty\u2010five pregnant women with documented antepartum GBS<\/jats:styled-content> cultures, who did not receive either antepartum or intrapartum antibiotics, were included in this prospective study. At the time of delivery and before the intrapartum antibiotic prophylaxis (IAP<\/jats:styled-content>), double swabs were collected, one of the swab was used in the GBS<\/jats:styled-content> molecular\u2010based (PCR<\/jats:styled-content>) test and the other was processed for GBS<\/jats:styled-content> culture in Ahmadi Hospital Clinical Laboratory. Neonates were examined after delivery to diagnose GBS<\/jats:styled-content> sepsis, and using intrapartum GBS<\/jats:styled-content> culture as gold standard test; the intrapartum PCR<\/jats:styled-content> test was compared to the antepartum culture.<\/jats:p><\/jats:sec>Results<\/jats:title>The sensitivity and specificity of antepartum culture to diagnose GBS<\/jats:styled-content> colonisation were 73 & 95.5%, respectively, compared with 98.3% sensitivity & 99% specificity for intrapartum PCR<\/jats:styled-content> test (P<\/jats:italic>\u00a0>\u00a00.05). Although the intrapartum PCR<\/jats:styled-content> test was more accurate (98.8%) for detection of GBS<\/jats:styled-content> colonisation than antepartum culture (90%), its accuracy was significantly different. The neonatal GBS<\/jats:styled-content> sepsis was significantly related to positive intrapartum cultures, and intrapartum PCR<\/jats:styled-content> tests, but was not related to positive antepartum cultures (the IAP<\/jats:styled-content> to the antepartum GBS<\/jats:styled-content>\u2010positive mothers reduces GBS<\/jats:styled-content> sepsis of their neonates).<\/jats:p><\/jats:sec>Discussion<\/jats:title>The intrapartum PCR<\/jats:styled-content> test is an accurate bedside test and it has the potential to be used as intrapartum screening for GBS<\/jats:styled-content> colonisation allowing appropriate management of mothers and neonates.<\/jats:p><\/jats:sec>","DOI":"10.1111\/ajo.12030","type":"journal-article","created":{"date-parts":[[2013,1,15]],"date-time":"2013-01-15T10:53:35Z","timestamp":1358247215000},"page":"236-242","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":18,"title":["Intrapartum polymerase chain reaction for detection of group B streptococcus colonisation"],"prefix":"10.1111","volume":"53","author":[{"given":"Ibrahim A.","family":"Abdelazim","sequence":"first","affiliation":[{"name":"Department of Obstetrics & Gynaecology Ain Shams University Cairo Egypt"},{"name":"Ahmadi Hospital Kuwait Oil Company (KOC) Ahmadi Kuwait"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2013,1,15]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1080\/00016340801908759"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1001\/jama.299.17.2056"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa020205"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1086\/600303"},{"issue":"10","key":"e_1_2_6_6_1","first-page":"1","article-title":"Prevention of perinatal group B streptococcal disease","volume":"59","author":"Centers for Disease Control and Prevention (CDC)","year":"2010","journal-title":"MMWR"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1097\/AOG.0b013e31817f5cb9"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1469-0691.2010.03378.x"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ajog.2007.06.016"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1055\/s-0030-1254237"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.1097\/AOG.0b013e3181dd916f"},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa0806820"},{"key":"e_1_2_6_13_1","doi-asserted-by":"publisher","DOI":"10.1542\/peds.2005-1114"},{"key":"e_1_2_6_14_1","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.44.3.725-728.2006"},{"key":"e_1_2_6_15_1","doi-asserted-by":"publisher","DOI":"10.1097\/01.AOG.0000228961.42272.31"},{"key":"e_1_2_6_16_1","doi-asserted-by":"publisher","DOI":"10.1099\/jmm.0.46731-0"},{"key":"e_1_2_6_17_1","doi-asserted-by":"publisher","DOI":"10.1097\/AOG.0b013e31817710ee"},{"key":"e_1_2_6_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ajog.2011.06.087"},{"key":"e_1_2_6_19_1","doi-asserted-by":"publisher","DOI":"10.1097\/AOG.0b013e318160ff9d"},{"key":"e_1_2_6_20_1","doi-asserted-by":"publisher","DOI":"10.1097\/00003246-200107001-00035"},{"key":"e_1_2_6_21_1","doi-asserted-by":"publisher","DOI":"10.1097\/AOG.0b013e31824b1461"}],"container-title":["Australian and New Zealand Journal of Obstetrics and Gynaecology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fajo.12030","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/obgyn.onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/ajo.12030","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,11,22]],"date-time":"2023-11-22T12:51:27Z","timestamp":1700657487000},"score":1,"resource":{"primary":{"URL":"https:\/\/obgyn.onlinelibrary.wiley.com\/doi\/10.1111\/ajo.12030"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2013,1,15]]},"references-count":20,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2013,6]]}},"alternative-id":["10.1111\/ajo.12030"],"URL":"https:\/\/doi.org\/10.1111\/ajo.12030","archive":["Portico"],"relation":{},"ISSN":["0004-8666","1479-828X"],"issn-type":[{"value":"0004-8666","type":"print"},{"value":"1479-828X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2013,1,15]]},"assertion":[{"value":"2012-08-04","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2012-11-02","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2013-01-15","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}